Evaluation of pretreatment methods for enzymatic saccharification of wheat straw for bioethanol production.

Pretreatment is an essential step in the enzymatic hydrolysis of biomass and subsequent production of bioethanol. The current study is focused on two different pretreatment methods of wheat straw using mild temperatures (100°C for 2h and RT for overnight). In one method, native substrate was treated with 1.5% (w/v) NaOH at two different above mentioned conditions followed by acid hydrolysis (0.75% (v/v) sulfuric acid at 100°C for 2h). In another method, the native substrate was initially treated with acid (0.75% (v/v) sulfuric acid at 100°C for 2h) followed by treatment with 1.5% (w/v) NaOH at two different above conditions. After the pretreatments, the residues were treated with Accellerase 1500 (26U/g) and maximum yield of glucose (65.2gL(-1)) were found with 0.75% sulfuric acid (100°C for 2h) followed by alkali (1.5% NaOH at 100°C for 2h). Fermentation of this hydrolyzate using Saccharomyces cerevisiae strain produced 24.4gL(-1) of ethanol with corresponding yield of 0.44g/g.

Fungal isolates from natural pectic substrates for polygalacturonase and multienzyme production.

Pectin rich wastes and waste dump yard soils were screened and eighty pectinolytic fungal isolates were obtained by enrichment culturing and ruthenium red plate assay. Eight isolates with higher zones of pectin hydrolysis were selected and tested for polygalacturonase production. One isolate identified as Aspergillus awamori MTCC 9166 with highest polygalacturonase activity was tested for utilization of raw pectins for enzyme production. Polygalacturonase production was high in raw pectin sources like Orange peel (16.8 U/ml) Jack fruit rind (38 U/ml) Carrot peel (36U/ml) and Beet root peel (24U/ml). Selected Aspergillus awamori MTCC 9166 was found to be having good polygalacturonase, xylanase, cellulase and weak amylase and protease activities. This isolate with multi-enzyme production could have application for enzymes production and degradation of fruit and vegetable waste in the process of urban waste disposal.