Purification of phlorotannins from Macrocystis pyrifera using macroporous resins.

Phlorotannins are secondary metabolites produced by brown seaweed, which are known for their nutraceutical and pharmacological properties. The aim of this work was to determine the type of macroporous resin and the conditions of operation that improve the purification of phlorotannins extracted from brown seaweed, Macrocystis pyrifera. For the purification of phlorotannins, six resins (HP-20, SP-850, XAD-7, XAD-16N, XAD-4 and XAD-2) were assessed. The kinetic adsorption allowed determination of an average adsorption time for the resins of 9h. The highest level of purification of phlorotannins was obtained with XAD-16N, 42%, with an adsorption capacity of 183±18mgPGE/g resin, and a desorption ratio of 38.2±7.7%. According to the adsorption isotherm the best temperature of operation was 25°C, and the model that best described the adsorption properties was the Freundlich model. The purification of phlorotannins might expand their use as a bioactive substance in the food, nutraceutical and pharmaceutical industries.

Characterization of pressurized hot water extracts of grape pomace: chemical and biological antioxidant activity.

Pressurized hot water extracts obtained at different temperatures possess different compositions and antioxidant activities and, consequently, different bioactivities. We characterized two pressurized hot water extracts from grape pomace obtained at 100°C (GPE100) and 200°C (GPE200) in terms of antioxidant activity and composition, as well as protective effect on cell growth and mitochondrial membrane potential (Δψm) in a HL-60 cell culture under oxidative conditions. GPE100 extracts were richer in polyphenols and poorer in Maillard reaction products (MRPs) than were GPE200 extracts. Moreover, hydroxymethylfurfural was detected only in GPE200. Both extracts exhibited similar protective effects on cell growth (comparable to the effect of trolox). In addition, GPE100 strongly decreased the Δψm loss, reaching values even lower than those of the control culture. This protective effect may be related to its high polyphenols content. At the highest concentration assessed, both extracts showed strong cytotoxicity, especially GPE200. This cytotoxicity could be related to their MRPs content.