RESUMO
This work reports the development and optimization of a flow injection analysis system with fluorescence detection (FIA-FLUO) for gatifloxacin (GFX) determination in organized medium. The analytical system was based on the enhanced fluorescence of gatifloxacin in micellar medium containing sodium dodecyl sulfate (SDS) at pH 6.0. The influence of physical (carrier flow rate, sample volume and volume of reaction coil) and chemical (pH, concentration of buffer and concentration of SDS) parameters that could affect the performance of the FIA system was evaluated in order to reach optimum conditions in terms of sensitivity and analytical throughput. Under optimized conditions, the FIA-FLUO system allowed the injection of 40 samples per hour with a limit of quantification of 72 µg/L and a RSD of 3.5% at 0.20 mg/L. Real samples of commercial pharmaceutical formulations containing GFX were analyzed, and no statistical difference was observed between the results obtained using the developed system and those obtained using the reference method based on high-performance liquid chromatography with UV detection.
Assuntos
Análise de Injeção de Fluxo/instrumentação , Análise de Injeção de Fluxo/métodos , Fluoroquinolonas/análise , Espectrometria de Fluorescência/métodos , Cromatografia Líquida de Alta Pressão/métodos , Desenho de Equipamento , Gatifloxacina , Concentração de Íons de Hidrogênio , Limite de Detecção , Micelas , Soluções Oftálmicas/análise , Sensibilidade e Especificidade , Dodecilsulfato de Sódio , Espectrofotometria UltravioletaRESUMO
This is the first report of a natural infection in the saffron finch Sicalis flaveola (Linnaeus, 1766) captured in Brazil, with the establishment of a new host record for the acuarioid nematode Acuaria mayori Lent, Freitas and Proença, 1945, previously referred in Cyanocorax chrysops (Vieillot, 1818) from Paraguay and Sporophila caerulescens caerulescens (Vieillot, 1823) and C. cyanomelas (Wied, 1821) from Brazil and Myarchus nuttingi (Ridgway, 1883) from Costa Rica.
Assuntos
Canários/parasitologia , Nematoides/isolamento & purificação , Animais , Brasil , Feminino , Masculino , Nematoides/classificaçãoRESUMO
Os autores avaliam a utilizaçäo de um preparado de proteina isolada de soja em 13 crianças portadoras de alergia ao leite de vaca, quanto à aceitaçäo do preparado, regressäo da sintomatologia clínica e desenvolvimento de alergia à soja. A idade média do diagnóstico foi de 10 meses, com início da sintomatologia em média aos 4,6 meses. As manifestaçöes clínicas foram variadas, destacando-se choque anafilático em um caso e síndrome da alergia oral em dois...
