Micronucleus test on gas station attendants

In the present study, the micronucleus test was applied in exfoliated cells of buccal mucosa to assess the mutagenicity risk associated with occupational exposure for gas station attendants. For each individual, 2000 exfoliated buccal cells were analyzed for micronucleus frequency. A highly significant difference was found between exposed and control groups. Likewise, a significant difference was found between these groups regarding the frequency of binucleated and broken egg cells. To determine whether smoking, alcohol habit, age, gender, or working time could exert any additional effect, we determined the frequency of micronuclei and binucleated and broken egg cells amongst exposed and control individuals. The results allowed us to conclude that the individuals studied belong to a risk group and should periodically undergo biological monitoring and appropriate care.

Does the length of the interposed segment in the Henley procedure influence duodenogastric reflux? An experimental study.

This study assesses duodenogastric reflux (DGR) using 99mTechnetium-diisopropyl iminodiacetic acid in unoperated dogs and in dogs 28 days after a Billroth II gastrectomy and 28 days after the Henley procedure. Fourteen mongrel dogs were divided randomly into two groups of seven each, according to the length of the interposed jejunal segment used (Group A 10 cm; Group B, 20 cm). All dogs were subjected to a Billroth II gastrectomy, followed by the Henley procedure 30 days later. In Group A, DGR was significantly lower in the preoperative period (median, 0.0%; range, 0.0-0.02%) than after gastrectomy (median, 0.13%; range, 0.08-0.70%) and the Henley procedure (median, 0.12%; range, 0.06-0.22%), but did not differ significantly after gastrectomy and jejunal interposition. In Group B, DGR was significantly higher after gastrectomy (median, 0.15%; range, 0.10-0.64%) than in the preoperative period (median, 0.00%; range, 0.00-0.09%) or than after the Henley procedure (median, 0.00%; range, 0.00-0.7%). DGR did not differ significantly between the preoperative period and after Henley procedure. After jejunal interposition, DGR was significantly higher in Group A than in Group B. The Henley procedure is effective in preventing DGR when the interposed segment is 20 cm in length.