Implementation of a physician-staffed helicopter: impact on time to primary PCI.

AIMS: This study aimed to compare air (AIR) and ground transport (GRD) of STEMI patients bound for primary percutaneous coronary intervention (pPCI). METHODS AND RESULTS: This was a prospective, controlled, observational study, including patients in whom STEMI was suspected outside a 30-minute driving distance from the PCI centre. AIR patients in a 12-month period (May 1, 2010, to April 30, 2011) were compared with GRD patients in a 16-month period (January 1, 2010, to April 30, 2011). The primary endpoint was time from ECG consistent with STEMI to arrival in the cardiac catheterisation laboratory. We included 450 patients, 114 AIR and 336 GRD patients. The median (5-95% range) transport distance was 97 (62-162) vs. 94 (64-172) kilometres, respectively (p=0.01). Time from ECG to cardiac catheterisation laboratory arrival was significantly lower in the AIR group (median 84 minutes (60-160) vs.104 minutes [63-225], p<0.01). Time from ECG to balloon was 114 (78-221) minutes vs.132 (84-262) (p<0.01), respectively. The 30-day mortality was 2.2% (2/91) for AIR and 6.9% (18/262) for GRD patients (p=0.10). One-year mortality was 6.7%, (6/90) vs. 9.9% (26/262) (p=0.35), respectively. CONCLUSIONS: Air transport seemed superior to ground transportation in reducing time from ECG diagnosis to arrival in the catheterisation laboratory for STEMI patients outside a 30-minute driving distance to the PCI centre.

Isolating genes involved with genotoxic drug response in the nematode Caenorhabditis elegans using genome-wide RNAi screening.

The soil nematode Caenorhabditis elegans has become a popular genetic model organism used to study a broad range of complex biological processes, including development, aging, apoptosis, and DNA damage responses. Many genetic tools and tricks have been developed in C. elegans including knock down of gene expression via RNA interference (RNAi). In C. elegans RNAi can effectively be administrated via feeding the nematodes bacteria expressing double-stranded RNA targeting the gene of interest. Several commercial C. elegans RNAi libraries are available and hence gene inactivation using RNAi can relatively easily be performed in a genome-wide fashion. In this chapter we give a protocol for using genome-wide RNAi screening to identify genes involved with the response to genotoxic stress.