Performance and parasite control of different genetic groups of lambs finished in irrigated pasture / Desempenho e controle parasitário de diferentes grupos genéticos de cordeiros terminados em pastagem irrigada

The aim of this study was to compare the following four genetic groups of hair sheep: Santa Inês (SI), Morada Nova (MN), Brazilian Somali (BS), and the F1 1/2Dorper x 1/2Morada Nova crossbreed on traits related to growth and parasitic infection. Thirty-three male lambs of the same age and of simple birth, under the same pre-weaning management conditions were used in the experiment. After weaning the animals were housed in a completely randomized design in paddocks made of Panicum maximum cv. Tanzania. Along the course of the research, the performance of the four groups of sheep was observed to be negatively affected by gastrointestinal parasites, but there was a genotype effect to the average daily weight gain (ADWG), where the SI and F1 genotypes presented higher values. The effects of genotype, time and genotype x time interaction were significant in weight and corporal score (CS) measurements. The BS lambs had the highest CS values throughout the experiment despite not presenting greater weight gain when compared to the SI and F1 breeds. There were also significant effects of time and genotype x time interaction for packed cell volume (PCV) and FAMACHA© score (FAM) and only the time effect was significant in the total number of eggs per gram (EPG) and total plasma protein (TPP). The MN lambs showed higher PCV values and unlike the other groups, presented a FAMACHA© score below 3 and PCV above 23% even having a higher EPG tendency, especially in the initial phase, indicating a possible higher resilience to infection caused by gastrointestinal parasites(AU)

O objetivo deste estudo foi comparar quatro grupos genéticos de ovinos: Santa Inês (SI), Somalis Brasileira (SB), Morada Nova (MN) e 1/2 Dorper - 1/2 Morada Nova (F1) quanto às características de crescimento e de infecção parasitária. Trinta e três cordeiros machos, de mesma idade, nascidos de parto simples e submetidos às mesmas condições de manejo pré-desmame foram utilizados no experimento. Após o desmame, os animais foram alojados em piquetes de capim Panicum maximum cv. Tanzânia, em um sistema rotativo de pastejo sob um delineamento inteiramente ao acaso. Os resultados mostraram que, ao longo do experimento, o desempenho ponderal dos quatro grupos genéticos foi negativamente afetado pela infecção por endoparasitas, mas o efeito de genótipo foi significativo para ganho de peso médio diário, e os grupos SI e F1 apresentaram melhores médias. O efeito do genótipo, tempo e interação genótipo x tempo foi significativo para o peso e o escore corporal. Os cordeiros da raça SB apresentaram maiores escores corporais ao longo do experimento, apesar do menor ganho de peso quando comparados aos dos grupos SI e F1. Houve efeito significativo do tempo e da interação genótipo x tempo para o hematócrito e para o grau FAMACHA©. Somente efeito do tempo foi verificado para as características OPG e proteína plasmática total. Os cordeiros da raça Morada Nova, ao contrário dos demais grupos, mantiveram o grau FAMACHA© inferior a 3 e hematócrito inferior a 23%, mesmo com tendência de maior OPG, principalmente na fase inicial, indicando uma possível maior capacidade de adaptação à infecção por endoparasitas(AU)

Performance and parasite control of different genetic groups of lambs finished in irrigated pasture / Desempenho e controle parasitário de diferentes grupos genéticos de cordeiros terminados em pastagem irrigada

The aim of this study was to compare the following four genetic groups of hair sheep: Santa Inês (SI), Morada Nova (MN), Brazilian Somali (BS), and the F1 1/2Dorper x 1/2Morada Nova crossbreed on traits related to growth and parasitic infection. Thirty-three male lambs of the same age and of simple birth, under the same pre-weaning management conditions were used in the experiment. After weaning the animals were housed in a completely randomized design in paddocks made of Panicum maximum cv. Tanzania. Along the course of the research, the performance of the four groups of sheep was observed to be negatively affected by gastrointestinal parasites, but there was a genotype effect to the average daily weight gain (ADWG), where the SI and F1 genotypes presented higher values. The effects of genotype, time and genotype x time interaction were significant in weight and corporal score (CS) measurements. The BS lambs had the highest CS values throughout the experiment despite not presenting greater weight gain when compared to the SI and F1 breeds. There were also significant effects of time and genotype x time interaction for packed cell volume (PCV) and FAMACHA© score (FAM) and only the time effect was significant in the total number of eggs per gram (EPG) and total plasma protein (TPP). The MN lambs showed higher PCV values and unlike the other groups, presented a FAMACHA© score below 3 and PCV above 23% even having a higher EPG tendency, especially in the initial phase, indicating a possible higher resilience to infection caused by gastrointestinal parasites.

O objetivo deste estudo foi comparar quatro grupos genéticos de ovinos: Santa Inês (SI), Somalis Brasileira (SB), Morada Nova (MN) e 1/2 Dorper - 1/2 Morada Nova (F1) quanto às características de crescimento e de infecção parasitária. Trinta e três cordeiros machos, de mesma idade, nascidos de parto simples e submetidos às mesmas condições de manejo pré-desmame foram utilizados no experimento. Após o desmame, os animais foram alojados em piquetes de capim Panicum maximum cv. Tanzânia, em um sistema rotativo de pastejo sob um delineamento inteiramente ao acaso. Os resultados mostraram que, ao longo do experimento, o desempenho ponderal dos quatro grupos genéticos foi negativamente afetado pela infecção por endoparasitas, mas o efeito de genótipo foi significativo para ganho de peso médio diário, e os grupos SI e F1 apresentaram melhores médias. O efeito do genótipo, tempo e interação genótipo x tempo foi significativo para o peso e o escore corporal. Os cordeiros da raça SB apresentaram maiores escores corporais ao longo do experimento, apesar do menor ganho de peso quando comparados aos dos grupos SI e F1. Houve efeito significativo do tempo e da interação genótipo x tempo para o hematócrito e para o grau FAMACHA©. Somente efeito do tempo foi verificado para as características OPG e proteína plasmática total. Os cordeiros da raça Morada Nova, ao contrário dos demais grupos, mantiveram o grau FAMACHA© inferior a 3 e hematócrito inferior a 23%, mesmo com tendência de maior OPG, principalmente na fase inicial, indicando uma possível maior capacidade de adaptação à infecção por endoparasitas.

Plantas medicinais conhecidas por especialistas locais de uma comunidade rural maranhense / Known medicinal plants by local experts in a rural community in Maranhão

RESUMO Este trabalho objetivou realizar o inventário das plantas medicinais de uma comunidade rural maranhense, analisando o valor de uso e a importância relativa das espécies, assim como o consenso dos informantes quanto às doenças tratadas. Foram realizadas 50 entrevistas semiestruturadas utilizando-se o método de amostragem “bola-de-neve” e usou-se a técnica “turnê-guiada” para coletar as espécies citadas. Os especialistas locais citaram 63 espécies pertencentes a 44 famílias, das quais Fabaceae, Apocynaceae, Lamiaceae e Myrtaceae apresentaram o maior número de espécies. A planta que obteve maior valor de uso foi Morinda citrifolia L.. Himatanthus drasticus (Müll.Arg.) Woodson foi a espécie que apresentou maior importância relativa. Dentre os sistemas corporais tratados com os recursos vegetais, os que obtiveram maior consenso entre os informantes foram os relacionados às doenças do estômago, esôfago e duodeno e infecções virais caracterizadas por lesões na pele (ambas com FCI = 0,83). Em virtude da riqueza de conhecimento sobre plantas medicinais em um Estado do Brasil onde o sistema de saúde é bastante precário e onde os estudos de cunho etnobotânico ainda são escassos, sugere-se que novos estudos etnobotânicos sejam realizados afim de evidenciar a dinâmica de conhecimento local e suas implicações ecológicas e sociais.

ABSTRACT This study aimed to conduct an inventory of medicinal plants in a rural community in Maranhão, analyzing the use value and the relative importance of species as well as the consensus of the informants about the treated diseases. Fifty semi-structured interviews were conducted using the sampling method “snow-ball” and the technique “guided tour” was employed in order to collect the species mentioned. Local experts mentioned 63 species belonging to 44 families, from which Fabaceae, Apocynaceae, Lamiaceae and Myrtaceae represented the highest number of species. The plant which had the highest use value was the Morinda citrifolia L.. The Himatanthus drasticus (Müll.Arg.) Woodson was the species with the highest relative importance. Among the body systems treated with plant resources, those who had the most consensus among the informants were related to stomach, esophagus and duodenum diseases and viral infections characterized by skin lesions (both ICF = 0.83). Because of the abundance of knowledge about medicinal plants in this state of Brazil, where the health system is quite precarious and where the nature of ethnobotanical studies are still scarce, it is suggested that new ethnobotanical studies are conducted in order to show the dynamics of local knowledge and its ecological and social implications.

Single nucleotide polymorphisms in candidate genes associated with gastrointestinal nematode infection in goats.

Cytokines are small cell-signaling proteins that play an important role in the immune system, participating in intracellular communication. Four candidate genes of the cytokine family (IL2, IL4, IL13, and IFNG) were selected to identify Single Nucleotide Polymorphisms (SNPs) that might be associated with resistance to gastrointestinal endoparasites in goats. A population of 229 goats, F2 offspring from an F1 intercross was produced by crossing pure Saanen goats, considered as susceptible to gastrointestinal endoparasites, with pure Anglo-Nubian goats, considered resistant. Blood was collected for DNA extraction and fecal samples were also collected for parasite egg count. Polymorphisms were prospected by sequencing animals with extreme phenotype for fecal egg count (FEC) distribution. The association between SNPs and phenotype was determined by using the Fisher exact test with correction for multiple tests. Three of the 10 SNPs were identified as significant (P ≤ 0.03). They were found in intron 1 of IL2 (ENSBTA00000020883), intron 3 of IL13 (ENSBTA00000015953) and exon 3 of IFNG (ENSBTA00000012529), suggesting an association between them and gastrointestinal endoparasite resistance. Further studies will help describe the effects of these markers accurately before implementing them in marker assisted selection. This study is the pioneer in describing such associations in goats.

Response of resistant and susceptible Brazilian Somalis crossbreed sheep naturally infected by Haemonchus contortus.

This study was carried out to evaluate the performance of Brazilian Somalis sheep to natural infections by gastrointestinal nematodes. During 98 days, 75 weaned sheep, initially 3-4 months old, were kept on the same pasture and evaluated. Fecal and blood samples were collected for parasitological and hematological exams. After this period, the eight most resistant and the eight most susceptible animals were selected based on their individual averages of nematode fecal egg counts and were slaughtered for worm burden determination and nematodes identification. Abomasum and abomasum lymph nodes were also recovered for gene expression analysis. The animals selected as resistant had lower fecal egg counts during experimental period and smaller worm burdens than the susceptible ones (P < 0.05). The genus Haemonchus, followed by Trischostrongylus and Oesophagostomum, were identified in composite cultures. Haemonchus contortus was the specie identified in the abomasum. Packed cell volume and total plasma protein means were higher in the resistant group (27.2% and 6.1 g/dL) than in the susceptible one (22.5% and 5.3 g/dL), respectively. Regarding cytokine gene expression, IL-4 (P < 0.05) was up-regulated in the abomasum of resistant animals and TNF-α (P < 0.03) and IFN-γ (P < 0.03) in susceptible ones. In abomasum lymph nodes, IL-4 (P < 0.04) and IL-13 (P < 0.05) were up-regulated in the resistant animals and IFN-γ in the susceptible one (P < 0.01). This work provides further evidence that, within a given animal breed, individuals have different responses when infected by gastrointestinal nematodes. Resistant animals who responded more quickly and efficiently to these infections activated a TH2-type response.

Genotype effect on carcass and meat quality of lambs finished in irrigated pastures in the semiarid Northeastern Brazil / Efeito do genótipo sobre características de carcaça e qualidade da carne de cordeiros terminados em pastagem irrigada no semiárido do Nordeste do Brasil

The aim of this study was to compare four genetic groups of sheep on the carcass and meat quality traits. Thirty-three contemporary and unrelated male lambs, all of single birth were used in the experiment, being thirteen from the Santa Inês (SI) breed, seven from the Brazilian Somali breed (BS), six from the Morada Nova (MN) breed and seven from the ½ Dorper - ½ Morada Nova (F1) crossbreed. The genotypes SI, BS and F1 presented similar performances in relation to hot and cold carcass weights, which values were 10.76±0.53kg and 10.46±0.52kg for SI, 9.20±0.73kg and 8.99±0.71kg for BS, and 9.35±0.73kg and 9.13±0.71kg for F1, respectively. The BS had a better hot carcass yield (47.10±0.88%) and cold carcass yield (46.00±0.87%). Better carcass conformation was observed in SI and F1 (2.73±0.12 and 2.50±0.17, respectively) while the BS presented a better finishing (3.29±0.20). The average for the rib eye area (REA) was 9.94±0.49cm², 8.66±0.67cm², 7.18±0.72cm² and 9.8±0.67cm², and for the carcass compactness index (CCI) it was 0.17±0.01kg/cm, 0.17±0.01kg/cm, 0.11±0.01kg/cm and 0.16±0,01kg/cm, for SI, SB, MN and F1, respectively. There were no significant differences between SI, BS and F1 regarding REA and CCI. Although, in general, the MN presented a relatively lower performance than the other genotypes, this breed had similar carcass yields and fat thickness when compared to SI and F1 and similar conformation and REA in comparison to the BS. Regarding meat quality, no differences were observed between genotypes, except for redness and cooking losses. It is concluded that no one group had a higher or lower performance in all traits analyzed. Moreover, for the management conditions employed in this study, there was evidence of greater specialization in meat production for genotypes SI, BS and F1 when compared to MN, although there are no substantial differences between the four groups regarding meat quality.(AU)

O objetivo deste estudo foi comparar quatro grupos genéticos de ovinos quanto às características de carcaça e de qualidade da carne. Trinta e três cordeiros, contemporâneos, nascidos de parto simples e sem relação de parentesco, foram utilizados no experimento, sendo 13 da raça Santa Inês (SI), sete da raça Somalis Brasileira (SB), seis da raça Morada Nova (MN) e sete ½ Dorper - ½ Morada Nova (F1). Os genótipos SI, SB e F1 apresentaram desempenhos similares quanto ao peso das carcaças quente e fria, cujos valores foram 10,76±0,53kg e 10,46±0,52kg para o SI, 9,20±0,73kg e 8,99±0,71kg para o SB, 9,35±0,73kg e 9,13±0,71kg para o F1, respectivamente. A raça SB apresentou melhor rendimento de carcaça quente (47,10 ±0,88%) e de carcaça fria (46,00±0,87%). Os genótipos SI e F1 apresentaram melhores conformações (2,73±0,12 e 2,50±0,17, respectivamente), enquanto o SB apresentou melhor acabamento (3,29±0,20). As médias para área de olho de lombo (AOL) foram 9,94±0,49cm², 8,66±0,67cm², 7,18±0,72cm² e 9,8±0,67cm² e para o índice de compacidade da carcaça (ICC) foram 0,17±0,01kg/cm, 0,17±0,01kg/cm, 0,11±0,01kg/cm e 0,16±0,01kg/cm, para SI, SB, MN e F1, respectivamente. Não houve diferença significativa entre os genótipos SI, SB e F1 quanto à AOL e ao ICC. Apesar de, no geral, a raça MN apresentar desempenho relativamente inferior aos demais genótipos quanto às características de carcaça analisadas, essa raça apresentou rendimentos de carcaça e espessura de gordura similar ao SI e F1 e conformação e AOL similar ao SB. Não houve um grupo superior ou inferior para todas as características analisadas. Por outro lado, para as condições de manejo empregadas neste estudo, evidenciou-se maior especialização para produção de carne dos genótipos SI, SB e F1 em relação ao grupo MN. Não houve diferenças importantes entre os diferentes genótipos quanto à qualidade da carne.(AU)

Genotype effect on carcass and meat quality of lambs finished in irrigated pastures in the semiarid Northeastern Brazil / Efeito do genótipo sobre características de carcaça e qualidade da carne de cordeiros terminados em pastagem irrigada no semiárido do Nordeste do Brasil

The aim of this study was to compare four genetic groups of sheep on the carcass and meat quality traits. Thirty-three contemporary and unrelated male lambs, all of single birth were used in the experiment, being thirteen from the Santa Inês (SI) breed, seven from the Brazilian Somali breed (BS), six from the Morada Nova (MN) breed and seven from the ½ Dorper - ½ Morada Nova (F1) crossbreed. The genotypes SI, BS and F1 presented similar performances in relation to hot and cold carcass weights, which values were 10.76±0.53kg and 10.46±0.52kg for SI, 9.20±0.73kg and 8.99±0.71kg for BS, and 9.35±0.73kg and 9.13±0.71kg for F1, respectively. The BS had a better hot carcass yield (47.10±0.88%) and cold carcass yield (46.00±0.87%). Better carcass conformation was observed in SI and F1 (2.73±0.12 and 2.50±0.17, respectively) while the BS presented a better finishing (3.29±0.20). The average for the rib eye area (REA) was 9.94±0.49cm², 8.66±0.67cm², 7.18±0.72cm² and 9.8±0.67cm², and for the carcass compactness index (CCI) it was 0.17±0.01kg/cm, 0.17±0.01kg/cm, 0.11±0.01kg/cm and 0.16±0,01kg/cm, for SI, SB, MN and F1, respectively. There were no significant differences between SI, BS and F1 regarding REA and CCI. Although, in general, the MN presented a relatively lower performance than the other genotypes, this breed had similar carcass yields and fat thickness when compared to SI and F1 and similar conformation and REA in comparison to the BS. Regarding meat quality, no differences were observed between genotypes, except for redness and cooking losses. It is concluded that no one group had a higher or lower performance in all traits analyzed. Moreover, for the management conditions employed in this study, there was evidence of greater specialization in meat production for genotypes SI, BS and F1 when compared to MN, although there are no substantial differences between the four groups regarding meat quality.

O objetivo deste estudo foi comparar quatro grupos genéticos de ovinos quanto às características de carcaça e de qualidade da carne. Trinta e três cordeiros, contemporâneos, nascidos de parto simples e sem relação de parentesco, foram utilizados no experimento, sendo 13 da raça Santa Inês (SI), sete da raça Somalis Brasileira (SB), seis da raça Morada Nova (MN) e sete ½ Dorper - ½ Morada Nova (F1). Os genótipos SI, SB e F1 apresentaram desempenhos similares quanto ao peso das carcaças quente e fria, cujos valores foram 10,76±0,53kg e 10,46±0,52kg para o SI, 9,20±0,73kg e 8,99±0,71kg para o SB, 9,35±0,73kg e 9,13±0,71kg para o F1, respectivamente. A raça SB apresentou melhor rendimento de carcaça quente (47,10 ±0,88%) e de carcaça fria (46,00±0,87%). Os genótipos SI e F1 apresentaram melhores conformações (2,73±0,12 e 2,50±0,17, respectivamente), enquanto o SB apresentou melhor acabamento (3,29±0,20). As médias para área de olho de lombo (AOL) foram 9,94±0,49cm², 8,66±0,67cm², 7,18±0,72cm² e 9,8±0,67cm² e para o índice de compacidade da carcaça (ICC) foram 0,17±0,01kg/cm, 0,17±0,01kg/cm, 0,11±0,01kg/cm e 0,16±0,01kg/cm, para SI, SB, MN e F1, respectivamente. Não houve diferença significativa entre os genótipos SI, SB e F1 quanto à AOL e ao ICC. Apesar de, no geral, a raça MN apresentar desempenho relativamente inferior aos demais genótipos quanto às características de carcaça analisadas, essa raça apresentou rendimentos de carcaça e espessura de gordura similar ao SI e F1 e conformação e AOL similar ao SB. Não houve um grupo superior ou inferior para todas as características analisadas. Por outro lado, para as condições de manejo empregadas neste estudo, evidenciou-se maior especialização para produção de carne dos genótipos SI, SB e F1 em relação ao grupo MN. Não houve diferenças importantes entre os diferentes genótipos quanto à qualidade da carne.

Effects of Mimosa tenuiflora on larval establishment of Haemonchus contortus in sheep.

Anthelmintic resistance has limited the ability to control the gastrointestinal nematodes of small ruminants and has therefore awakened an interest in the study of tanniferous plants as a source of anthelmintics. This study was carried out to evaluate the effect of Mimosa tenuiflora intake, a tanniferous plant that is fed to small ruminants in northeastern Brazil, on the larval establishment of Haemonchus contortus in sheep. In this experiment, 18 nematode-free sheep were divided into three groups (n=6) according to live weight. Group 1 was fed M. tenuiflora leaves; Group 2 was fed M. tenuiflora stems; Group 3 served as the control group and was fed Cynodon dactylon, a plant with low levels of tannins. The animals consumed the plants for 13 days (Day -7 to Day 5). On Day 0, the sheep were experimentally infected with 4500 third-stage H. contortus each. Five days after infection (Day 5), the sheep were slaughtered to count the worm burden and perform a histological analysis of the abomasum. The daily plant intake and the live weight gain of the animals were recorded. The groups that ingested M. tenuiflora leaves and stems consumed less dry matter than did those that ingested C. dactylon (P<0.05). The consumption of M. tenuiflora leaves did not reduce the L3 establishment of H. contortus compared to the control (P>0.05). The intake of M. tenuiflora stems tended toward decreasing larval establishment, but the reduction was not significant (P>0.05). No significant differences were observed in the mucosal cellular response and live weight gain among the groups. These data demonstrated that, with the protocol used, M. tenuiflora has no effect on larval establishment of H. contortus in sheep.

Suppression of SERK gene expression affects fungus tolerance and somatic embryogenesis in transgenic lettuce.

The Somatic embryogenesis receptor-like kinase (SERK) gene plays an important role in plant somatic and zygotic embryogenesis induction. The gene encodes an LRR-containing receptor-like kinase protein. Studies have been carried out focusing on different aspects of its function, but definitive conclusions on its role are far from being reached. SERK expression is generally detected in cells in which somatic or zygotic embryogenesis has been triggered. Transgenic lettuce lines were produced to silence the endogenous SERK gene using antisense RNA. The average number of seeds per flower in the R(1) and R(2) generations was similar for both transgenic and non-transgenic lines. However, a reduction in the number of viable grained seeds was observed in four studied transgenic lines. Endogenous SERK expression analysis revealed the absence of detectable LsSERK gene transcripts in three transgenic lines, which presented a reduction in their ability to form in vitro somatic embryonic structures. In addition, transgenic lines showed enhanced susceptibility to the pathogenic fungus Sclerotinia sclerotiorum, when compared to control plants. The results support the idea that SERK genes might not only be involved in plant growth and development, but probably also in a general mechanism of biotic and abiotic stress perception.

Anthelmintic activity of Cocos nucifera L. against sheep gastrointestinal nematodes.

The development of anthelmintic resistance has made the search for alternatives to control gastrointestinal nematodes of small ruminants imperative. Among these alternatives are several medicinal plants traditionally used as anthelmintics. This work evaluated the efficacy of Cocos nucifera fruit on sheep gastrointestinal parasites. The ethyl acetate extract obtained from the liquid of green coconut husk fiber (LGCHF) was submitted to in vitro and in vivo tests. The in vitro assay was based on egg hatching (EHT) and larval development tests (LDT) with Haemonchus contortus. The concentrations tested in the EHT were 0.31, 0.62, 1.25, 2.5 and 5 mg ml(-1), while in the LDT they were 5, 10, 20, 40 and 80 mg ml(-1). The in vivo assay was a controlled test. In this experiment, 18 sheep infected with gastrointestinal nematodes were divided into three groups (n=6), with the following doses administered: G1-400 mg kg(-1) LGCHF ethyl acetate extract, G2-0.2 mg kg(-1) moxidectin (Cydectin) and G3-3% DMSO. The worm burden was analyzed. The results of the in vitro and in vivo tests were submitted to ANOVA and analyzed by the Tukey and Kruskal-Wallis tests, respectively. The extract efficacy in the EHT and LDT, at the highest concentrations tested, was 100% on egg hatching and 99.77% on larval development. The parameters evaluated in the controlled test were not statistically different, showing that despite the significant results of the in vitro tests, the LGCHF ethyl acetate extract showed no activity against sheep gastrointestinal nematodes.

Ocorrência de ctenocephalides felis felis (bouché, 1835) em caprinos no rio grande do norte, brasil

ABSTRACT First report of Ctenocephalides felis felis (Siphonaptera) infesting young goats (12.3%), from rural properties, in the town of Mossoro, a semi-arid region of Rio Grande do Norte State, Brazil.

RESUMO Primeiro registro de Siphonaptera da espécie Ctenocephalides felis felis parasitando caprinos jovens (12,3%), procedentes de propriedades rurais, no Município de Mossoró, na região semiárida do Estado do Rio Grande do Norte, Brasil.

Anthelmintic activity of Lippia sidoides essential oil on sheep gastrointestinal nematodes.

Medicinal plants have been investigated for their anthelmintic properties and shown to be effective against eggs and larvae of gastrointestinal nematodes. The aim of this study was to evaluate the efficacy of the Lippia sidoides essential oil (LsEO) on sheep gastrointestinal nematodes. Initially, 44 naturally infected sheep were divided and treated with 200 microg kg(-1) ivermectin and 230 and 283 mg kg(-1) LsEO, respectively, plus the control. Fecal samples were collected from each animal to determine epg at 7, 14 and 21 days after treatment. In another test, 21 sheep were distributed and treated with 200 microg kg(-1) ivermectin, 283 mg kg(-1) LsEO and the control, respectively. Seven days after treatment, they were euthanized and necropsied to count and identify the nematodes from the abomasum, small and large intestines. In the first test, the efficacy of 230 and 283 mg kg(-1) LsEO and ivermectin was 38%, 45.9% and 40.2%, respectively, 7 days after treatment, and 30%, 54% and 39.6%, respectively, 14 days after treatment. In the second experiment, the respective efficacy of 283 mg kg(-1) LsEO and ivermectin was 56.9% and 34.4% against Haemonchus spp., and 39.3% and 63.6% against Trichostrongylus spp.

Anthelmintic efficacy of neem (Azadirachta indica A. Juss) and the homeopathic product Fator Vermes in Morada Nova sheep.

Gastrointestinal nematodes are becoming increasingly resistant to the commercial products used to control them. The cost of routine vermifuge applications on herds and the problem of residues in animal products and the environment have prompted research on the anthelmintic activity of plant extracts. This work examines the anthelmintic action of neem and the homeopathic product Fator Vermes in sheep kept in a pasture for 18 months. Forty sheep of the Morada Nova breed were divided into four treatments and the control, according to the EPG. During the experiment, each animal received 100 g/day of shredded corn and did not receive protein supplementation. In treatment 1 (control), the animals received only shredded corn. Treatment 2 received 1.6 g/(animal day) of the homeopathic product mixed with the shredded corn, and treatments 3, 4 and 5 received, respectively, 12.5, 25.0 and 37.5 g/(animal day) of dried Azadirachta indica leaves mixed with the shredded corn. The neem was administered for alternating 15-day periods and the homeopathic product daily for 18 months. There were 39 fortnightly fecal collections made to count the EPG, and fecal cultures were performed monthly. The following genera, in percentage, were identified: Haemonchus: 65.58+/-3.27, Trichostrongylus: 15.92+/-7.38 and Oesophagostomum: 18.50+/-6.22. The treatments evaluated were not effective in controlling gastrointestinal nematodes (P>0.05), whose mean log(10) counts (EPG +1) and standard errors for treatments 1-5 were respectively 3.55+/-0.28; 3.48+/-0.31; 3.90+/-0.29; 2.78+/-0.29 and 3.48+/-0.30. A significant effect (P<0.0001) was observed of the periods of the year when the 39 collections occurred. Because of the diet deficient in raw protein, the sheep had higher average EPG counts, for all the treatments, at the end of the dry season, and the opposite occurred in the middle of the rainy season.

Anthelmintic activity of Croton zehntneri and Lippia sidoides essential oils.

Because of the development of anthelmintic resistant populations, the search for new drugs is essential to maintain the productivity of small ruminants. The aim of this study was to evaluate the anthelmintic activity of Croton zehntneri and Lippia sidoides essential oils and their major constituents, anethole and thymol. The effects of these oils and their constituents were determined by in vitro assays with the eggs and larvae of the sheep gastrointestinal nematode Haemonchus contortus. The two essential oils were evaluated on intestinal nematodes of mice at 800 mg kg(-1) dose. In the last experiment, the mice were treated with larger doses of L. sidoides, 1200 and 1600 mg kg(-1). The essential oils and their constituents prevented more than 98% of the H. contortus eggs from hatching at a concentration of 1.25 mg ml(-1) and inhibited more than 90% of H. contortus larval development at a concentration of 10 mg ml(-1). At a concentration of 800 mg kg(-1), the two essential oils were 46.3% and 11.64% effective against Syphacia obvelata and Aspiculuris tetraptera. At 1200 and 1600 mg kg(-1), L. sidoides essential oil's efficacy on the mouse worm burden was 57.6% and 68.9%, respectively. The fact that L. sidoides essential oil was almost 70% effective against mouse intestinal nematodes indicates it should be evaluated against gastrointestinal nematodes of sheep and goats.

Ovicidal and larvicidal activity in vitro of Spigelia anthelmia Linn. extracts on Haemonchus contortus.

The rapid development of anthelmintic resistance, associated with the high cost of the available anthelmintic drugs, had limited the success of gastrointestinal nematodiasis control in sheep and goats and thus awakened interest in the study of medicinal plants as alternative sources of anthelmintics. Spigelia anthelmia extracts obtained with hexane, chloroform, ethyl acetate or methanol, were tested on Haemonchus contortus eggs and larvae via egg hatch and larval development tests. The extracts were evaluated at five concentrations: 3.1, 6.2, 12.5, 25.0 and 50.0 mg ml(-1). At 50.0 mg ml(-1), the ethyl acetate extract inhibited 100% of the egg hatching and 81.2% of the larval development. In a similar way the methanolic extract inhibited 97.4% of the egg hatching and 84.4% of larval development. These results suggest that utilization of S. anthelmia extracts may be useful in the control of sheep and goats gastrointestinal nematodes.

Development of Eimeria ninakohlyakimovae Yakimoff & Rastegaieff, 1930 emend. Levine, 1961 in experimentally infected goats (Capra hircus).

The endogenous development and prepatent and patent periods of Eimeria ninakohlyakimovae were studied in 43 1-3-wk-old coccidia-free kids inoculated with 5.0 x 10(4), 1.5 x 10(5), 2.0 x 10(5), or 9.0 x 10(5) sporulated oocysts/kg. Twenty-five kids were killed at 24- or 48-hr intervals, 2-18 days after inoculation (DAI). Two generations of meronts, gamonts, gametes, and oocysts were found in sections stained with hematoxylin and eosin and examined using under light microscopy. The first generation of meronts developed in the endothelium of the lacteals, in the lamina propria, and in the lymphatic vessels of the ileum submucosa. Mature, first-generation meronts, 165.5 x 123.6 microm, were first found 10 DAI. Second-generation merogony developed in the crypt epithelial cells of the cecum and colon; mature meronts, 16.8 x 11.6 microm, were first seen 12 DAI. Gametogenesis occurred in the cecum and colon epithelium; mature microgamonts (16.1 x 13.0 microm), microgametes, macrogametes (14.7 x 12.5 microm), and oocysts (18.3 x 13.3 microm) were seen at 13 DAI. The course of the infection was followed in 18 kids examined every day until 24 DAI. The prepatent period was 14.7 (13-17) days and the patent period 6.8 (4-10) days. The sporulation time at 30 C, with constant aeration, was 2-3 days.

Ultrastructure of endogenous stages of Eimeria ninakohlyakimovae Yakimoff & Rastegaieff, 1930 Emend. Levine, 1961 in experimentally infected goat.

The ultrastructure of endogenous stages of Eimeria ninakohlyakimovae was observed in epithelial cells of cecum and colon crypts from a goat experimentally infected with 2.0 x 10(5) oocysts/kg. The secondary meronts developed above the nucleus of the host cell. The nucleus first divides and merozoites then form on the surface of multinucleated meronts. Free merozoites in the parasitophorous vacuole present a conoid, double membrane, one pair of rhoptries, micronemes, micropore, anterior and posterior polar ring, a nucleus with a nucleolus and peripheral chromatin. The microgamonts are located below the nucleus of the host cell and contain several nuclei at the periphery of the parasite. The microgametes consist of a body, a nucleus, three flagella and mitochondria. The macrogamonts develop below the nucleus of the host cell and have a large nucleus with a prominent nucleolus. The macrogametes contain a nucleus, wall-forming bodies of type I and type II. The young oocysts present a wall containing two layers and a sporont.

Outbreak of cryptosporidiosis in dairy goats in Brazil.

An outbreak of cryptosporidiosis is reported among 22 suckling kids aged one to two weeks which were maintained for experimental purposes at the veterinary hospital of the University of Minas Gerais. They were divided into three groups. Group A consisted of 10 animals with acute diarrhoea; initially their faeces were pasty but later they were excreted in watery streams. The animals were treated with gentamicin and fluid therapy but did not respond to treatment and died within a week. Postmortem examination revealed liquid intestinal contents, enlarged mesenteric lymph nodes, and hyperaemia and haemorrhage were observed in the final third of the small intestine of some of the animals. Of the 10 animals in group B four had diarrhoea and six were normal; postmortem examination showed that the macroscopic changes were similar to those observed in group A. Three of the animals with diarrhoea had a massive Cryptosporidium infection in the final third of the small intestine, caecum and colon. Four of the six normal animals had a moderate Cryptosporidium infection in the same organs. The two kids in group C died; they had pasty faeces, and there were many Cryptosporidium oocysts in the faecal smears.

Haemonchus contortus resistance to ivermectin and netobimin in Brazilian sheep.

Suffolk, Texel, Hampshire Down and Ile de France sheep from the municipalities of Porto Amazonas, Piraquara and Araucaria in the State of Paraná, and Bagé in the State of Rio Grande do Sul were brought to Sobral, State of Ceará, to be used in a cross-breeding project. On arrival they had clinical signs of nematode parasitosis, and one Suffolk female died. The animals were treated orally with ivermectin (0.2 mg kg-1) and fifteen days later with netobimin (20.0 mg kg-1). Neither drug reduced the egg counts (measured in eggs per gram, EPG) significantly, and this suggested that the nematodes in the sheep were resistant to the anthelmintics used. Haemonchus contortus was the species involved. The egg counts were reduced after oral treatment with trichlorfon (100.0 mg kg-1). Haemonchus contortus larvae obtained from these animals before trichlorfon treatment and passaged through two nematode-free sheep were used in a further experiment. Twenty 6- to 9-month-old nematode-free lambs were infected with the H. contortus larvae (10,000 per animal) and after the infection was confirmed, were randomly divided into four groups of five animals. Group I was orally treated with ivermectin at 0.2 mg kg-1, Group II with oral netobimin at 20.0 mg kg-1, Group III with oral trichlorfon at 100.0 mg kg-1 and Group IV was a non-treated control. Egg counts and faecal cultures were taken before dosing on the day of treatment and seven days later when all animals were necropsied and the nematodes were collected from the abomasa and counted.(ABSTRACT TRUNCATED AT 250 WORDS)