RESUMO
In this current work, the herbicidal activity of fungal metabolites stacked chitosan nanocomposite against significant aquatic invasive weed Eichhornia crassipes (water hyacinth) was examined. Herbicidal metabolites from the fungal strain Allophoma oligotrophica were extracted and purified under standard condition. Altered ionic gelation technique was received for the amalgamation of chitosan nanocomposite fabricated with herbicidal metabolites. Synthesized nanocomposite incited checked herbicidal impact on the leaflets of water hyacinth. Synthesized nanocomposite induced marked herbicidal effect on the tested leaflets of water hyacinth. Necrotic development on the tested leaflets at earlier incubation period followed by progressive enhancement of necrotic lesion reveals the noteworthy herbicidal activity of the synthesized nanocomposite. Parenchymal, mesenchymal tissue disintegration, reduction of total chlorophyll content, elevated anti oxidative enzymes and changes in qualitative protein profiling of tested leaflets reveals the nanocomposite induced noteworthy morphometric and functional effects. Effect of solvents on the release profile demonstrates that ethyl acetate treatment brought about controlled or sustained release of metabolites. No sign of toxic effect on the zebra fish embryonic developmental stages revealed biocompatibility of the nanocomposite.
