1.
Toxicon
; 24(2): 117-22, 1986.
Artigo
em Inglês
| MEDLINE
| ID: mdl-2871642
RESUMO
Gel chromatography with Dyematrex blue B gel and ion exchange chromatography with a methylcellulose cation exchange column have been used to isolate a toxin present in partially purified saline extracts of the dahlia sea anemone Tealia felina. Toxin activity was assessed by its histaminolytic activity. A ten-fold increase in activity per mg protein was achieved in these two steps. Final purification on a Sephacryl column yielded an apparently pure protein with a molecular weight of 7800. The amino acid composition of this compound was determined.