RESUMO
This phase I study demonstrates the immunostimulating properties of SLO4 administered by the oral route in the healthy volunteer. Responses to the test of DTH (delayed type hypersensitivity) were enhanced by SLO4 and the production of MIF (migration inhibiting factor) involved in this response was stimulated. These properties could be correlated with the previously demonstrated induction of interferon by SLO4.
Assuntos
Adjuvantes Imunológicos/farmacologia , Hipersensibilidade Tardia/imunologia , Fatores Inibidores da Migração de Leucócitos/sangue , Linfocinas/sangue , Adjuvantes Imunológicos/administração & dosagem , Administração Oral , Ensaios Clínicos como Assunto , Relação Dose-Resposta a Droga , Escherichia coli , Humanos , Cinética , Klebsiella pneumoniae , Testes CutâneosRESUMO
A product isolated from Klebsiella pneumoniae and Escherichia coli, coded SLO4, has been shown to be effective in endogenous interferon induction in vivo in mouse when administered IP or IV, and in vitro with human leukocyte cultures. In these two systems induced interferon was defined. The inducer has not yet been characterized but seems not to belong to any components known to be interferon inducers such as viral particles, nucleic acids or endotoxins. An analytical study will be carried out to specify the constitution of this interferon inducer.
Assuntos
Escherichia coli/imunologia , Indutores de Interferon/isolamento & purificação , Klebsiella pneumoniae/imunologia , Animais , Feminino , Humanos , Interferons/imunologia , Leucócitos/imunologia , Masculino , Camundongos , Camundongos EndogâmicosAssuntos
Escherichia coli/fisiologia , Indutores de Interferon , Interferon Tipo I/biossíntese , Interferon gama/biossíntese , Klebsiella pneumoniae/fisiologia , Leucócitos/metabolismo , 2',5'-Oligoadenilato Sintetase/sangue , Administração Oral , Animais , Células Cultivadas , Feminino , Humanos , Cinética , Leucócitos/enzimologia , Leucócitos/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Fatores de TempoRESUMO
Evaluation of the activity of an enzyme activated by interferon (2'-5' oligo-isoadenylate synthetase or 2-5A synthetase) has been used to determine, in man, the interferon inducing capacity of a fraction isolated from Escherichia coli and Klebsiella pneumoniae, SL04, an immunomodulating agent administrated per os. An increase of the activity of this enzyme has been shown in four out of five volunteers following a single oral administration of 100 mg of SL04. This activation demonstrated in humans confirms the pharmacological results of the interferon induction obtained with SL04 in vivo in mice and in vitro in human cell cultures.
