1.
Bioorg Khim
; 22(12): 894-9, 1996 Dec.
Artigo
em Russo
| MEDLINE
| ID: mdl-9054339
RESUMO
A novel, efficient and simple technique that combines subtractive hybridization with kinetic enrichment is proposed for obtaining enriched cDNA. The method is based on the use of a set of special primers that allow for the selective amplification by PCR only of differentially distributed sequences. Using the proposed technique, cDNA of a new gene XEp-1, specifically expressed in the presumptive epidermis of Xenopus laevis, was cloned, starting with the stage of midgastrula.