Histopathological Biocompatibility Evaluation of TheraCal PT, NeoMTA, and MTA Angelus in a Murine Model.

The aim of this study was to evaluate the biocompatibility of the regeneration of the dentin-pulp complex in a murine model with different treatments with MTA Angelus, NeoMTA, and TheraCal PT. An in vivo controlled experimental study of 15 male Wistar rats forming three study groups, the upper and lower central incisors were selected where pulpotomies were conducted, leaving a central incisor as control at 15, 30, and 45 days. For data analysis, these were expressed as mean ± standard deviation and were examined by Kruskal-Wallis test. Three factors were analyzed as follows: "inflammatory infiltrate; disorganization of pulp tissue, and the formation of reparative dentin". No statistical significance was found between the different groups (p > 0.05). Treatment with these three biomaterials (MTA, TheraCal PT, and Neo MTA) presented an inflammatory infiltrate and slight disorganization of the odontoblast layer in the pulp tissue of a murine model, with normal coronary pulp tissue and the formation of reparative dentin in the three experimental groups. Thus, we are able to conclude that all three are biocompatible materials.

Rhabdomyosarcoma of the head and neck in pediatric patients: a systematic review

Background: Rhabdomyosarcoma (RMS) is a soft tissue malignant tumor of mesenchymal cell origin, which usually shows variable differentiation of muscle cells. It is the most common solid sarcoma in children. The most usual site of occurrence are the head and neck regions. RMS presents a variety of histologic features, and so differential diagnosis with other small round cell tumors is needed. Hence, it has been very useful to the field to undertake additional immunohistochemical studies to determine the diagnosis and, on occasions, to assign subtype tumors. Material and methods: A systematic review of three databases (Medline, Biological Science Collection and Health & Medical Collection) was carried out with the purpose of analyzing rhabdomyosarcoma cases reported in the literature, specifically with localization in the head and neck regions in children. This strategy allowed us to identify the main anatomical site of appearance, the subtype of RMS, average age, histologic characteristics and immunohistochemistry markers used in a usual and any additional way. Results: According to the selection criteria in this systematic review, twelve articles, and fourteen cases were identified that highlight that the histological diagnosis usually presents cellular heterogeneity. Therefore, immunohistochemistry is needed to confirm the diagnosis. Conclusions: Histologic characterization is not always sufficient for a conclusive diagnosis of RMS. Therefore, immunohistochemistry is helpful to determine the subtype and consequently, sometimes the behavior, treatment and prognosis. Additional markers may vary according to the institution and the need of particular cases. (AU)

Antifungal biomaterial for reducing infections caused by Candida albicans in edentulous patients.

INTRODUCTION: Polymethylmethacrylate (PMMA) acrylic resins are used to make dentures for edentulous patients. OBJECTIVE: To find out the prevalence of Candida species in patients with and without removable prostheses from a dental clinic in León, Guanajuato, as well as to assess the antifungal effect and biological behavior of an experimental PMMA with silver nanoparticles for its possible application in prostheses. METHOD: To identify Candida species, smear samples were obtained from the palatal mucosa of 140 patients aged ≥ 60 years. The experimental PMMA with silver nnoparticles was placed in Candida albicans cultures, which were stained with the Live/Dead® kit for analysis under confocal microscopy; subsequently, it was implanted in Wistar rats in order to know its behavior in the surrounding tissues. RESULTS: Candida albicans was the most prevalent species in the evaluated patients, followed by Candida tropicalis and Candida krusei. The acrylic resin with silver nanoparticles significantly decreased the presence of Candida albicans. In the animal model, a discrete and controlled inflammatory reaction was found, which indicated biocompatibility of the acrylic resin that was used. CONCLUSIONS: It is possible for the nanostructured material with antifungal effect to be used in order to promote the reduction of oral Candida infections in edentulous patients.

INTRODUCCIÓN: Las resinas acrílicas de polimetilmetacrilato (PMMA) son utilizadas para elaborar dentaduras para pacientes edéntulos. OBJETIVO: Conocer la prevalencia de las especies de Candida en pacientes con y sin prótesis removibles de una clínica de odontología en León, Guanajuato; así como valorar el efecto antifúngico y el comportamiento biológico de un PMMA experimental con nanopartículas de plata para su posible aplicación en prótesis. MÉTODO: Para identificar las especies de Candida se obtuvieron muestras para frotis de la mucosa palatina de 140 pacientes con edad ≥ 60 años. El PMMA experimental con nanopartículas de plata fue colocado en cultivos de Candida albicans, los cuales fueron teñidos con el kit Live/Dead® para su análisis bajo microscopia confocal; posteriormente, se implantó en ratas Wistar para conocer su comportamiento en los tejidos circundantes. RESULTADOS: Candida albicans fue la especie más prevalente en los pacientes valorados, seguida de Candida tropicalis y Candida krusei. La resina acrílica con nanopartículas de plata disminuyó significativamente la presencia de Candida albicans. En el modelo animal se encontró reacción inflamatoria discreta y controlada, lo cual indicó la biocompatibilidad de la resina acrílica utilizada. CONCLUSIONES: Es posible utilizar el material nanoestructurado con efecto antifúngico para promover la reducción de infecciones orales por Candida en pacientes edéntulos.

Cáncer en pacientes Jóvenes (Parte 1)Análisis clínico e histopatológico de carcinoma de células escamosas de la cavidad bucal en pacientes jóvenes. Un estudio descriptivo y comparativo en México / Cancer in young patients (Part 1). Clinical and histopathological analysis of squamous cell carcinoma of the oral cavity in young patients A descriptive and comparative study in Mexico

El cáncer en cavidad bucal y de la región de cabeza y cuello se presenta con mayor frecuencia entre la quinta y sexta década de la vida generalmente atribuido al uso indiscriminado de substancias como el alcohol y el tabaco por un periodo de tiempo considerable. Sin embargo estudios recientes demuestran un incremento en la incidencia en pacientes jóvenes que nunca estuvieron expuestos a estos y a otros factores como riesgo ocupacional, predisposición genética, dieta...

Oral cavity and head and neck cancer occurs most often between the fifth and sixth decade of life and is generally attributed to the indiscriminate use of substances such as alcohol and tobacco snuff for a considerable amount of time. However, recent studies show an increased incidence in younger patients who have never been exposed to these and other risk factors such as occupational risk, genetic predisposition and diet...