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J Agric Food Chem ; 61(26): 6282-7, 2013 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-23734915

RESUMO

The acetyl (AcE), feruloyl (FE), and p-coumaroyl (pCE) ester contents of different cereal and grass polysaccharides were determined by a quantitative ¹H NMR-based method. The repeatability and the robustness of the method were demonstrated by analyzing different plant polysaccharide preparations. Good sensitivity and selectivity for AcE, FE, and pCE were observed. Moreover, an optimized and easy sample preparation allowed for simultaneous quantification of AcE, FE, and pCE. The method is suitable for high-throughput analysis, and it is a good alternative for currently used analytical procedures. A comparison of the method presented to a conventional HPLC-based method showed that the results obtained are in good agreement, whereas the combination of the optimized sample preparation and analysis by the ¹H NMR-based methodology results in significantly reduced analysis time.


Assuntos
Parede Celular/química , Produtos Agrícolas/química , Análise de Alimentos/métodos , Polissacarídeos/análise , Acetilação , Ração Animal/análise , Parede Celular/metabolismo , Produtos Agrícolas/metabolismo , Ésteres , Países Baixos , Fenóis/análise , Fenóis/química , Polissacarídeos/química , Polissacarídeos/metabolismo
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