1.
Biochem Biophys Res Commun
; 224(1): 37-42, 1996 Jul 05.
Artigo
em Inglês
| MEDLINE
| ID: mdl-8694830
RESUMO
Partly purified 3 alpha, 7 alpha, 12 alpha-trihydroxy-5 beta-cholestanoyl-CoA oxidase from rabbit liver peroxisomes was found to convert the 25S- but not the 25R diastereoisomer of 3 alpha, 7 alpha, 12 alpha-trihydroxy-5 beta-cholestan-27-oyl-CoA into (24E)-3 alpha, 7 alpha, 12 alpha-trihydroxy-5 beta-cholest-24-en-27-oic acid. In the presence of a peroxisomal THCA-CoA racemase, however, also the 25R isomer was oxidized. Since the mitochondrial steroid-27-hydroxylase, responsible for formation of THCA, is 25R specific a racemase seems to be obligatory for formation of cholic acid by the normal peroxisomal-dependent pathway.
Assuntos
Acil Coenzima A/metabolismo , Colestanóis/metabolismo , Fígado/enzimologia , Microcorpos/enzimologia , Oxirredutases/metabolismo , Animais , Cromatografia em Gel , Cromatografia Líquida de Alta Pressão , Cinética , Masculino , Mitocôndrias Hepáticas/enzimologia , Coelhos , Estereoisomerismo , Especificidade por Substrato
2.
Arch Dis Child
; 48(11): 850-5, 1973 Nov.
Artigo
em Inglês
| MEDLINE
| ID: mdl-4761053