Impact of soil density on biomineralization using EICP and MICP techniques for earthen sites consolidation.

Enzyme-induced calcium carbonate precipitation (EICP) and microbially-induced calcium carbonate precipitation (MICP) techniques represent emerging trends in soil stabilization. However, the impact of soil density on biomineralization, particularly in historical earthen sites, remains unclear. This study compares the consolidation effects of EICP and MICP on cylindrical samples (10 cm × 5 cm) with three densities (1.5 g/cm3, 1.6 g/cm3, and 1.7 g/cm3) derived from the soil near the UNESCO World Cultural Heritage Site of Suoyang Ancient City, Gansu Province, China. Results showed that calcium carbonate production increased across all densities through bio-cementation, with higher densities producing more calcium carbonate. MICP-treated specimens exhibited larger increases in calcium carbonate production compared to those treated with EICP. Specimens with a density of 1.7 g/cm³ showed a wave velocity increase of 3.26% (EICP) and 7.13% (MICP), and an unconfined compressive strength increase of 8% (EICP) and 26% (MICP). These strength increases correlated with the generation of calcium carbonate. The findings suggest that biomineralization can be effectively utilized for in situ consolidation of earthen sites, emphasizing the importance of considering soil density in biologically-based conservation technologies. Furthermore, MICP shows potential advantages over EICP in providing stronger, compatible and more sustainable soil reinforcement.

Signatures of Life Detected in Images of Rocks Using Neural Network Analysis Demonstrate New Potential for Searching for Biosignatures on the Surface of Mars.

Microorganisms play a role in the construction or modulation of various types of landforms. They are especially notable for forming microbially induced sedimentary structures (MISS). Such microbial structures have been considered to be among the most likely biosignatures that might be encountered on the martian surface. Twenty-nine algorithms have been tested with images taken during a laboratory experiment for testing their performance in discriminating mat cracks (MISS) from abiotic mud cracks. Among the algorithms, neural network types produced excellent predictions with similar precision of 0.99. Following that step, a convolutional neural network (CNN) approach has been tested to see whether it can conclusively detect MISS in images of rocks and sediment surfaces taken at different natural sites where present and ancient (fossil) microbial mat cracks and abiotic desiccation cracks were observed. The CNN approach showed excellent prediction of biotic and abiotic structures from the images (global precision, sensitivity, and specificity, respectively, 0.99, 0.99, and 0.97). The key areas of interest of the machine matched well with human expertise for distinguishing biotic and abiotic forms (in their geomorphological meaning). The images indicated clear differences between the abiotic and biotic situations expressed at three embedded scales: texture (size, shape, and arrangement of the grains constituting the surface of one form), form (outer shape of one form), and pattern of form arrangement (arrangement of the forms over a few square meters). The most discriminative components for biogenicity were the border of the mat cracks with their tortuous enlarged and blistered morphology more or less curved upward, sometimes with thin laminations. To apply this innovative biogeomorphological approach to the images obtained by rovers on Mars, the main physical and biological sources of variation in abiotic and biotic outcomes must now be further considered.

Intertidal biodiversity and physical habitat complexity on historic masonry walls: A comparison with modern concrete infrastructure and natural rocky cliffs.

Maritime built heritage (e.g., historic seawalls) represents an important component of coastal infrastructure around the world. Despite this, the ecological communities supported by these structures are poorly understood. At seven locations across the UK, we compared the biodiversity and physical habitat characteristics of (1) historic (pre-1900s) masonry walls, (2) concrete walls, and (3) natural rocky cliffs. Historic masonry walls were found to support significantly more species than concrete walls, and in some locations, more diverse communities than nearby rocky cliffs. Nevertheless, community composition remained distinct between the three habitat types at each location. We also found that historic masonry walls provided substantially more cryptic space (i.e., crevices) than both concrete walls and rocky cliffs, and this is positively associated with the ecological value of these structures. Overall, our results suggest that the unique physical properties of historic masonry walls make them an important component of habitat diversity along developed coastlines.

Determining Water Transport Kinetics in Limestone by Dual-Wavelength Cavity Ring-Down Spectroscopy.

Water plays a major role in the deterioration of porous building materials such as those widely found in built heritage, influencing many physical, chemical, and biological decay processes. This article details a proof-of-principle study using near-infrared cavity ring-down spectroscopy (CRDS) to monitor the release of water and its artificially enriched isotopologues from small (ca. 25 × 25 × 5 mm) samples of limestone subject to drying by a fixed flow of nitrogen with varying levels of humidity and at room temperature and atmospheric pressure. Under low-humidity conditions, the drying kinetics are consistent with the well-established two-phase drying process exhibited by porous materials, namely, an initial constant drying rate period (phase I) followed by a falling drying rate period (phase II). The water diffusivity during phase II, DII, was measured (for Clipsham limestone) to be 3.0 × 10-9 ± 1 × 10-10 m2 s-1. The CRDS measurements allow spectroscopic determination of the total mass of water released by the sample, and the calculated values are in excellent agreement with gravimetric analysis. Importantly, the selectivity and sensitivity afforded by CRDS allows isotope analysis to be carried out, such that the flux of isotopically labeled water out of the sample can be determined under conditions of humidified flow where there may be a simultaneous ingress of water from the environment. Dual-wavelength CRDS distinguishes isotopic species, and it is demonstrated that the drying kinetics and physical properties of the samples are self-consistent when monitoring both HDO and H2O (for HDO, DII was 3.2 × 10-9 ± 4 × 10-10 m2 s-1). As the humidity levels in the flow increase, a departure from the distinct two-phase behavior is observed in the HDO drying curves. These new measurements of isotopically resolved mass fluxes will help refine models for drying mechanisms in porous media.

Moisture content and material density affects severity of frost damage in earthen heritage.

Successful conservation of earthen heritage requires an understanding of interactions between environmental and climatic conditions, soil-based materials and human interventions. Frost cycling is likely to be an important contributor to the deterioration of earthen heritage, with frost damage known to cause deterioration features such as flaking and granular disintegration. However, it is not clear how important a contribution frost cycling makes in comparison with other agents. Previous earthen heritage studies have focused on other agents of deterioration, such as wind and rain, or investigated the role of freeze-thaw cycles under conditions unrepresentative of many earthen sites. We investigate how density and moisture content affect the severity of frost damage on earthen heritage using materials and conditions informed by those found at earthen sites in NW China. We prepared rammed earth cubes (5 × 5 × 5 cm) at two densities (1.65 and 1.75 g cm-3) and with five moisture content levels between 0.46 and 8%. Samples were subjected to 80 freeze-thaw temperature cycles (+7 to -15 °C) in an environmental cabinet. Deterioration was recorded using visual assessment, measurements of surface roughness, ultrasonic pulse velocities, a Vicat needle test and mass loss. Results showed frost damage was dependent on moisture content and density of rammed earth samples. Samples with <2% moisture content showed no visible frost damage. Samples with higher moisture contents (>6%) and higher densities exhibited the greatest deterioration with surface granulation, salt efflorescence and flakes detaching from the parent cubes. This suggests that frost damage to rammed earth is likely to be focused in periods when rainfall or snowmelt is followed by freeze-thaw temperature cycling. In addition, results suggest that if higher density earthen materials are used for repairs or restoration for sites in dryland environments, these could be more vulnerable to frost damage than lower density materials.

Stone-built heritage as a proxy archive for long-term historical air quality: A study of weathering crusts on three generations of stone sculptures on Broad Street, Oxford.

Black crusts on historic buildings are mainly known for their aesthetic and deteriorative impacts, yet they also can advance air pollution research. Past air pollutants accumulate in distinct layers of weathering crusts. Recent studies have used these crusts to reconstruct pollution to improve our understanding of its effects on stone-built heritage. However, the majority of the studies provide only coarse resolution reconstruction of pollution, able to distinguish between 'inner = old' and 'outer = modern' crust layers. In contrast, very few studies have linked distinct periods of exposure to pollution variations in the composition of these crusts. Here we address this research gap by developing a finer-scale resolution pollution record. Our study explored the unique configuration of limestone sculptures in central Oxford, which have been exposed over the last 350 years to three different periods of atmospheric pollution; the early Industrial Revolution, the Victorian period and the 20th century. When the first two generations of sculptures were moved to less polluted areas, their 'pollution clocks' were stopped. Here we discuss the potential of investigating the 'pollution clock' recorded in the geochemical makeup of each sculpture generation's weathering crust layers. We found the analysed crusts record clear changes related to the evolution of modes of transport and industrial and technological development in Oxford. Higher levels of Arsenic (As), Selenium (Se) are linked to pollution from coal burning during Victorian times and Lead (Pb) indicated leaded petrol use in modern times. Our work shows that stone-built heritage with a known history of air pollution exposure allows improving the pollution reconstruction resolution of these weathering crusts. The results provide the basis for calibrating long-term geochemical archives. This approach may be used to reconstruct past air quality and has the potential to inform stone weathering research and conservation, in addition to improving the reconstruction of historical pollution.

Deterioration risk of dryland earthen heritage sites facing future climatic uncertainty.

Uncertainties over future climatic conditions pose significant challenges when selecting appropriate conservation strategies for heritage sites. Choosing effective strategies is especially important for earthen heritage sites located in dryland regions, as many are experiencing rapid environmentally-driven deterioration. We use a newly developed cellular automaton model (ViSTA-HD), to evaluate the environmental deterioration risk, over a 100-year period, under a range of potential climate and conservation scenarios. Results show increased wind velocities could substantially increase the overall deterioration risk, implying the need for wind-reducing conservation strategies. In contrast, predicted increases in rainfall are not likely to increase the overall deterioration risk, despite greater risk of rain-driven deterioration features. Of the four conservation strategies tested in our model, deterioration risk under all climatic scenarios was best reduced by increasing the coverage of natural, randomly-distributed vegetation to 80%. We suggest this approach could be an appropriate long-term conservation strategy for other earthen sites in dryland regions.

Impact of colour on the bioreceptivity of granite to the green alga Apatococcus lobatus: Laboratory and field testing.

Recent work has demonstrated that surface colour affects the formation of cyanobacterial subaerial biofilms on polycarbonate coupons and, in turn, influences their bioreceptivity. To explore whether colour is important on other substrates, the influence of colour on the primary bioreceptivity of granite to the terrestrial green alga Apatococcus lobatus (Trebouxiophyceae, Chlorophyta) has been assessed. Two granitoids (Grissal and Rosa Porriño) with the same texture, and very similar chemical composition, open porosity and surface roughness, but different coloration related to feldspars (i.e. greyish and reddish) were used to conduct bioreceptivity studies in parallel field and laboratory tests. Light microscopy, colour spectrophotometry, PAM fluorometry, and optical profilometry were used to evaluate colonisation and its impacts. Short-term results (after 7 and 10 weeks of colonisation by a mono-species biofilm) from both lab and field trials, showed significantly more algae growth on reddish granite (Rosa Porriño) than on greyish granite (Grissal). Also, optical profilometry and light microscopy demonstrated that on both granites algal aggregates developed in hollows. We attribute the roughly double levels of A. lobatus growth on reddish vs greyish granite to differences in the amount of radiant energy absorbed and the higher levels of red wavelength light (known to encourage algal growth) reflected from the reddish surface.

A review of the nature, role and control of lithobionts on stone cultural heritage: weighing-up and managing biodeterioration and bioprotection.

Lithobionts (rock-dwelling organisms) have been recognized as agents of aesthetic and physico-chemical deterioration of stonework. In consequence, their removal from cultural heritage stone surfaces (CHSS) is widely considered a necessary step in conservation interventions. On the other hand, lithobiontic communities, including microbial biofilms ('biological patinas'), can help integrate CHSS with their environmental setting and enhance biodiversity. Moreover, in some cases bioprotective effects have been reported and even interpreted as potential biotechnological solutions for conservation. This paper reviews the plethora of traditional and innovative methodologies to characterize lithobionts on CHSS in terms of biodiversity, interaction with the stone substrate and impacts on durability. In order to develop the best management and conservation strategies for CHSS, such diagnosis should be acquired on a case-by-case basis, as generalized approaches are unlikely to be suitable for all lithobionts, lithologies, environmental and cultural contexts or types of stonework. Strategies to control biodeteriogenic lithobionts on CHSS should similarly be based on experimental evaluation of their efficacy, including long-term monitoring of the effects on bioreceptivity, and of their environmental safety. This review examines what is known about the efficacy of control methods based on traditional-commercial biocides, as well as those based on innovative application of substances of plant and microbial origin, and physical techniques. A framework for providing a balanced scientific assessment of the role of lithobionts on CHSS and integrating this knowledge into management and conservation decision-making is presented.

The Search for a Signature of Life on Mars: A Biogeomorphological Approach.

Geological evidence shows that life on Earth evolved in line with major concomitant changes in Earth surface processes and landforms. Biogeomorphological characteristics, especially those involving microorganisms, are potentially important facets of biosignatures on Mars and are generating increasing interest in astrobiology. Using Earth as an analog provides reasons to suspect that past or present life on Mars could have resulted in recognizable biogenic landforms. Here, we discuss the potential for, and limitations of, a biogeomorphological approach to identifying the subsets of landforms that are modulated or created through biological processes and thus present signatures of life on Mars. Subsets especially involving microorganisms that are potentially important facets of biosignatures on Mars are proposed: (i) weathering features, biocrusts, patinas, and varnishes; (ii) microbialites and microbially induced sedimentary structures (MISS); (iii) bioaccumulations of skeletal remains; (iv) degassing landforms; (v) cryoconites; (vi) self-organized patterns; (vii) unclassified non-analog landforms. We propose a biogeomorphological frequency histogram approach to identify anomalies/modulations in landform properties. Such detection of anomalies/modulations will help track a biotic origin and lead to the development of an integrative multiproxy and multiscale approach combining morphological, structural, textural, and geochemical expertise. This perspective can help guide the choice of investigation sites for future missions and the types and scales of observations to be made by orbiters and rovers.

Wind-driven rain and future risk to built heritage in the United Kingdom: Novel metrics for characterising rain spells.

Wind-driven rain (WDR) is rain given a horizontal velocity component by wind and falling obliquely. It is a prominent environmental risk to built heritage, as it contributes to the damage of porous building materials and building element failure. While predicted climate trends are well-established, how they will specifically manifest in future WDR is uncertain. This paper combines UKCP09 Weather Generator predictions with a probabilistic process to create hourly time series of climate parameters under a high-emissions scenario for 2070-2099 at eight UK sites. Exposure to WDR at these sites for baseline and future periods is calculated from semi-empirical models based on long-term hourly meteorological data using ISO 15927-3:2009. Towards the end of the twenty-first century, it is predicted that rain spells will have higher volumes, i.e. a higher quantity of water will impact façades, across all 8 sites. Although the average number of spells is predicted to remain constant, they will be shorter with longer of periods of time between them and more intense with wind-driven rain occurring for a greater proportion of hours within them. It is likely that in this scenario building element failure - such as moisture ingress through cracks and gutter over-spill - will occur more frequently. There will be higher rates of moisture cycling and enhanced deep-seated wetting. These predicted changes require new metrics for wind-driven rain to be developed, so that future impacts can be managed effectively and efficiently.

Thermal blanketing by ivy (Hedera helix L.) can protect building stone from damaging frosts.

The impact of plants growing on buildings remains controversial, especially for vulnerable historic walls and ruins requiring on-going conservation. English ivy (Hedera helix L.) can cause considerable damage where it is able to grow into deteriorating masonry, yet in some circumstances it may be protective. Here we focus on the potential of ivy to buffer damaging thermal cycles and frost events that can contribute to the deterioration of masonry materials. On limestone masonry test walls in central Southern England (Wytham near Oxford, UK), ivy foliage had a significant influence on stone-surface freezing regimes. Over two successive winters (2012/13 and 2013/14) the frequency of freezing events under ivy was reduced on average by 26%, their duration by 34% and their severity by 32%. A subsequent laboratory simulation showed that stone mass loss, surface softening, and textural development were all significantly reduced under an 'ivy covered' thermal regime. Cautious extrapolation indicates that ivy can reduce frost-driven granular-scale decay of limestone by the order of 30 g m-2 yr-1, depending on the local freezing regime. Whilst the capacity of ivy to cause damage should not be underplayed, vertical greenery can aid heritage conservation efforts by mitigating specific environmental threats.

Comparing the effectiveness of hyperspectral imaging and Raman spectroscopy: a case study on Armenian manuscripts.

There is great practical and scholarly interest in the identification of pigments in works of art. This paper compares the effectiveness of the widely used Raman Spectroscopy (RS), with hyperspectral imaging (HSI), a reflectance imaging technique, to evaluate the reliability of HSI for the identification of pigments in historic works of art and to ascertain if there are any benefits from using HSI or a combination of both. We undertook a case study based on six Armenian illuminated manuscripts (eleventh-eighteenth centuries CE) in the Bodleian Library, University of Oxford. RS, and HSI (380-1000 nm) were both used to analyse the same 10 folios, with the data then used to test the accuracy and efficiency of HSI against the known results from RS using reflectance spectra reference databases compiled by us for the project. HSI over the wavelength range 380-1000 nm agreed with RS at best 93% of the time, and performance was enhanced using the SFF algorithm and by using a database with many similarities to the articles under analysis. HSI is significantly quicker at scanning large areas, and can be used alongside RS to identify and map large areas of pigment more efficiently than RS alone. HSI therefore has potential for improving the speed of pigment identification across manuscript folios and artwork but must be used in conjunction with a technique such as RS.

Durability of anti-graffiti coatings on stone: natural vs accelerated weathering.

Extending the use of novel anti-graffiti coatings to built heritage could be of particular interest providing the treatments are efficient enough in facilitating graffiti removal and long-lasting to maintain their protective properties without interfering with the durability of the substrates. However, studies of the durability of these coatings are scarce and have been mainly carried out under accelerated weathering conditions, the most common practice for assessing the durability of materials but one that does not reproduce accurately natural working conditions. The present study aimed to assess the durability of the anti-graffiti protection afforded by two anti-graffiti treatments (a water dispersion of polyurethane with a perfluoropolyether backbone and a water based crystalline micro wax) on Portland limestone and Woodkirk sandstone after 1 year of outdoor exposure in the South of England with periodic painting and cleaning episodes taking place. A parallel study under artificial weathering conditions in a QUV chamber for 2000 hours was also carried out. Changes to the coatings were assessed by measuring colour, gloss, water-repellency, roughness and microstructure, the latter through micro-Raman and optical microscope observations, periodically during the experiments. The results show that both anti-graffiti treatments deteriorated under both artificial and natural weathering conditions. For the polyurethane based anti-graffiti treatment, artificial ageing produced more deterioration than 1 year of outdoor exposure in the south of England due to loss of adhesion from the stones, whereas for micro wax coating there were no substantial differences between the two types of weathering.

Cool barnacles: Do common biogenic structures enhance or retard rates of deterioration of intertidal rocks and concrete?

Sedentary and mobile organisms grow profusely on hard substrates within the coastal zone and contribute to the deterioration of coastal engineering structures and the geomorphic evolution of rocky shores by both enhancing and retarding weathering and erosion. There is a lack of quantitative evidence for the direction and magnitude of these effects. This study assesses the influence of globally-abundant intertidal organisms, barnacles, by measuring the response of limestone, granite and marine-grade concrete colonised with varying percentage covers of Chthamalus spp. under simulated, temperate intertidal conditions. Temperature regimes at 5 and 10mm below the surface of each material demonstrated a consistent and statistically significant negative relationship between barnacle abundance and indicators of thermal breakdown. With a 95% cover of barnacles, subsurface peak temperatures were reduced by 1.59°C for limestone, 5.54°C for concrete and 5.97°C for granite in comparison to no barnacle cover. The amplitudes of short-term (15-30min) thermal fluctuations conducive to breakdown via 'fatigue' effects were also buffered by 0.70°C in limestone, 1.50°C in concrete and 1.63°C in granite. Furthermore, concentrations of potentially damaging salt ions were consistently lower under barnacles in limestone and concrete. These results indicate that barnacles do not enhance, but likely reduce rates of mechanical breakdown on rock and concrete by buffering near-surface thermal cycling and reducing salt ion ingress. In these ways, we highlight the potential role of barnacles as agents of bioprotection. These findings support growing international efforts to enhance the ecological value of hard coastal structures by facilitating their colonisation (where appropriate) through design interventions.

Predicting the long-term durability of hemp-lime renders in inland and coastal areas using Mediterranean, Tropical and Semi-arid climatic simulations.

Hemp-based composites are eco-friendly building materials as they improve energy efficiency in buildings and entail low waste production and pollutant emissions during their manufacturing process. Nevertheless, the organic nature of hemp enhances the bio-receptivity of the material, with likely negative consequences for its long-term performance in the building. The main purpose of this study was to study the response at macro- and micro-scale of hemp-lime renders subjected to weathering simulations in an environmental cabinet (one year was condensed in twelve days), so as to predict their long-term durability in coastal and inland areas with Mediterranean, Tropical and Semi-arid climates, also in relation with the lime type used. The simulated climatic conditions caused almost unnoticeable mass, volume and colour changes in hemp-lime renders. No efflorescence or physical breakdown was detected in samples subjected to NaCl, because the salt mainly precipitates on the surface of samples and is washed away by the rain. Although there was no visible microbial colonisation, alkaliphilic fungi (mainly Penicillium and Aspergillus) and bacteria (mainly Bacillus and Micrococcus) were isolated in all samples. Microbial growth and diversification were higher under Tropical climate, due to heavier rainfall. The influence of the bacterial activity on the hardening of samples has also been discussed here and related with the formation and stabilisation of vaterite in hemp-lime mixes. This study has demonstrated that hemp-lime renders show good durability towards a wide range of environmental conditions and factors. However, it might be useful to take some specific preventive and maintenance measures to reduce the bio-receptivity of this material, thus ensuring a longer durability on site.

The influence of the type of lime on the hygric behaviour and bio-receptivity of hemp lime composites used for rendering applications in sustainable new construction and repair works.

The benefits of using sustainable building materials are linked not only to the adoption of manufacturing processes that entail reduced pollution, CO2 emissions and energy consumption, but also to the onset of improved performance in the building. In particular, hemp-lime composite shows low shrinkage and high thermal and acoustic insulating properties. However, this material also shows a great ability to absorb water, an aspect that can turn out to be negative for the long-term durability of the building. For this reason, the hygric properties of hemp-based composites need to be studied to ensure the correct use of this material in construction and repair works. The water absorption, drying and transpirability of hemp composites made with aerial (in the form of dry powder and putty) and hydraulic limes were investigated here and related to the microbial growth induced by the water movements within the material. Results show that hemp-natural hydraulic lime mixes exhibit the highest transpirability and drying rate, the lowest water absorption by immersion and capillary uptake and the least intense microbial attack and chromatic change. A microscopical study of the hemp shives also related their great ability to absorb water to the near-irreversible swelling of their structure under dry-wet conditions.

The spatial organization and microbial community structure of an epilithic biofilm.

Microbial biofilms are common on lithic surfaces, including stone buildings. However, the ecology of these communities is poorly understood. Few studies have focused on the spatial characteristics of lithobiontic biofilms, despite the fact that spatial structure has been demonstrated to influence ecosystem function (and hence biodegradation) and community diversity. Furthermore, relatively few studies have utilized molecular techniques to characterize these communities, even though molecular methods have revealed unexpected microbial diversity in other habitats. This study investigated (1) the spatial structure and (2) the taxonomic composition of an epilithic biofilm using molecular techniques, namely amplicon pyrosequencing and terminal restriction fragment length polymorphism. Dispersion indices and Mantel correlograms were used to test for the presence of spatial structure in the biofilm. Diversity metrics and rank-abundance distributions (RADs) were also generated. The study revealed spatial structure on a centimetre scale in eukaryotic microbes (fungi and algae), but not the bacteria. Fungal and bacterial communities were highly diverse; algal communities much less so. The RADs were characterized by a distinctive 'hollow' (concave up) profile and long tails of rare taxa. These findings have implications for understanding the ecology of epilithic biofilms and the spatial heterogeneity of stone biodeterioration.

Algal 'greening' and the conservation of stone heritage structures.

In humid, temperate climates, green algae can make a significant contribution to the deterioration of building stone, both through unsightly staining ('greening') and, possibly, physical and chemical transformations. However, very little is known about the factors that influence the deteriorative impact and spatial distribution of green algal biofilms, hindering attempts to model the influence of climate change on building conservation. To address this problem, we surveyed four sandstone heritage structures in Belfast, UK. Our research had two aims: 1) to investigate the relationships between greening and the deterioration of stone structures and 2) to assess the impacts of environmental factors on the distribution of green biofilms. We applied an array of analytical techniques to measure stone properties indicative of deterioration status (hardness, colour and permeability) and environmental conditions related to algal growth (surface and sub-surface moisture, temperature and surface texture). Our results indicated that stone hardness was highly variable but only weakly related to levels of greening. Stone that had been exposed for many years was, on average, darker and greener than new stone of the same type, but there was no correlation between greening and darkening. Stone permeability was higher on 'old', weathered stone but not consistently related to the incidence of greening. However, there was evidence to suggest that thick algal biofilms were capable of reducing the ingress of moisture. Greening was negatively correlated with point measurements of surface temperature, but not moisture or surface texture. Our findings suggested that greening had little impact on the physical integrity of stone; indeed the influence of algae on moisture regimes in stone may have a broadly bioprotective action. Furthermore, the relationship between moisture levels and greening is not straightforward and is likely to be heavily dependent upon temporal patterns in moisture regimes and other, unmeasured, factors such as nutrient supply.