RESUMO
Anaerobic parasitic and free living protozoa and anaerobic rumen fungi often contain a characteristic organelle, the hydrogenosome. Recently obtained molecular data show that hydrogenosomes in parasitic protozoa probably use a mitochondria-like protein targeting mechanism, whereas for hydrogenosomes in fungi a microbody-like mechanism is inferred. Here we present, to our knowledge, the first sequence data of a hydrogenosomal protein in a free-living anaerobic protozoan. It is shown that ferredoxin of the amoeboflagellate Psalteriomonas lanterna is similar to hydrogenosomal ferredoxin of the parasite Trichomonas vaginalis. We suggest that the two ferredoxins use similar organelle targeting mechanisms.
Assuntos
DNA Complementar/isolamento & purificação , Eucariotos/química , Ferredoxinas/genética , Sequência de Aminoácidos , Anaerobiose , Animais , Sequência de Bases , Clonagem Molecular , DNA Complementar/química , Ferredoxinas/química , Dados de Sequência Molecular , Organelas/químicaRESUMO
A study of patients with mild traumatic brain injuries was performed to determine if cognitive screening in the acute care setting can identify patients who will have cognitive dysfunction following discharge to home. While still hospitalized, 166 patients were assessed with a cognitive screening tool. After discharge, telephone follow-up contacts were made by a nurse to assess for subjective reports of cognitive difficulties. A subsample (N = 39) received additional comprehensive cognitive evaluation after discharge. Statistically significant results were noted between cognitive screen and cognitive evaluation results (chi-square = 24.28, p < .0000) and between cognitive screen results and follow-up findings (chi-square = 6.7, p = .0350). Study results support the use of cognitive screening in the acute care setting to identify patients with mild traumatic brain injury who are likely to experience residual cognitive deficits after discharge so appropriate intervention may be planned.
