RESUMO
"Somatic" and "metabolic" antigens prepared from A. farinae and T. casei mites in Auvergne cheese were used for experimental immunisation: the hyper-immune serums obtained permitted 11 to 21 precipitant systems to be distinguished. An enzymatic activity could be identified on certain arcs. Research into antigenic communities showed the existence of 1 to 3 arcs common to the mite antigens, but none with the antigens obtained from the Penicillium cyclopium and Mucor fuscus moulds accompanying the mites. The results obtained thus facilitate an epidemiological investigation among the cheesemakers.
Assuntos
Antígenos/análise , Hipersensibilidade Alimentar/imunologia , Ácaros/imunologia , Queijo , França , Humanos , Doenças Profissionais/imunologiaRESUMO
The soluble somatic antigens of Syngamus trachea were studied by gel electrophoresis and by immunoelectrophoresis, including staining for enzyme activity. Rabbit antiserum prepared by repeated injections of parasite material was used to demonstrate the antigens. Serum obtained after 12-15 weekly injections produced up to 16 precipitation arcs with the S. trachea preparation. Two of the arcs represented reactions against contaminating host material, and enzyme activity was detected in seven arcs. Cross reactions occurred with extracts of Trichinella spiralis (two arcs) and Ascaris lumbricoides (one arc).