[Characterization of lymphokine-activated killer cells obtained from the natural killers--T cells of patients with hepatic tumor lesion]. / Kharakteristika limfokinaktivirovannykh killerov, poluchennykh iz natural'nykh killerov, -- T-kletok bol'nykh s opukholevym porazheniem pecheni.

To analyze the functions of lymphokine-activated killer cells (LAKC) and to study their phenotype are of paramount importance in the assessment of their role in antitumor immunity. The natural killers--hepatic T cells--have been shown to be used to prepare LAKS that have a higher cytotoxic activity than LAKS that are generalized from peripheral mononuclear cells.

[Antiviral effect of alpha-interferon and cytokine mRNA level in cell cultures infected with a cytopathogenic variant of the hepatitis C virus]. / Protivovirusnyi éffect alpha-interferona i aktivnost' mRNK tsitokinov v kul'turakh kletok, infitsirovannykh tsitopatogennym variantom virusa gepatita C.

An experimental model of the viral C-hepatitis (VCH) infection was worked out in vitro and it was found suitable to study the influence of interferon (IFN) preparations produced on the infection caused by an HCV cytopathogenic variations, i.e. the SW-13 human adrenocarcinoma cellular culture sensitive to the anti-VCH action of alpha-IFN and the MT-4 human lymphoblastoid cellular culture non-sensitive to the anti-VCH action of alpha-IFN. The above cellular models were employed to study, by using the methods of reverse transcription and polymerize chain reaction (RT-PCR), the influence produced by alpha-IFN on the VCH infectious activity as well as to study the changes in the activity of the below cytokine mRNAs: alpha-IFN, gamma-IFN, IL-1 beta, IL-2, IL-4, IL-6, IL-8, IL-10, IL-18 and TNF-alpha. A double treatment of the SW-13 alpha-IFN cellular cultures 24 and 48 hours after the infection was found to essentially suppress (by 4 Ig) reproduction of the VCH cytopathogenic variant. It was detected that the VCH reproduction is mediated by the regulation of a number of cytokine genes. The study results can be a basis for a more effective use of the alpha-IFN preparations in the therapy of VCH-infections.

[Effect of tetracycline on the hepatitis C virus propagation and levels of cytokine mRNA in infected SW-13 and MT-4 cells]. / Vliianie tetratsiklina na infektsionnye svoistva virusa gepatita C i aktivnost' mRNK tsitokinov v infitsirovannykh kul'turakh kletok SW-13 i MT-4.

An experimental HCV-infection model was used in vitro to study the influence of tetracycline on the reproduction of the HCV-infection accompanied by a simultaneous analysis, by using the methods of reverse transcription and polymerase chain reaction (RT-PCR), of the mRNA activity, i.e. alpha-IFN, gamma-IFN, 1 beta-IFN, IL-2, IL-4, IL-6, IL-6, IL-10, IL-12, IL-18 and TNF-alpha. The study was made with the SW-13 long-term cellular cultures (human adrenocarcinoma cells) and MT-4 cell cultures (human lymphoblastoid cells) infected by HCV. An analysis of the obtained data is indicative, in both examined cellular cultures, of a differently-oriented induction-suppression reaction of cytokine mRNAs. Suppression of the activity of the investigated cytokine mRNAs, except for mRNAs IL-18 and TNF-alpha, was observed in the SW-13 cellular culture; while, as for the MT-4 cellular culture, the activity of all studied cytokine mRNAs was pointed out. The results testify to that tetracycline should be studied more actively in experimental infections caused by HCV in vivo.

[Effect of interferon inductors on infection induced by hepatitis C virus and activity of mRNA cytokines in cell cultures SW-13 and MT-4]. / Vliianie induktorov interferona na infektsiiu, vyzvannuiu virusom gepatita C, i aktivnost' mRNK tsitokinov v kul'turakh kletok SW-13 i MT-4.

An experimental model of hepatitis C virus (HCV) infection in cell culture in vitro was used to study the influence of interferon (IFN) inducers on HCV infection activity. In combination with the RT-PCR method, this model was also used to study the dynamics of cytokine mRNA activity for IFN-alpha, IFN-gamma, IL-I beta, IL-2, IL-4, IL-6, IL-8, BL-10, IL-12, IL-18, and TNF-alpha. The research was carried out using long-term cell cultures SW-13 (human paradrenal adenocarcinoma cells) and MT-4 (human cells of lymphoblastoid origin) inoculated with HCV under conditions of acute infection. The obtained data showed that cell cultures SW-13 and MT-4 were sensitive to replication of HCV (cytopathogenic variant). The addition of IFN inducers Savratz, Kagocel, and Cycloferon to infected cell cultures usually resulted in suppression of HCV reproduction in these cultures. Cycloferon had the greatest antiviral activity (virus titer level decreased by a factor of 2.51 g and 5.51 g TCD50 in cell cultures SW-13 and MT-4, respectively). It was suggested that induction of IFN-gamma, IL-4 and IL-8 plays a certain role in HCV reproduction suppression. The results of this work provide an opportunity for more efficacious use of IFN inducers in therapy of HCV infection.