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1.
Phys Rev Lett ; 125(14): 141103, 2020 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-33064526

RESUMO

Using hydrodynamical simulations for a large set of high-density matter equations of state (EOSs), we systematically determine the threshold mass M_{thres} for prompt black-hole formation in equal-mass and asymmetric neutron star (NS) mergers. We devise the so far most direct, general, and accurate method to determine the unknown maximum mass of nonrotating NSs from merger observations revealing M_{thres}. Considering hybrid EOSs with hadron-quark phase transition, we identify a new, observable signature of quark matter in NS mergers. Furthermore, our findings have direct applications in gravitational wave searches, kilonova interpretations, and multimessenger constraints on NS properties.

2.
Biosens Bioelectron ; 66: 379-84, 2015 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-25463646

RESUMO

We have previously reported the development of an electrochemical method to quantitatively detect vertebrate oestrogens using an oestrogen binding protein (EBP1) present in wild type Saccharomyces cerevisiae and Candida albicans cells. However, the assays were complex and slow with both whole cells and cell lysate. In this work we report the transfer of the EBP1 gene to an industrial yeast, the addition of a his tag sequence to simplify purification of the protein, and the oestrogen binding characteristics of the protein. The recombinant protein (Ebp1p-6h) can now be produced by a non-pathogenic cell, and has shown good stability both when in use and when lyophilised. The detection range covers likely environmental concentrations of free oestrogens and the limit of detection is below the environmental concentration that has significant biological effect. In addition the assay period has been reduced to approximately 2min. This work reports progress toward the construction of a rapid, portable oestrogen sensor that is not restricted to use to the laboratory.


Assuntos
Técnicas Biossensoriais/métodos , Candida albicans/metabolismo , Proteínas de Transporte/metabolismo , Estradiol/análise , Estrogênios/análise , Receptores de Estrogênio/metabolismo , Estradiol/metabolismo , Estrogênios/metabolismo , Humanos , Proteínas Recombinantes/metabolismo
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