RESUMO
A study was carried out on 85 patients with lesions of the cervix uteri: 45 carcinomas and 40 dysplasias. A large battery of cytoenzymatic tests was used to determine their potential value in the diagnosis of carcinomas. An increase of LDH, G6PDH, 6PGDH and NADH2-TR activities and a loss of alpha-GP-ase I activity were demonstrated in malignant cells. It is suggested that the cytoenzymologic techniques may offer help in the distinction between normal, dyskaryotic and malignant cells of vaginal fluid. In individual patients these techniques are useful in understanding the evolution of dysplastic lesions and in the diagnosis of carcinoma. These techniques, however, cannot be used as a general screening test for malignancy.
Assuntos
Colo do Útero/enzimologia , Hidrolases/metabolismo , Oxirredutases/metabolismo , Neoplasias do Colo do Útero/enzimologia , Adulto , Fosfatase Alcalina/metabolismo , Feminino , Glucosefosfato Desidrogenase/metabolismo , Humanos , L-Lactato Desidrogenase/metabolismo , Pessoa de Meia-Idade , NADH Tetrazólio Redutase/metabolismo , Fosfogluconato Desidrogenase/metabolismo , Displasia do Colo do Útero/diagnóstico , Displasia do Colo do Útero/enzimologia , Neoplasias do Colo do Útero/diagnósticoRESUMO
Histochemical investigations carried out on 104 breast tumours (10 benign proliferations and 94 carcinomas) showed that breast carcinomas were characterized by various enzymological equipments in relation to tumour differentiation degree, histogenesis, functional stage, site of tumour cells in the carcinomatous islands or cords and to hormonal factors. Tumour cells -- stroma interrelationships study pointed out that high glycolysis (high LDH, NADPH2-TR, NADH2-TR activities) and an intense LEPA-activity of tumour cells seem to be favourable for proliferation and local tumour cells migration.
