Microplastic in oysters: A review of global trends and comparison to southern Australia.

Microplastics have been documented in a plethora of marine environments and organisms. These small plastics threaten ecosystem health, with implications for seafood species' health. Oysters are an important cultural and economic aquaculture species globally. Due to their filter feeding mechanisms, they can act as an indicator species and proxy for environmental contamination. This makes them an ideal organism for investigating microplastic pollution. Here, we first systematically reviewed the global literature investigating microplastic in oysters. Globally, 94.4% of all oysters had microplastics, with an average of 1.41 ± 0.33 per gram of soft tissue wet weight (gww). The review showed that wild-caught oysters contained more than double the amount of microplastic than aquaculture raised specimens, likely reflecting the clean and productive waters in which oyster aquaculture systems are commonly located. Second, we quantified microplastic presence and polymer type in commercially farmed oysters (Crassostrea gigas and Saccostrea glomerata) across a broad spatial scale, covering eight sites in southern Australia. Microplastics were present in 49.4% of all sampled oysters, with specimens from all locations containing microplastics. On average, whole oysters contained 0.83 ± 0.08 microplastics per individual or 0.09 ± 0.01 microplastics gww. Using Fourier-Transform Infrared Spectroscopy, we identified that 62% of the verified microplastics were vexar plastic netting, a low-density polyethylene commonly used in aquaculture production. Understanding the abundance and source of microplastic in these key seafood species is essential to determine if oysters are vulnerable to these contaminants and pose a risk to the oyster aquaculture industry as an important food resource.

Multibiomarker responses to polycyclic aromatic hydrocarbons and microplastics in thumbprint emperor Lethrinus harak from a South Pacific locally managed marine area.

To determine the baseline threat of microplastics and polycyclic aromatic hydrocarbons (PAHs) in an important seafood fish from Vueti Navakavu locally managed marine area, a multibiomarker risk assessment was conducted on the thumbprint emperor fish Lethrinus harak. Condition factor, a measure of relative general health condition of fish, was significantly lower in samples from the wet season compared to the dry season but no significant differences were observed for hepatosomatic index, a measure of relative stored energy/nutrition, between seasonal groups. PAHs levels of four metabolites in emperor fish from Fiji waters are reported here for the first time; seasonal groups showed no significant differences, but all samples presented levels of biliary PAHs. Each specimen also contained at least one microplastic in its gastrointestinal system; fibres were the predominant form-type and ingestion levels showed that more than 80% of fragment sizes were below 1.0 mm. Biochemical responses were observed for ethoxyresorufin-O-deethylase and glutathione S-transferase biotransformation activity, oxidative stress (glutathione peroxidase and glutathione reductase activity; lipid peroxidation) and genotoxicity (micronuclei assay). Though there were no statistically significant differences found, there were biological significances that were important to note; relatively low levels of pollutant exposure and low levels of biochemical responses showed enzymes response in thumbprint emperor were as expected to their roles in the body. In this multibiomarker approach, the observation of pollutants presence and histopathological injuries are considered biologically relevant from a toxicological perspective and serve as a baseline for future pollution studies in seafood fishes in Fiji, with site differences and the inclusion of fish species comparison. We recommend adopting a suite of biomarkers in future regional biomonitoring studies to develop holistic baseline information for other marine settings in Fiji and other Pacific Island countries.

Knowledge gaps in ecotoxicology studies of marine environments in Pacific Island Countries and Territories - A systematic review.

The Pacific Island Countries and Territories (PICTs) are heavily dependent on the marine resources for food security, employment, government revenue and economic development, hence the concern about the potential exposure of these resources to pollutants. The main goal of this review was to identify ecotoxicology studies published that were done in PICTs. Four major gaps were identified: i) a quantitative gap, with low number of studies published on the PICTs; ii) a geographic gap, where ecotoxicology studies have unevenly covered the different PICTs; iii) a temporal gap, as no biological effect monitoring study has so far been published for the PICTs; and, iv) a pollutants gap, as all of the PICTs studies focused mainly on environmental monitoring studying on average two types of pollutants (heavy metals and pesticides) per PICT only. We suggest, therefore, the potential risk to the marine environment to be estimated by assessing the fate of pollutants via chemical and biological effect monitoring.