Synthesis of a Bioactive Composition of Chitosan-Selenium Nanoparticles.

A biologically active composition of chitosan-selenium nanoparticles has been developed. Selenium nanoparticles are characterized by a clear bimodal size distribution: 2-3 and ~37 nm. The main active centers of complexation with nanoparticles are the amino and hydroxyl groups of chitosan. In experiments on culturing fibroblasts of the hTERT BJ-5ta cell line on sample films, high biocompatibility of the composition was shown. It was shown that the composition of chitosan-selenium nanoparticles has a corrective effect on the oxidative processes of the body, reducing the activity of free-radical oxidation in the blood of animals. This opens up prospects for the use of this complex in the composition of antioxidant and adaptogenic drugs.

[Changes in the structure of the plasmalemma of hepatocytes and its specialized portions--intercellular junctions in man in complicated forms of cholelithiasis]. / Izmeneniia struktury plazmalemmy gepatotsitov i ee spetsializirovannykh uchastkov--mezhkletochnykh soedinenii u cheloveka pri oslozhnennykh formakh zhelchnokamennoi bolezni.

As demonstrates the electron microscopic investigation of the human liver at complicated forms of cholelithiasis, there is a stage-by-stage destruction of intercellular junctions and a change in the plasmolemma structure of hepatocytes depending on the degree of the disease. The greatest disorder in the plasmolemma structure is observed at gangrenous calculous cholecystitis, as well as at mechanical jaundice and suppurative cholangitis, dependent on choledocholithiasis. The most sensitive are the gap junctions; desmosomes and intermediate junctions are the second to suffer. The structure of the tight junctions is the last to be disturbed. This results in loss of specificity by different areas of plasmolemma in the hepatocytes, and the hepatocytes themselves loose their polarity. These alterations are evidently irriversible and result in death of the cells.