Metal concentrations in the whole blood of farmers in southeast Brazil.

In this study, we measured Ni, Co, Cd, and Pb concentrations in the whole blood of farmers from southeast Brazil to address the factors influencing human exposure in this population. The factors included smoking, alcohol consumption, aging, body composition, gender, and feeding preferences, which were measured through carbon and nitrogen isotopic composition. We also calculated and compared the reference values (RVs) of the measured elements to evaluate ongoing exposure levels. We observed the influence of lifestyle habits on metal exposure; Cd levels were statistically higher in smokers, and alcohol consumption affected only Pb concentrations, with an association also observed with the frequency of alcohol ingestion. The metal levels were positively associated with both isotope values, indicating that feeding may be the dominant source of these elements in this population. We also observed the effect of endogenous sources measured through age, as increased Pb concentrations in both genders and higher Cd levels in older women, which is related to bone, kidney, and liver accumulation. The body mass index was negatively associated with Ni, Co, and Cd in women and positively in men. The negative associations may indicate that body fat may act as a reservoir for metals, reducing their availability in the blood in individuals with higher body mass and possibly influencing the assessment of exposure levels. The evaluated population presented elevated RVs for all elements: Ni 36, Co 3.3, Cd 16, and Pb 149 µg L-1. These values point to higher exposure in this population compared to other studies in Brazil and worldwide. These results emphasize an urgent need for monitoring programs for toxic substances in Brazil and evaluating possible health effects, given the ongoing environmental exposure associated with endogenous exposure and lifestyle habits that promote higher metal levels in this population.

Comparative metal accumulation and toxicogenetic damage induction in three neotropical fish species with distinct foraging habits and feeding preferences.

Environmental pollutants affect fish species differently because their routes of exposure make them more critical to a specific group regarding foraging habits or food preferences. However, the association between local problems and environmental conditions makes a particular species more suitable for use in monitoring programs. Thus, this study compared muscle accumulation of metals (Al, Ba, Cr, Cu, Fe, Mn, Ti and Zn) and toxicogenetic damage to three neotropical species from the Itapemirim River Basin (Brazil) with distinct foraging habits and feeding preferences: Geophagus brasiliensis (benthopelagic - omnivorous); Harttia sp. (benthic - detritivorous); and Leporinus copellandii (pelagic - omnivorous). There was seasonal influence on muscle metal concentrations: Ba and Mn concentrations in G. brasiliensis, Cr in Harttia sp. and Zn, Al and Fe in L. copellandii increased during the rainy season. G. brasiliensis was the most sensitive species, since it had a statistically higher prevalence of erythrocytic nuclear abnormalities, which was influenced by seasonality. This result might be an effect of its benthopelagic habits, which favors contact with a larger group of contaminants due to its interaction with both water column and sediment. The present study showed the differential sensitivity of fish species and that the combination of chemical analysis of pollutants with evaluation of toxicogenetic responses helps to choose the best species for field studies.