Sequential pretreatment to recover carbohydrates and phosphorus from Desmodesmus sp. cultivated in municipal wastewater.

This study focused on the simultaneous recovery of carbohydrates (CHO) and phosphorus (P) from Desmodesmus sp. biomass cultivated in municipal wastewater, through a sequential pretreatment. The pretreatment consisted first of ultrasound to trigger cell disruption followed by ozonation to recover CHO and P. For ozone pretreatment, three different parameters were considered: ozone concentration (9, 15, 21, 27, 36, and 45 mg O3/L), contact time (15, 25 and 35 min), and pH (8 and 11). The maximum simultaneous release of 84% of CHO and 58% of P was achieved at the experimental parameters of ozone concentration of 45 mg O3/L, contact time of 35 min, and pH of 11. Also, P was concentrated in solution by 8- to 14-fold with respect to municipal wastewater. The sequential pretreatment was conducted at alkaline pH of 11 and atmospheric conditions, which may considerably reduce energy demand and reagents, in comparison to a traditional hydrolysis pretreatment. The results found suggest that the sequential pretreatment could be feasible on a large scale.

Intensified recovery of lipids, proteins, and carbohydrates from wastewater-grown microalgae Desmodesmus sp. by using ultrasound or ozone.

This study evaluates the effect of ultrasound and ozone pretreatments for the subsequent recovery of Desmodesmus sp. biocomponents-lipids, proteins, and carbohydrates-using a response surface methodology. Both pretreatments impact on the recovered lipids quality, solvent waste production and extraction time is analysed for process intensification purposes. For ultrasound pretreatment, independent parameters were energy applied (50-200 kWh/kg dry biomass), biomass concentration (25-75 g/L), and ultrasonic intensity (0.32 and 0.53 W/mL). While for ozone pretreatment, independent parameters were ozone concentration (3-9 mg O3/L), biomass concentration (25-75 g/L), and contact time (5-15 min). In the case of ultrasound pretreatment, recovery yield reached 97 ±â€¯0.4%, 89 ±â€¯3%, and 73 ±â€¯0.6% for proteins, carbohydrates and lipids respectively. Given process required: energy applied of 50 kWh/kg dry biomass, 75 g/L of biomass concentration, 0.32 W/mL of ultrasonic intensity, and 56 min of time process. Ultrasound caused high cell disruption releasing all proteins, thereby obviating downstream processing for its recovery. Ozone pretreatment recovery yield was 85 ±â€¯2%, 48 ±â€¯1.4%, and 25 ±â€¯1.3%, for carbohydrates, lipids and proteins respectively, under the following conditions: 9 mg O3/L of ozone concentration, 25 g/L of biomass concentration, and 5 min of contact time that depicts an energy consumption of 30.64 kWh/kg dry biomass. It was found that ultrasound and ozone pretreatments intensified the lysis and biocomponents recovery process by reducing solvent consumption by at least 92% and extraction time between 80% and 90% compared with extraction of untreated biomass biocomponents. Both pretreatments improve the composition of the recovered lipids. It was noted that the yield of neutral lipids increased from 28% to 67% for ultrasound pretreatment while for ozone pretreatment from 49% to 63%. The method used for lipid extraction may also have an effect but here it was kept constant.

Microbial fuel cells for inexpensive continuous in-situ monitoring of groundwater quality.

Online monitoring of groundwater quality in shallow wells to detect faecal or organic pollution could dramatically improve understanding of health risks in unplanned peri-urban settlements. Microbial fuel cells (MFC) are devices able to generate electricity from the organic matter content in faecal pollution making them suitable as biosensors. In this work, we evaluate the suitability of four microbial fuel cell systems placed in different regions of a groundwater well for the low-cost monitoring of a faecal pollution event. Concepts created include the use of a sediment/bulk liquid MFC (SED/BL), a sediment/sediment MFC (SED/SED), a bulk liquid/air MFC (BL/Air), and a bulk liquid/bulk liquid MFC (BL/BL). MFC electrodes assembly aimed to use inexpensive, durable, materials, which would produce a signal after a contamination event without external energy or chemical inputs. All MFC configurations were responsive to a contamination event, however SED/SED and BL/Air MFC concepts failed to deliver a reproducible output within the tested period of time. BL/BL MFC and SED/BL MFCs presented an increase in the average current after contamination from -0.75 ± 0.35 µA to -0.66 ± 0.41 µA, and 0.07 ± 0.2 mA to 0.11 ± 0.03 mA, respectively. Currents produced by the SED/BL MFC (SMFC) were considerably higher than for the BL/BL MFCs, making them more responsive, readable and graphically visible. A factorial design of experiments (DOE) was applied to evaluate which environmental and design factors had the greatest effect on current response in a contamination event. Within the ranges of variables tested, salinity, temperature and external resistance, only temperature presented a statistically significant effect (p = 0.045). This showed that the biosensor response would be sensitive to fluctuations in temperature but not to changes in salinity, or external resistances produced from placing electrodes at different distances within a groundwater well.

Factors affecting current production in microbial fuel cells using different industrial wastewaters.

This study evaluated how different types of industrial wastewaters (bakery, brewery, paper and dairy) affect the performance of identical microbial fuel cells (MFCs); and the microbial composition and electrochemistry of MFC anodes. MFCs fed with paper wastewater produced the highest current density (125 ± 2 mA/m(2)) at least five times higher than dairy (25 ± 1 mA/m(2)), brewery and bakery wastewaters (10 ± 1 mA/m(2)). Such high current production was independent of substrate degradability. A comprehensive study was conducted to determine the factor driving current production when using the paper effluent. The microbial composition of anodic biofilms differed according to the type of wastewater used, and only MFC anodes fed with paper wastewater showed redox activity at -134 ± 5 mV vs NHE. Electrochemical analysis of this redox activity indicated that anodic bacteria produced a putative electron shuttling compound that increased the electron transfer rate through diffusion, and as a result the overall MFC performance.