Assuntos
Tipagem de Bacteriófagos , Escherichia coli/classificação , Adulto , Antígenos de Bactérias/genética , Tipagem de Bacteriófagos/métodos , Criança , DNA Bacteriano/genética , Escherichia coli/genética , Escherichia coli/imunologia , Escherichia coli/isolamento & purificação , Infecções por Escherichia coli/microbiologia , Humanos , Peso Molecular , Antígenos O , Plasmídeos , SorotipagemRESUMO
Antibiotic resistance of enterobacterial strains from population isolated in Krasnodar region is rather often controlled by the "plasmid" genes. The conclusion is based on using the colony hybridization with [32P]-DNA fragments of plasmids, carrying the genetic determinants of antibiotic resistance, as a method for antibiotic resistance, genes screening. Kanamycin resistance in the majority of strains is coded by APH (3') II gene, streptomycin resistance by APH (3") gene, chloramphenicol resistance by CATI, sulphonilamide resistance by DHPS type II gene. Tetracycline resistance of the studied enterobacterial strains is not connected with the widespread genetic determinants of a new class tetracycline resistance.
Assuntos
Resistência Microbiana a Medicamentos/genética , Enterobacteriaceae/genética , Genes Bacterianos , Enterobacteriaceae/efeitos dos fármacos , Escherichia coli/efeitos dos fármacos , Escherichia coli/genética , Plasmídeos , Shigella/efeitos dos fármacos , Shigella/genéticaRESUMO
Physical maps of Enterobacteriaceae nonconjugative plasmids pKMR281 (Sm, Su, molecular weight of 6 kb) and pKMR285 (Sm, Su Tc, molecular weight of 9 kb) were constructed for endonucleases EcoRI, PstI, EcoRV, SmaI, BglII, SalI and PvuII. The genes controlling production of aminoglycoside-3''-phosphotransferase and dihydropteroate synthetase of type II and the genetic tetracycline resistance determinant of class A were localized on the plasmids. Heteroduplex analysis of plasmids pKMR281 and pKMR285 showed that plasmid pKMR281 was completely homologous to plasmid pKMR285. The site of plasmid pKMR285 nonhomology with respect to plasmid pKMR281 corresponded to the area containing the tetracycline resistance determinant. Plasmids pKMR281 and pKMR285 were compatible with the tester plasmids of 25 incompatibility groups of Enterobacteriaceae and probably constitute a new incompatibility group. It was shown that pKMR281 and pKMR285 type plasmids were widely distributed in clinical strains of E. coli and Shigella spp. isolated in the Krasnodar Region.
Assuntos
Enterobacteriaceae/genética , Fatores R , Mapeamento Cromossômico , Conjugação Genética , DNA Bacteriano/genética , Resistência Microbiana a Medicamentos/genética , Escherichia coli/genética , Genes Bacterianos , Peso Molecular , Hibridização de Ácido Nucleico , FenótipoAssuntos
Enterobacteriaceae/genética , Pseudomonas aeruginosa/genética , Estreptomicina/antagonistas & inibidores , Sulfanilamidas/antagonistas & inibidores , Resistência Microbiana a Medicamentos/genética , Enterobacteriaceae/efeitos dos fármacos , Enterobacteriaceae/enzimologia , Genes Bacterianos , Plasmídeos , Pseudomonas aeruginosa/efeitos dos fármacos , Pseudomonas aeruginosa/enzimologiaRESUMO
Nonconjugative R-plasmids pBS76 and pBS94 (Sm Su), pBS95 and pBS96 (Sm Su Ap) isolated from clinical strains of Pseudomonas aeruginosa and plasmids pKMR281-pKMN284 (Sm Su), pKMR285-pKMR286 (Sm Su Tc) isolated from clinical strains of enterobacteria have been studied. Restriction maps of these plasmids are presented in the paper with some of plasmid genes for antibiotic resistance localized on them. The resistance determinants of plasmids pBS95 and pBS96 are shown to be included in transposon Tn3612 analogous to Tn3. Plasmids pBS76, pBS94-96 are of the wide host range and belong to incompatibility group P4 (IncQ). Plasmids pKMR281-pKMR286 are mutually incompatible and share the conspicuous DNA homology. They are inherited only by enterobacteria and are compatible with IncQ plasmids but in contrast to them are mobilized by RP4 plasmid with lower frequency.
