RESUMO
Although investigation with human embryonic stem cells (HESC) is not decreasing, the derivation of new lines has been diminished. The preeminence of only a few HESC lines in research is accompanied by lack of universal applicability of results as well as by genetic under-representation. We previously reported the derivation of one line with male karyotype from Mexican population. Here, we derived one HESC line (Amicqui-2) with female karyotype from poor-quality embryos. These line comply the pluripotent requirements (normal karyotype, detection of pluripotency-associated markers, mycoplasma test and teratoma formation) and could be a valuable model for studying diseases specific to under-represented population.
Assuntos
Técnicas de Cultura de Células/métodos , Embrião de Mamíferos/citologia , Células-Tronco Embrionárias Humanas/citologia , Animais , Linhagem Celular , Feminino , Humanos , México , CamundongosRESUMO
PURPOSE: This study aimed to evaluate interleukin (IL)-1ß concentrations in maternal serum and in embryo-cultured conditioned media and to correlate these findings with success of implantation. METHODS: A total of 70 infertile women who underwent in vitro fertilization treatment were studied. IL-1ß concentrations were quantified in maternal serum and in embryo culture-conditioned media on days 1 and 3. The findings were compared between those who achieved pregnancy and those who did not. RESULTS: No significant differences were found in IL-1ß serum concentrations between the groups. IL-1ß was not detected in day 1 culture-conditioned medium. On day 3, IL-1ß was quantified in 27 patients, and IL-1ß concentrations were significantly higher in women who achieved pregnancy than in those who did not (p < 0.001). CONCLUSIONS: High IL-1ß concentrations in day 3 culture-conditioned medium in patients who achieve pregnancy after in vitro fertilization treatment indicate a possible role of embryonic IL-1ß in the implantation process.
Assuntos
Meios de Cultivo Condicionados/análise , Implantação do Embrião , Fertilização in vitro , Interleucina-1beta/análise , Adulto , Estudos de Coortes , Técnicas de Cultura Embrionária , Feminino , Humanos , Infertilidade Feminina/sangue , Interleucina-1beta/sangue , Gravidez , Taxa de Gravidez , Estudos ProspectivosRESUMO
Data from the literature suggest that human embryonic stem cell (hESC) lines used in research do not genetically represent all human populations. The derivation of hESC through conventional methods involve the destruction of viable human embryos, as well the use of mouse embryonic fibroblasts as a feeder layer, which has several drawbacks. We obtained the hESC line (Amicqui-1) from poor-quality (PQ) embryos derived and maintained on human amniotic epithelial cells (hAEC). This line displays a battery of markers of pluripotency and we demonstrated the capacity of these cells to produce derivates of the three germ layers.
Assuntos
Âmnio/citologia , Técnicas de Cultura Embrionária/métodos , Células Epiteliais/citologia , Células-Tronco Embrionárias Humanas/citologia , Diferenciação Celular , Células Cultivadas , Embrião de Mamíferos/citologia , Células Epiteliais/metabolismo , Células Alimentadoras/citologia , Células-Tronco Embrionárias Humanas/metabolismo , Humanos , Cariotipagem , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismoRESUMO
Objetivo: Analizar el efecto de la albúmina humana sobre la viabilidad espermática. Material y métodos: Se procesaron por el método de swim-up 15 muestras de semen de sujetos sanos con semen normal (criterios de la OMS) utilizando HTF y HTF con albúmina humana al 10 por ciento, en alícuotas de la misma muestra. Al final de la preparación y a las 24 horas se midieron los parámetros de movilidad espermática utilizando el analizador automático Hamilton-Thorn IVOS 2000. Como medida de viabilidad se utilizó el índice de movilidad. Resultados: La velocidad en línea recta (VSL) disminuyó de 17.14 ñ 5.77 um/seg. (media ñ D.E.) a 12.21 ñ 3.86 um/seg, y 12.14 ñ 3.13 um/seg, en las muestras procesadas con y sin albúmina, respectivamente. A 24 horas de la preparación seminal, el IM disminuyó 11.41 por ciento en las muestras preparadas con albúmina y 21.75 por ciento en las muestras seminales que se prepararon con HTF sin albúmina (z = 1.88, p = 0.05). Los valores de la prueba hiposmótica obtenidos con el medio sin albúmina, fueron de 88.85 por ciento ñ 6.90 por ciento y con albúmina de 91.92 por ciento ñ 4.55 por ciento (p = 0.02). La integridad acrosomal también fue mayor en las muestras que se prepararon con albúmina (86.14 por ciento ñ 3.08 por ciento vs. 81.57 por ciento ñ 2.53 por ciento; p < 0.01). Conclusiones: Estos datos sugieren que la albúmina humana en el medio de preparación seminal, protege funcionalmente a los espermatozoides separados por swim-up y aumenta su sobrevida.
