RESUMO
A method for ABO genotyping originally designed by Lee and Chang [3] and further developed by Akane et al. [7] has been even more simplified and improved. We obtained a rapid, robust, sensitive and low cost method for detection of sequence polymorphism of ABO glycosyltransferase gene by changing the MaeII restriction enzyme for its isoschizomer TaiI and by optimization of the condition during digestion and electrophoretic separation.
Assuntos
Sistema ABO de Grupos Sanguíneos/genética , Polimorfismo Genético , Enzimas de Restrição do DNA , Medicina Legal , Genótipo , Glicosiltransferases/genética , Humanos , Reação em Cadeia da Polimerase , Análise de Sequência de DNARESUMO
A population study on 10 short tandem repeat (STR) loci was performed on 202 unrelated Czech Caucasians. DNA, initially frozen, was thawed and placed FTA paper. The DNA was purified in situ. DNA was amplified by PCR using the AmpFlSTR SGM Plus amplification kit.
Assuntos
Impressões Digitais de DNA/instrumentação , Impressões Digitais de DNA/métodos , Frequência do Gene/genética , Repetições Minissatélites/genética , Reação em Cadeia da Polimerase/instrumentação , Reação em Cadeia da Polimerase/métodos , Tchecoslováquia , Análise Discriminante , Homozigoto , Humanos , Técnicas de Amplificação de Ácido Nucleico/instrumentação , Técnicas de Amplificação de Ácido Nucleico/métodos , População Branca/genéticaRESUMO
Virus-like particles (VLPs) were isolated from the yeast Wickerhamia fluorescens strain CCY61-1-1. The VLPs are approximately 42 nm in diameter and contain only one species of dsRNA molecule. The apparent length of the dsRNA determined by native agarose gel electrophoresis was 4.6 kbp. Analysis of protein content of the VLPs showed them to contain one major capsid protein with an apparent molar mass of 74.5 kDa.
Assuntos
Totivirus/isolamento & purificação , Leveduras/virologia , Capsídeo/isolamento & purificação , Eletroforese em Gel de Ágar , Peso Molecular , RNA de Cadeia Dupla/isolamento & purificação , RNA Viral/isolamento & purificação , Totivirus/genéticaRESUMO
There is a tendency toward hypercoagulability in the postoperative period. This is manifested by changes in a number of coagulation parameters, and if not offset by some protective mechanism, thrombosis may occur. This protection appears to be mediated more through the fibrinolytic mechanism than through the action of antithrombin 3 (AT-3) because AT-3 activity diminishes in the early postoperative period. The introduction of variables such as invasion of the vascular system, intraoperative heparin administration, administration of whole blood, and insertion of a Dacron prosthesis does not appreciably affect the response of the coagulation profile to operative stress.
