RESUMO
Research-based education at the undergraduate level is ideal for fostering the training of future scientists. In an undergraduate Developmental Biology course, this learning strategy requires the availability of model species and enough research reagents, not only for technique training but also for the development of student original projects. This might be challenging in most countries, where resources are limited. Hence, there is a need to develop low-cost solutions for use in the classroom. In this study, we describe the optimization and use of two low-cost protocols in zebrafish embryos for hands-on practical sessions and project-based learning in a Developmental Biology undergraduate course in Ecuador. These protocols were designed for the practical and experimental learning of vertebrate meroblastic cleavage, gastrulation, and neural crest differentiation. The proposed protocols have been previously described in the literature and use silver nitrate and alcian blue, two relatively inexpensive reagents, to label cell membranes and cartilage. The silver nitrate protocol allows the study of cell contact formation during cleavage and the identification of cellular changes during gastrulation, including yolk internalization and epiboly. The alcian blue staining allows the analysis of cranial mesenchymal differentiation into cartilage. These protocols are ideal for practical sessions due to their ease of application, quick results, adaptability to the class schedule, and robustness in the hands of beginning researchers. Finally, these protocols are adaptable for research-based class projects.
Assuntos
Nitrato de Prata , Peixe-Zebra , Humanos , Animais , Equador , Azul Alciano , Biologia do DesenvolvimentoRESUMO
El término "fascitis necrotizante" es ampliamente utilizado para designar en forma genérica las infecciones necrotizantes o gangrenosas, de etiología típicamente poli-bacteriana, es una infección poco común de la partes blandas, usualmente causada por bacterias virulentas que producen toxinas y que se caracterizan por afectar a la fascia superficial, tejido subcutáneo, grasa subcutánea con nervios, arterias y venas y fascia profunda. Se acompaña de dolor local, fiebre y toxicidad sistémica. Se realiza una revisión actualizada del tema y la presentación de un caso diagnosticado en nuestro servicio con una celulitisfacial Odontógena posquirúrgica del 38, que evolucionó hacia una fascitis necrotizante de la porción inferior del cuello y región pectoral.
The term Necrotizing fasciitis is widely utilised to generically design gangrenous or necrotizing infections of a probable multibacterial ethiology. It is an uncommon soft tissue infection caused by violent bacteria which produce toxins and are capable of affecting the superficial fascia, subcutaneous tissue, subcutaneous adipose tissue with nerves, arteries and veins and the profound fascia. It is accompanied by local pain, fever and systemic toxicity. We review the topic and present the case diagnosed in our service as a post surgical Facial Odontogenic Cellulites of the 38 that evolved to a necrotizing fasciitis of the neck and pectoral area.
RESUMO
El término fascitis necrotizante es ampliamente utilizado para designar en forma genérica las infecciones necrotizantes o gangrenosas, de etiología típicamente poli-bacteriana, es una infección poco común de la partes blandas, usualmente causada por bacterias virulentas que producen toxinas y que se caracterizan por afectar a la fascia superficial, tejido subcutáneo, grasa subcutánea con nervios, arterias y venas y fascia profunda. Se acompaña de dolor local, fiebre y toxicidad sistémica. Se realiza una revisión actualizada del tema y la presentación de un caso diagnosticado en nuestro servicio con una celulitisfacial Odontógena posquirúrgica del 38, que evolucionó hacia una fascitis necrotizante de la porción inferior del cuello y región pectoral(AU)
The term Necrotizing fasciitis is widely utilised to generically design gangrenous or necrotizing infections of a probable multibacterial ethiology. It is an uncommon soft tissue infection caused by violent bacteria which produce toxins and are capable of affecting the superficial fascia, subcutaneous tissue, subcutaneous adipose tissue with nerves, arteries and veins and the profound fascia.It is accompanied by local pain, fever and systemic toxicity. We review the topic and present the case diagnosed in our service as a post surgical Facial Odontogenic Cellulites of the 38 that evolved to a necrotizing fasciitis of the neck and pectoral area(AU)
