Potential of the Blue Calm® food supplement in the treatment of alcohol withdrawal-induced anxiety in adult zebrafish (Danio rerio).

Alcohol use disorder (AUD) is characterized by a set of behavioral, cognitive, nutritional, and physiological phenomena derived from the uncontrolled use of alcoholic beverages. There are cases in which AUD is associated with anxiety disorder, and when untreated, it requires careful pharmacotherapy. Blue Calm® (BC) is a food supplement indicated to aid restorative sleep, which has traces of medicinal plant extracts, as well as myo-inositol, magnesium bisglycinate, taurine, and L-tryptophan as its main chemical constituents. In this context, this study aimed to evaluate the potential of the BC in the treatment alcohol withdrawal-induced anxiety in adult zebrafish (aZF). Initially, BC was submitted to antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl radical. Subsequently, the aZF (n = 6/group) were treated with BC (0.1 or 1 or 10 mg/mL; 20 µL; p.o.), and the sedative effect and acute toxicity (96 h) were evaluated. Then, the anxiolytic-like effect and the possible GABAergic mechanism were analyzed through the Light & Dark Test. Finally, BC action was evaluated for treating alcohol withdrawal-induced anxiety in aZF. Molecular docking was performed to evaluate the interaction of the major chemical constituents of BC with the GABAA receptor. BC showed antioxidant potential, a sedative effect, was not toxic, and all doses of BC had an anxiolytic-like effect and showed potential for the treatment of alcohol withdrawal-induced anxiety in aZF. In addition to the anxiolytic action, the main chemical constituents of BC were confirmed in the molecular docking, thus suggesting that BC is an anxiolytic that modulates the GABAergic system and has pharmacological potential for the treatment of alcohol withdrawal-induced anxiety.

Understanding the Seasonal Effect of Metabolite Production in Terminalia catappa L. Leaves through a Concatenated MS- and NMR-Based Metabolomics Approach.

Terminalia catappa L. (Combretaceae) is a medicinal plant that is part of the Brazilian biodiversity; this plant is popularly used for the treatment of a wide range of diseases. To better understand the chemical composition of T. catappa in different seasons, we conducted a thorough study using LC-MS and NMR data analysis techniques. The study helped obtain a chemical profile of the plant ethanolic extracts in different seasons of the year (spring, summer, autumn, and winter). The dereplication of LC-HRMS data allowed the annotation of 90 compounds in the extracts of T. catappa (hydrolyzable tannins, ellagic acid derivatives, and glycosylated flavonoids). Triterpenes and C-glycosyl flavones were the compounds that significantly contributed to differences observed between T. catappa plant samples harvested in autumn/winter and spring, respectively. The variations observed in the compound composition of the plant leaves may be related to processes induced by environmental stress and leaf development. Data fusion applied in the metabolomic profiling study allowed us to identify metabolites with greater confidence, and provided a better understanding regarding the production of specialized metabolites in T. catappa leaves under different environmental conditions, which may be useful to establish appropriate quality criteria for the standardization of this medicinal plant.

Fear of cancer recurrence among Brazilian patients with cancer: Translation and cultural adaptation of FCR4/7 and FCRI-SF measures.

OBJECTIVE: Fear of cancer recurrence or progression (FCR) is considered one of the most common unmet needs among patients with cancer. This study sought to translate and evaluate the psychometric properties of the Fear of Cancer Recurrence scale (FCR4/7) and Fear of Cancer Recurrence Inventory-Short Form (FCRI-SF). METHODS: This study involved three phases: (1) translation and cultural adaptation of the FCR4/7 and FCRI-SF measures, (2) validity and reliability testing of the Portuguese version of these measures, and (3) examining patient's perceptions of these measures. Eligible patients were diagnosed with localized breast cancer, and patients with metastatic cancer. Descriptive analyses were collated, and psychometric analysis were conducted (confirmatory factor analysis). RESULTS: A total of 200 patients were recruited (100 patients with localized and 100 patients with metastatic cancer). A significant proportion of patients reported moderate to severe FCR (FCR7: 32.0% and FCRI-SF: 43.0%). Female gender, younger age and metastatic cancer were associated with higher levels of FCR. Psychometric analyses suggested that the Portuguese versions of the FCR4/7 and FCRI-SF were valid, unidimensional in nature, with acceptable reliability coefficients across all scales. In a sub-sample qualitative analysis (n = 75), most patients were satisfied with the relevance of both measures. CONCLUSION: Our findings suggest the Portuguese versions of the FCR4/7 and FCRI-SF are valid tools to assess FCR among patients with localized and metastatic cancer. Future research can now extend our understanding of FCR and assess this construct among Portuguese speaking patients, to guide the development of effective and targeted interventions for patients globally.

Saúde, trabalho e presença de fatores de risco cardiovascular em docentes de uma universidade pública / Health, work and presence of cardiovascular risk factors in professors at a public university

RESUMOObjetivo: identificara presença dos principais fatores de risco para doenças cardiovasculares em docentes de uma universidade pública. Método: Estudo transversal e de natureza quantitativa com docentes de uma Instituição de Ensino Superior a partir da avaliação e classificação de variáveis clínicas e sociodemográficas registradas pela aplicação de formulário estruturado e interpretadas segundo nível de significância (p<0,05) após apreciação no Statistical Package for the Social Sciencesversão 20.0. A coleta realizou-se de agosto de 2016 a julho de 2018. Resultados: Participaram 261 docentes.O IMC obteve resultados significativos quando associado à faixa etária de 34 a 44 anos (p=0,005), sexo masculino (p=0,050) e alteração na circunferência abdominal feminina (p=0,015), PA (p=0,0038), colesterol (p=0,052) e triglicerídeos (p=0,040) em algum momento da vida. Conclusão:Os fatores de risco cardiovascular apresentaram significativa relevância no público estudado e denota que o sexo, alterações do IMC, sedentarismo, dislipidemias, hipertensão e fatores estressores estão associados às doenças cardiovasculares.

Objective:To identify the presence of the main risk factors for cardiovascular diseases in professors at a public university. Method:Cross-sectional and quantitative study with professors of a Higher Education Institution based on the evaluation and classification of clinical and sociodemographic variables recorded by the application of a structured form and interpreted according to the level of significance (p<0.05) after analysis in the Statistical Package for the Social Sciences version 20.0. Data collection took place from August 2016 to July 2018. Results:261 professors participated. BMI obtained significant results when associated with the age group from 34 to 44 years (p=0.005), male sex (p=0.050) and change in female waist circumference (p=0.015), BP (p=0.0038), cholesterol (p=0.052) and triglycerides (p=0.040) at some point in life. Conclusion:Cardiovascular risk factors were significantly relevant in the population studied and showed that sex, changes in BMI, sedentary lifestyle, dyslipidemia, hypertension and stressors are associated with cardiovascular diseases.

Artificial Diets With Different Protein Levels for Rearing Spodoptera frugiperda (Lepidoptera: Noctuidae).

Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera: Noctuidae) is a pest of great economic importance which can feed on more than 300 plant species. As it is polyphagous, its host plants may have variable physical and chemical constitutions. This may influence larval development, as protein and carbohydrate levels are important factors for adequate biological development. The aim of this study was to evaluate insect developmental parameters as well as to compare the food consumption of S. frugiperda larvae reared using diets with different protein levels under laboratory conditions. Three artificial diet formulations were used: one typically used for routine laboratory rearing, based on bean, wheat germ and brewer's yeast (D1); one containing half the original amount of protein (D2), and the other with twice the original amount of protein (D3). The relative consumption rate (RCR), relative growth rate (RGR), and efficiency of conversion of ingested food (ECI) for S. frugiperda fourth instar larvae varied among diets. The protein present in the diet influenced the duration of larval and pupal periods and pupal weight, but did not affect larval survival, fecundity and longevity of adults. The different protein levels in the diets did not negatively influence population growth, so these three diet variations can be used for mass rearing in the laboratory. However, the influence of these diets on successive generations of the insect remains untested.

A Community of Practice of primary health care occupational therapists: Advancing practice-based knowledge.

INTRODUCTION: New contexts of practice demand that professionals engage in critical reflection to handle new situations and to create new knowledge that is responsive to professional practices situated in unique historical and social contexts. Community of Practice offers a framework for professions to reflect together on practice dilemmas and to generate practical solutions. METHODS: This paper presents a participatory action research project that traces the trajectory of a Community of Practice made up of seven occupational therapists working in primary health care and a researcher team, in Brazil. This study mapped the Community of Practice's trajectory between 2013 and 2017 through a group timeline analysis, which occurred gradually, in a collaborative mode. RESULTS: Three distinct phases in the trajectory of the development of the Community of Practice were identified: narrative perspectives were utilised as a means to identify dilemmas and difficulties in practice; the investigation of clients' needs and identification of issues was an ongoing process; and the generation of practice-based knowledge through the development of instruments to sustain clinical reasoning was a creative solution to practical dilemmas. CONCLUSION: Three main aspects were highlighted: the partnership between researchers and practitioners as a potential avenue for the production of knowledge relevant to professional practice; the negotiation of the dilemma of "putting practice into words" in the context of constantly changing local and global perspectives; and the investigation of situated practice as an important element that can strengthen, strain, resist or even modify hegemonic perspectives of knowledge production in our field.

Acute and Chronic Toxicity of Indoxacarb in Two Populations of Plutella xylostella (Lepidoptera: Plutellidae).

The diamondback moth, Plutella xylostella (Linnaeus, 1758), is one of the main pests of brassicas, and various insecticides, such as indoxacarb, are used to control it. However, frequent insecticide applications favor the selection of resistant individuals. Thus, the residual and sublethal effects of indoxacarb in two populations of P. xylostella, one collected in the field (FP) and one from the laboratory (LP), were evaluated and compared. The objective of this research was to investigate the toxicity of indoxacarb at residual and sublethal levels in a field population of P. xylostella from Brazil and a population from the laboratory. Leaf-dip bioassays showed high toxicity, with LC50 values after 48 h of 3.7 and 6.9 mg/liter for the LP and FP, respectively. Sublethal effects were indicated by significant reduction in the survival of larvae, pupae, and offspring. There was an increase in foliar consumption and a decrease in adult survival in the LP, and a decrease in fecundity in the FP. For the LP and FP population, the mean values for R0, rm, and λ for the control treatment were significantly higher than for the treatment groups (CL15 and CL25). Exposure of larvae to sublethal indoxacarb concentrations significantly reduced larval and pupal survival. Larval and pupal survival decreased as the indoxacarb concentration increased. Fecundity was significantly lower for the FP at LC15 (96.2 eggs per female) and LC25 (69.2 eggs per female) concentrations compared with the other treatments.

Acceptance and Commitment Therapy and Drug Use: A Systematic Review / Terapia de Aceitação e Compromisso e Uso de Drogas: Uma Revisão Sistemática / Terapia de Aceptación y Compromiso y Uso de Drogas: Una Revisión Sistemática

Abstract The harmful use of psychoactive substances has been considered one of the biggest public health issues. Several approaches are used for the treatment of disorders related to substance use in the face of the challenges imposed. This study aimed to identify the empirical works that used ACT for the treatment of substance use and their respective results regarding acceptance, feasibility, and reduction in the severity of dependence, as well as the results related to comorbidities and other aspects associated with drugs use by a systematic review of the literature on the subject, using the recommendations of the Preferred Report Items for Systematic Reviews and Meta-Analyses (PRISMA) guide and searches in PubMed, APA PsycNET, SciELO, ScienceDirect, Web of Science, VHL, and LILACS. In total, 17 studies were analyzed, allowing the observation of an increase in psychological flexibility, attenuation of treatment dropout rates, and a reduction in substance use in the analyzed interventions.

Resumo O uso prejudicial de substâncias psicoativas tem sido considerado um dos maiores problemas de saúde pública. Diversas abordagens são utilizadas para o tratamento dos transtornos relacionados ao uso de substâncias diante dos desafios impostos. O presente estudo teve por objetivo identificar os trabalhos empíricos que utilizaram a ACT para o tratamento do uso de substâncias e seus respectivos resultados no que diz respeito à aceitação, viabilidade e redução da gravidade da dependência, bem como nos resultados relacionados às comorbidades e demais aspectos associados ao uso através de uma revisão sistemática da literatura acerca do tema, utilizando-se das recomendações do guia Preferred Report Items for Systematic Reviews and Meta-Analyses (PRISMA) e buscas nas bases Pubmed, APA PsycNET, SciELO, ScienceDirect, Web of Science, BVS e LILACS. Foram analisados 17 estudos, permitindo observar aumento da flexibilidade psicológica, atenuação das taxas de abandono do tratamento e redução do consumo de substâncias nas intervenções analisadas.

Resumen El uso nocivo de sustancias psicoactivas se ha considerado como uno de los mayores problemas de salud pública. Se utilizan varios enfoques para el tratamiento de los trastornos relacionados con el consumo de sustancias frente a los desafíos impuestos. Este estudio tuvo como objetivo identificar los trabajos empíricos que utilizaron ACT para el tratamiento del consumo de sustancias y sus respectivos resultados en cuanto a la aceptación, viabilidad y reducción de la gravedad de la dependencia, así como los resultados relacionados con las comorbilidades y otros aspectos asociados al uso, desde una revisión sistemática de la literatura sobre el tema y utilizando las recomendaciones de la guía Preferred Report Items for Systematic Reviews and Meta-Analyzes (PRISMA) y búsquedas en PubMed, APA PsycNET, SciELO, ScienceDirect, Web of Science, VHL y LILACS. Se analizaron 17 estudios, lo que permitió observar un aumento de la flexibilidad psicológica, una atenuación de las tasas de abandono del tratamiento y una reducción del consumo de sustancias en las intervenciones analizadas.

Temperature-dependent functional response of Euborellia annulipes (Dermaptera: Anisolabididae) preying on Plutella xylostella (Lepidoptera: Plutellidae) larvae.

Temperature mediates trophic interactions, including relationships between insect pests and predators, and functional response studies are often used to determine the suitability of predators as biocontrol agents. We investigated the effects of temperature on the functional response of Euborellia annulipes (Lucas) (Dermaptera: Anisolabididae) preying on Plutella xylostella (L.) (Lepidoptera: Plutellidae) larvae. Predation rate, type of functional response, attack rate (a'), handling time (Th), and maximum predation rate (T/Th) of the predator were estimated using seven prey densities and three thermal conditions. The functional response of E. annulipes to P. xylostella was temperature-dependent, type III under the lower temperatur (18°C and 25 °C) , and type II at 32 °C. We observed increasing values of a' in 25 °C and 32 °C, decreasing values of Th and highest T/Th as the thermal condition increased. Our findings suggest that E. annulipes could be effective to control P. xylostella under different thermal conditions, however its predation behavior changes according to temperature variation.

Peanut skin polyphenols inhibit toxicity induced by advanced glycation end-products in RAW264.7 macrophages.

This is the first work to use a polyphenolic fraction derived from peanut skin to attenuate the toxicity induced by advanced glycation-end products (AGEs) in RAW264.7 macrophages. The RAW264.7 cells were stimulated by AGEs using the bovine serum albumin-fructose (BSA-FRU), bovine serum albumin-methylglyoxal (BSA-MGO) and arginine-methylglyoxal (ARG-MGO) models. The AGEs increased considerably the levels of reactive oxygen species and the gene expression of proinflammatory cytokines interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and nitric oxide. Twenty-eight polyphenols, including catechin, phenolic acids, and resveratrol were annotated in peanut skin extract (PSE) with the use of ultra-performance liquid chromatography coupled to quadrupole time of flight mass spectrometry (UPLC-QTOF/MSE) and to the UNIFI Scientific Information System. The administration of PSE at 100 and 150 µg/mL significantly inhibited oxidative stress, by suppressing the production of reactive oxygen species up to 70% and reducing the production of nitric oxide, IL-6 and TNF-α up to 1.7-, 10- and 107-fold, respectively.

Rapid qualitative profiling of metabolites present in Fusarium solani, a rhizospheric fungus derived from Senna spectabilis, using GC/MS and UPLC-QTOF/MSE techniques assisted by UNIFI information system.

Fungi are an important source of natural products found in a variety of plant species. A wide range of methods for the detection of metabolites present in fungi have been reported in the literature. The search for methodologies that allow the rapid detection of compounds present in crude extracts is crucial to enable the metabolite annotation doing a qualitative analysis of the complex matrix. Mass spectrometry is an important ally when it comes to in silico detection of previously reported metabolites. In this work, the ethyl acetate extract of Fusarium solani was analyzed by gas chromatography coupled to mass spectrometry (GC/MS) after derivatization process. The ethyl acetate extract was also investigated by liquid chromatography coupled with high-resolution tandem mass spectrometry assisted by the UNIFI software system. A library containing previously reported metabolites from the Fusarium genus was added to the UNIFI platform. Simultaneously, the extract was analyzed through anticholinesterase and antifungal assays. The analysis of the derivatized extract by GC/MS led to the putative identification of five metabolites, and the investigation using Ultra-High Performance Liquid Chromatography - Quadrupole Time-of-Flight Mass Spectrometry (UPLC-QTOF) analysis in data-independent acquisition mode (mass spectrometry) led to the annotation of 15 compounds present in the built-in Fusarium library added to the UNIFI system. The Fusarium solani extract showed potential anticholinesterase and in vitro antifungal activity supported by the detection of bioactive metabolites.

A utilização da aprendizagem baseada em problema (abp) na formação em saúde: um relato de experiência / The use of problem-based learning (pbl) in health training: a report of experience / El uso del aprendizaje basado en problemas (pbl) en la formación en salud: un informe de experiencia

Introdução:Nos últimos anos tem se evidenciado a necessidade deimplementarnovasmetodologias no ensino, que consigam responder as mudanças tecnológicas, sociais e culturais. Neste cenário, as metodologias ativas vêm se destacando ao colocar o discente no centro do aprendizado, tornando-o parte integral e essencial desse processo. Dentre essas metodologias, está a Aprendizagem Baseada em Problemas. Essa metodologia busca o ensino a partir da solução de problemas, mediante análise em grupo, utilizando-se de conhecimentos previamente adquiridos, facilitandoa apreensãodo conhecimento e valorização do trabalho em equipe.Objetivo:Relatar a experiência de discentes de um curso da área da saúde no desenvolvimento de um caso e sua aplicação no formato de uma Aprendizagem Baseada em Problemas.Método:Estudo descritivo, tipo relato de experiência, onde se elabora o processo de construção desse tipo de aprendizagem e verificação da pertinência da estratégia proposta pordiscentesdeumcurso da saúde da Universidade Federal do Rio Grande do Norte na disciplina de Teoria Geral das Organizações. Resultados:Durante a criação e execução do caso, osdiscentes utilizaramdiversas técnicas para construir ações de enfrentamento ao caso apresentado, como a técnica de Braisntorming, oDiagrama de Causa-Efeito, construção de critérios utilizando materiais do Ministério da Saúde, consultando profissionais do serviço em busca de estratégias viáveis para esses profissionais como forma de validação. Conclusões:O desenvolvimento de um caso utilizando as metodologias que integram a Aprendizagem Baseada em Problemas propiciou aos envolvidos a experimentação de um processo criativo, rico,centrado no conhecimento. A participação dos profissionais das redes de serviços de saúde foi fundamental em todas as fases, evidenciando suapotencialidade formadora indo desde aacademia aosserviços de saúdecom aplicabilidade e eficiência (AU).

Introduction:In recent years, the need to implement new teaching methodologies that can respond to technological, social and cultural changes has been highlighted. In this scenario, active methodologies have been standing out by placing the student at the center of learning, making it an integral and essential part of this process. Among these methodologies is Problem-Based Learning. This methodology seeks to teach from problem solving through group analysis, using previously acquired knowledge, facilitating the understanding of knowledge and the appreciation of teamwork. Objective:Report the experience of health course students in the development of a case and its application in the form of Problem-Based Learning.Methods:This is a descriptive study, type experience report, which describes the process of building Problem-Based Learning and verifying the relevance of the proposed strategy by students of health courses of the Federal University of Rio Grande do Nortein the discipline of General Theory of Organizations.Results:During the creation and execution of the case, the students used various techniques to construct coping actions to the case presented, such as the Braisntorming technique, the Cause-Effect Diagram, criteria construction using Ministry of Health materials, consulting professionals of the service in search for viable strategies for these professionals as a form of validation.Conclusions:The development of a case using the methodologies that integrate Problem-Based Learning has enabled those involved to experiment with a creative, rich, knowledge-centered process. The participation of professionals in the health service networks was essential in all phases, showing their training potential, ranging from the academy to health services with applicability and efficiency (AU).

Introducción:en los últimos años ha sido necesario implementar nuevas metodologías de enseñanza que puedan responder a los cambiostecnológicos, sociales y culturales. En este escenario, las metodologías activas se han destacado al colocar al estudiante en el centro de aprendizaje, convirtiéndolo en una parte integral y esencial de este proceso. Entre estas metodologías se encuentra el Aprendizaje Basado en Problemas. Esta metodología busca la enseñanza desde la resolución de problemas hasta el análisis grupal, utilizando el conocimiento previamente adquirido, facilitando la comprensión del conocimiento y la apreciación del trabajo enequipo.Objetivo:Informar la experiencia de los estudiantes de cursos de salud en el desarrollo de un caso y su aplicación en el formato de Aprendizaje Basado en Problemas. Método:Este es un estudio descriptivo, informe de experiencia tipo, que describeel proceso de construcción de Aprendizaje Basado en Problemas y la verificación de la relevancia de la estrategia propuesta por estudiantes de cursos de salud en la Universidad Federal de Río Grande del Norteen la disciplina de TeoríaGeneral de Organizaciones.Resultados:Durante la creación y ejecución del caso, los estudiantes utilizaron diversas técnicas para construir acciones de afrontamiento al caso presentado, como la técnica de Braisntorming, el Diagrama de Causa-Efecto, la construcción de criterios utilizando materiales del Ministerio de Salud, la consulta de profesionales del servicio en busca de estrategias viables para estos profesionales como forma de validación.Conclusiones:El desarrollo de un caso utilizando las metodologías que integran el aprendizaje basado en problemas ha permitido a los involucrados experimentar con un proceso creativo, rico y centrado en el conocimiento. La participación de profesionales en las redes de servicios de salud fue esencial en todas las fases, mostrando su potencial de capacitación, desde la academia hasta los servicios de salud con aplicabilidad y eficiencia (AU).

Artificial Corn-Based Diet for Rearing Spodoptera frugiperda (Lepidoptera: Noctuidae).

Spodoptera frugiperda (J.E. Smith, 1797) is considered a key pest of maize. However, the artificial diets used for rearing this insect in the laboratory do not contain corn. The aim of this study was to evaluate the biology and to compare the food consumption by S. frugiperda, as well as the food preference of the larvae in the standard diet and the corn-based diet. Three of the following diets were evaluated: a standard diet based on beans (D1), a diet with corn flour as substitute for wheat germ (D2), and a diet replacing beans with green corn (D3). The biological parameters evaluated were period and survival of larvae and pupae; weight of male and female pupae; sex ratio; fecundity; egg incubation period; and adult longevity. The nutritional indices were determined and the biological data obtained were used to determine the parameters of fertility life tables; we also performed a multiple-choice test (feeding test). Larval development of S. frugiperda occurred in all three diets, although without oviposition by females developed from larval fed on D2. There was no difference among the diets in relation to the fertility life table parameters. The diet D2 resulted in better ingestion, digestion, assimilation, and conversion of food, but was associated with a metabolic cost to assimilate the food. Using a multiple-choice test, we observed that the larvae preferred diet D2. Based on our results, the most adequate diets for rearing S. frugiperda in the laboratory are D1 and D3.

Homeologous regulation of Frigida-like genes provides insights on reproductive development and somatic embryogenesis in the allotetraploid Coffea arabica.

Coffea arabica is an allotetraploid of high economic importance. C. arabica transcriptome is a combination of the transcripts of two parental genomes (C. eugenioides and C. canephora) that gave rise to the homeologous genes of the species. Previous studies have reported the transcriptional dynamics of C. arabica. In these reports, the ancestry of homeologous genes was identified and the overall regulation of homeologous differential expression (HDE) was explored. One of these genes is part of the FRIGIDA-like family (FRL), which includes the Arabidopsis thaliana flowering-time regulation protein, FRIGIDA (FRI). As nonfunctional FRI proteins give rise to rapid-cycling summer annual ecotypes instead of vernalization-responsive winter-annuals, allelic variation in FRI can modulate flowering time in A. thaliana. Using bioinformatics, genomic analysis, and the evaluation of gene expression of homeologs, we characterized the FRL gene family in C. arabica. Our findings indicate that C. arabica expresses 10 FRL homeologs, and that, throughout flower and fruit development, these genes are differentially transcribed. Strikingly, in addition to confirming the expression of FRL genes during zygotic embryogenesis, we detected FRL expression during direct somatic embryogenesis, a novel finding regarding the FRL gene family. The HDE profile of FRL genes suggests an intertwined homeologous gene regulation. Furthermore, we observed that FLC gene of C. arabica has an expression profile similar to that of CaFRL genes.

Food Consumption Utilization, and Life History Parameters of Helicoverpa armigera (Lepidoptera: Noctuidae) Reared on Diets of Varying Protein Level.

Helicoverpa armigera (Hübner) is an important pest of crops worldwide, and several studies have focused on the development of this species on different artificial diets. However, studies evaluating the insect's food consumption and utilization using nutritionally different diets are scarce. The aim of this study was to evaluate the biology and to compare the consumption and use of food by H. armigera larvae on diets with different protein levels provided by several dietary ingredients used in the diets. The nutritional index, the relative consumption rate, the relative metabolic rate, the relative growth rate, and the apparent digestibility were higher in the diet with higher than the optimum level of protein. On the other hand, the conversion efficiency of digested food was lower, resulting in a higher metabolic cost. In terms of biological aspects, larval survival was higher for the diet with optimal protein content and lower for the diet with a higher protein level. The pupal period was longer for the diet with a higher protein content, while pupal survival was lower. Among the evaluated diets, the diet with an optimal protein containing white bean 75 g, wheat germ 60 g, soy bran 30 g, milk powder 30 g, brewer's yeast 37.5 g as the protein sources resulted in a higher net reproductive rate, a shorter time for the population to double in number, and the highest rates of population growth. The results suggest that lower or higher protein contents in the diets of H. armigera negatively affect the biological aspects of this species.

Aspectos da assistência de enfermagem para pessoa com insuficiência cardíaca / Aspects of nursing assistance for the person with heart failure / Aspectos de la atención de enfermería para persona com insuficiencia cardíaca

Objetivo: Identificar os aspectos da assistência de enfermagem a pessoas com insuficiência cardíaca crônica, em hospital de especialidade cardiológica. Método: Estudo retrospectivo, de análise documental, em instituição hospitalar cardiológica, com 39 prontuários de pessoas internadas com insuficiência cardíaca, entre 2010 e 2015, sendo coletados dados sociodemográficos e clínico-epidemiológicos e os elementos da assistência de enfermagem, analisados com base na frequência absoluta e relativa. Resultados: Evidenciou-se prevalência dos diagnósticos de enfermagem Risco de infecção e Padrão respiratório ineficaz e intervenções, como Avaliar perfusão periférica, Posicionar em Fowler, Inspecionar pele e Fazer mudança de decúbito. Nos resultados, verificou-se que dez prontuários conseguiram atingir 50% ou mais destes. Conclusão: Foi possível identificar os diagnósticos, as intervenções e os resultados de enfermagem, baseados nos riscos de hospitalização e prevenção secundária cardiovascular, além da prevenção de problemas potenciais, como infecções, lesões e conservação de órgãos-alvo (AU)

Objective: To identify aspects of nursing care for people with chronic heart failure in a cardiology specialty hospital. Method: Retrospective study of documentary analysis, in a cardiac hospital institution, with 39 medical records of hospitalized people with heart failure between 2010 and 2015. Sociodemographic and clinical-epidemiological data were collected, and the elements of nursing care were analyzed based on the absolute and relative frequency. Results: Nursing diagnoses Risk of infection and Ineffective Respiratory Standard, and interventions such as Evaluate peripheral perfusion, Position in Fowler, Inspect skin and Make change of position were prevalent. In the nursing results, only 10 medical records could reach 50% or more of these. Conclusion: We identified nursing diagnoses, interventions and outcomes based on the risks of hospitalization and secondary cardiovascular prevention, as well as the prevention of potential problems such as infections and injuries and conservation of target organs (AU)

Objetivo: identificar aspectos de la atención de enfermería para personas con insuficiencia cardíaca crónica en hospital especializado en cardiología. Método: Estudio retrospectivo de análisis documental, en institución hospitalaria cardíaca, con 39 registros médicos de personas hospitalizadas con insuficiencia cardíaca entre 2010 y 2015, recolectando datos sociodemográficos y clínico-epidemiológicos y los elementos de la atención de enfermería, analizados con base en la frecuencia absoluta y pariente Resultados: Prevalencia de diagnósticos de enfermería Riesgo de Infección y Estandar Respiratorio Ineficaz e intervenciones como Evaluar Perfusión Periférica, Posición en Fowler, Inspeccionar la piel y Cambiar de posición. En los resultados de enfermería se encontró que solo 10 registros médicos podrían alcanzar el 50% o más. Conclusión: fue posible identificar diagnósticos de enfermería, intervenciones y resultados basados en los riesgos de hospitalización y prevención cardiovascular secundaria, así como la prevención de problemas potenciales como infecciones y lesiones y la conservación de los órganos objetivo (AU)

Pesquisa clínica sob a ótica da integridade / Clinical research from the perspective of integrity / La investigación clínica desde la perspectiva de la integridad

Resumo Toda publicação advinda de pesquisa científica exige respeito aos padrões de ética que caracterizam a adequada conduta do investigador, o que se traduz em integridade na pesquisa. Porém, há distinta modalidade de pesquisa, conhecida como "pesquisa clínica", que vem buscando atuar de maneira íntegra visando fortalecer a base de seu agir em prol da sociedade em geral. A caracterização dos envolvidos e sua função ideal e real no cenário moderno foi o objetivo deste trabalho. A busca e descoberta de novas drogas beneficiarão toda coletividade, se as políticas de saúde pública forem eficazes, normatizando o acesso da população a essas descobertas. A integridade na pesquisa, portanto, precisa ampliar seus horizontes para abarcar também a pesquisa clínica, cujos partícipes devem ser orientados e cobrados quanto à boa prática nas diversas etapas de desenvolvimento do estudo, o que permitirá íntegra e exitosa investigação.

Abstract All publications resulting from scientific research require compliance to ethical standards that characterize investigators' appropriate conduct, which translates into integrity in research. However, there is a distinct research modality known as "clinical research", which has been seeking to act with integrity in order to strengthen the basis of its activities carried out for the benefit of society as a whole. The purpose of this work was to identify the people involved in this process as well as their intended and actual role in the modern scenario. The search for and the discovery of new drugs will benefit the entire community, provided that public health policies are efficient - thereby standardizing people's access to those discoveries. Therefore, the concept of integrity in research must expand its horizons in order to also encompass clinical research, in which participants must be guided and charged to apply good practices in the various stages of research development, which will allow for comprehensive and successful research.

Resumen Toda publicación resultante de la investigación científica debe respetar las normas éticas que caracterizan la adecuada conducta del investigador, lo cual se traduce en integridad en la investigación. Sin embargo, hay una modalidad distinta de investigación, conocida como "investigación clínica", que busca actuar de manera íntegra con el fin de fortalecer la base de su accionar para la sociedad en general. El objetivo de este trabajo fue la caracterización de los involucrados y su función ideal y real en el escenario moderno. La búsqueda y el descubrimiento de nuevas drogas beneficiarán a toda la colectividad, si las políticas de salud pública lograran ser eficaces, regulando el acceso de la población a estos descubrimientos. La integridad en la investigación, por lo tanto, necesita ampliar sus horizontes para abarcar también la investigación clínica, cuyos participantes deben estar orientados y advertidos en cuanto a la buena práctica en las diversas etapas de desarrollo del estudio, lo que permitirá una investigación íntegra y exitosa.

Ações de educação em saúde e o planejamento familiar: um relato de experiência / Health education actions and family planning: an experience report

Introdução: Atualmente o planejamento familiar é desenvolvido principalmente pela Atenção Primária nas Unidades Básicas de Saúde a partir das Equipes de Estratégia Saúde da Família, que trás em seu modeloassistêncial o trabalho em equipe, vínculo entre profissionaise comunidade, e participação comunitária.Objetivo: Relatar a experiencia diante da interação ensino-serviço para a formação de futuros profissionais desaúde,apartir da construção de espaços de Educação Popular em Saúde, abordando a temática do planejamento familiar.Metodologia: Trata-se de estudo descritivo,tipo relato de experiência,com construção e execução,a partir de momentos de Educação Popular em Saúde, no município de Barauna, Rio Grande do Norte, entre janeiro e fevereiro de 2018.Resultados: Essa ação resultou na construção de uma peça de Teatro Fórum e de um Quiz realizados nas Unidades de Saúde, com a participação de profissionais e usuáriosdas comunidades,construindo movimentações,com descontração e roda de conversa.Conclusão: A EducaçãoPopular em Saúde mostrou-se como uma ferramenta essencial para o estabelecimento de vínculos entre profissionais e usuários e um potencializador das movimentações deconhecimentos e escutas,construindo espaços coletivos de troca de saberes e experiências (AU).

Introduction: Currently, family planning is developed mainly by Primary Care in Basic Health Units from the Family Health Strategy Teams, which brings teamwork, the link between professionals and the community, as well as community participation, in their model of care.Objective: The experience of teaching-service interaction for the training of future health professionals, from the construction of spaces of Popular Education in Health, addressing the theme of family planning.Methodology: This is a descriptive study, atype of experience report, with construction and execution, starting from moments of Popular Education in Health, in the municipality of Barauna, Rio Grande do Norte, between January and February 2018.Results: This action resulted in the construction of a piece of Theater Forum and a Quiz held in the Health Units, with the participation of professionals and users of the communities, building movements, with relaxation and conversation.Conclusion: Popular Education in Health has proved to be an essential tool for establishing links between professionals and users and a potentiator of knowledge and listening movements, building collective spaces for the exchange of knowledge and experiences (AU).

Cost of Production of Telenomus remus (Hymenoptera: Platygastridae) Grown in Natural and Alternative Hosts.

The study of the technical and economic aspects of rearing natural enemies is essential for its effective use as a biological control agent in the field. The aim of this study was to determine the cost of production of the parasitoid, Telenomus remus (Nixon; Hymenoptera: Platygastridae), reared in eggs of its natural host, Spodoptera frugiperda (J. E. Smith; Lepidoptera: Noctuidae), and the alternative host, Corcyra cephalonica (Stainton; Lepidoptera: Pyralidae). The insects were reared in accordance with their respective methodologies of laboratory rearing. The cost of production of this biocontrol agent is US$ 0.0004 when reared with S. frugiperda eggs and US$ 0.0002 with C. cephalonica eggs.