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J Clin Microbiol ; 36(9): 2408-12, 1998 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-9705364

RESUMO

Enteroviruses (EV) are among the most common causes of aseptic meningitis. Standard diagnostic techniques are often too slow and lack sensitivity to be of clinical relevance. EV RNA can be detected within 5 h by a commercially available reverse transcription-PCR (RT-PCR) test kit. Cerebrospinal fluid (CSF) samples from 68 patients presenting with aseptic meningitis during a summer outbreak in Switzerland were examined in parallel with cell culture and commercial RT-PCR. RT-PCR was positive in all 16 CSF specimens positive by cell culture (100%). In addition, 42 of 52 (80%) CSF samples negative by cell culture were PCR positive. In 26 of these 42 (62%) patients, viral culture from other sites (throat swab or stool) was also positive. The CSF virus culture took 3 to 7 days to become positive. Echovirus 30 was the type most often isolated in this outbreak. The sensitivity of CSF RT-PCR based on clinical diagnosis during this aseptic meningitis outbreak in patients with negative bacterial culture results was 85%, i.e., considerably higher than the sensitivity of CSF virus culture (24%). We conclude that this commercial RT-PCR assay allows a positive diagnosis with minimal delay and may thus influence clinical decisions.


Assuntos
Surtos de Doenças , Infecções por Enterovirus/líquido cefalorraquidiano , Infecções por Enterovirus/virologia , Enterovirus/isolamento & purificação , Meningite Viral/líquido cefalorraquidiano , Meningite Viral/virologia , Adolescente , Adulto , Criança , Pré-Escolar , Infecções por Enterovirus/epidemiologia , Feminino , Humanos , Lactente , Masculino , Meningite Viral/epidemiologia , Reação em Cadeia da Polimerase/métodos , Suíça
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