[Psoriasis and the problem of gene expression regulation]. / Psoriaz i problema reguliatsii ékspressii genov.

During the epidermis maturation different keratin genes are expressed in strictly definite sequences according to the level of epidermis. In psoriasis this sequence in deranged (permutation) Perhaps this is due to implication of homeobox system similar to what occurs in (Ftz) gene. This problem is reviewed and discussed in this paper. All keratin as well as involukrine genes have been sequenced. Regions of K5 gene have been found which play regulatory role in transcription. It has been achieved using transfection K5 into keratinocytes. Psoriatic injury reversed by yEPP peptide of chick Y. sack cells. It is well established that protooncogenes are involved in transcription regulation. Expression of c-myc is increased in psoriatic plagues whereas that of c-fos and c-jun is decreased. This fact may be regarded as an indirect evidence for abnormal functioning c-myc in regulation. It is not ruled out that the system implicated in shedding in reptiles recapitulate in psoriasis.

[Immunosedimentation analysis of proteins]. / Immunosedimentatsionnyi analiz belkov.

After performing experiments with protein sedimentation the ultracentrifuge test-tube was attached to the inferior opening of the channel in the agar block with a vertical gradient of saccharose concentration. The liquid with protein zones was removed from the test-tube to the channel, forcing a 60% solution of saccharose beneath the lower layers in the test-tube. Horizontal electrophoresis was performed so that the protein zones penetrated the width of the channel walls. A piece of agar gel was cut out from the walls along the whole height of the channel. That piece was placed on the slide and embedded with a mixture of agar and antiserum. Immunoelectrophoresis was made according to Lowrell. The method permits obtaining for the first time continuous sedimentograms during immunodevelopment of the protein zones derived on ultracentrifugation.

[Dynamics of alpha-fetoprotein concentration in mice of different genotypes during the neonatal period]. / Dinamika soderzhaniia alpha-fetoproteina u myshei raznykh genotipov v neonatal'nom periode.

A comparative assay of the alpha-fetoprotein (alpha-FP) level in mice of various genotypes (CBA, C3H, C57BL/Se/Sn, BALB/c, CC57W, AKR and nude--nu/nu) was conducted in the course of 3 weeks of postnatal period. The concentration of alpha-FP reached the following levels: the first day 2(-10)-2(-9); the 5th day 2(-8); the 8th day 2(-7); the 15th day 2(-4); on the 22nd day the level was zero. Nude mice which showed the alpha-FP concentration of 2(-2) on the 15th day were an exception. A conclusion was drawn that the alpha-FP synthesis was based not on the athymia of nude mice per se, but upon other unknown factors.

[The place of beta-fetoproteins among other fetal proteins]. / Mesto beta-fetoproteinov sredi drugikh fetal'nykh belkov

Beta1-Fetoprotein (Fbeta1) and fetal hemoglobin (HbF) were compared with the help of immunodiffusion methods. They were shown to be non-identical although Fbeta1, as well as HbF, is stained by benzidine. Antisera against Fbeta1 do not form the line of precipitation with HbF. Besides, we succeeded to differentiate Fbeta1 from Fbeta2 (they are separated under chromatography on sephadex G-200). Fbeta1 differs from Fbeta2 by benzidine staining: Fbeta1 is benzidine-positive and Fbeta2 is benzidine-negative. The constant parallelism in the appearance of Fbeta2 and a monomeric subunit IgMs of the immunoglobulin molecule IgM. Both the proteins, Fbeta1 and Fbeta2, are present in the serum of a newborn, as well as of a patient with ataxia-teleangiectasia. At the same time erythrocytes of the latter do not contain HbF, as was shown by electrophoresis in polyacrylamide gel.