Custom Design of a Humidifier Chamber for InMeso Crystallization.

Membrane proteins are indispensable for every living organism, yet their structural organization remains underexplored. Despite the recent advancements in single-particle cryogenic electron microscopy and cryogenic electron tomography, which have significantly increased the structural coverage of membrane proteins across various kingdoms, certain scientific methods, such as time-resolved crystallography, still mostly rely on crystallization techniques, such as lipidic cubic phase (LCP) or in meso crystallization. In this study, we present an open-access blueprint for a humidity control chamber designed for LCP/in meso crystallization experiments using a Gryphon crystallization robot. Using this chamber, we have obtained crystals of a transmembrane aspartate transporter GltTk from Thermococcus kodakarensis in a lipidic environment using in meso crystallization. The data collected from these crystals allowed us to perform an analysis of lipids bound to the transporter. With this publication of our open-access design of a humidity chamber, we aim to improve the accessibility of in meso protein crystallization for the scientific community.

Human scent characterization: A review.

Human scent has long been cited as a probable parameter that can be exploited as a biometric measure. Identifying the scent of individual persons using specially trained canines is a well-known forensic method which is frequently used in criminal investigations. To date there has been limited research on the chemical components present in human scent and their usefulness in distinguishing between people. This review delivers insight into studies which have dealt with human scent in forensics. Sample collection methods, sample preparation, instrumental analysis, compounds identified in human scent and data analysis techniques are discussed. Methods for sample collection and preparation are presented, but to date, there is no available validated method. Instrumental methods are presented and from the overview it is clear that gas chromatography combined with mass spectrometry is the method of choice. New developments such as two-dimensional gas chromatography offer exiting possibilities to collect more information. Given the amount and complexity of data, data processing is used to extract the relevant information to discriminate people. Finally, sensors offer new opportunities for the characterization of human scent.