1.
Anal Biochem
; 100(1): 129-35, 1979 Nov 15.
Artigo
em Inglês
| MEDLINE
| ID: mdl-396814
2.
Nucleic Acids Res
; 5(10): 3549-63, 1978 Oct.
Artigo
em Inglês
| MEDLINE
| ID: mdl-724495
RESUMO
Termination of RNA synthesis with 3'-O-Methylnucleoside 5'-triphosphates have been studied using E. coli RNA polymerase holoenzyme and poly [d(A-T)] as well as unfractionated T7 D delta III DNA as templates. It was shown that the termination can be used for DNA sequencing. A sequence of a part of RNA synthesized from AI promoter of the DNA have been determined. Syntheses of four 3'-O-Methylnucleoside 5'-triphosphates are described.