RESUMO
A fractional factorial design approach has been used to enhance secondary metabolite production by two Penicillium strains. The method was initially used to improve the production of bioactive extracts as a whole and subsequently to optimize the production of particular bioactive metabolites. Enhancements of over 500% in secondary metabolite production were observed for both P. oxalicum and P. citrinum. Two new alkaloids, citrinalins A (5) and B (6), were isolated and identified from P. citrinum cultures optimized for production of minor metabolites.
Assuntos
Alcaloides/isolamento & purificação , Antibacterianos/isolamento & purificação , Penicillium/metabolismo , Alcaloides/química , Animais , Antibacterianos/química , Antibacterianos/farmacologia , Candida albicans/efeitos dos fármacos , Caulerpa/microbiologia , Cromatografia Líquida de Alta Pressão , Cristalografia por Raios X , Escherichia coli/efeitos dos fármacos , Feminino , Humanos , Biologia Marinha , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Camundongos , Testes de Sensibilidade Microbiana , Conformação Molecular , Estrutura Molecular , Mycobacterium tuberculosis/efeitos dos fármacos , Ressonância Magnética Nuclear Biomolecular , Penicillium/química , Pseudomonas aeruginosa/efeitos dos fármacos , Projetos de PesquisaRESUMO
Chemical investigation of the crude extract of a marine sponge Dysidea robusta led to the isolation of an inseparable mixture of saturated ceramides. These were identified from spectroscopic data as well as by hydrolysis followed by LC-MS analysis of the sphingosine moieties.
Assuntos
Ceramidas/química , Dysidea/química , Acetilação , Animais , Ceramidas/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Espectrometria de Massas por Ionização por Electrospray , Esfingosina/química , Esfingosina/isolamento & purificaçãoRESUMO
Investigation of the bioactive crude extract from the sponge Plakortis angulospiculatus from Brazil led to the isolation of plakortenone ( 1) as a new polyketide, along with five known polyketides ( 2- 6) previously isolated from other Plakortis sponges. The known polyketides were tested in antileishmanial, antitrypanosomal, antineuroinflammatory, and cytotoxicity assays. The results show that plakortide P ( 3) is a potent antiparasitic compound, against both Leishmania chagasi and Trypanosona cruzi, and exhibited antineuroinflammatory activity. The known polyketides 2- 6 were tested for cytotoxicity against four human cancer cell lines, but displayed only moderate cytotoxic activity.