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Appl Spectrosc ; 67(9): 1013-8, 2013 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24067631

RESUMO

We measured the photothermal lens signal in samples exhibiting high turbidity using a pump-probe scheme. We show that the photothermal lens signal properties remain nearly unchanged up to values of turbidity of 6 cm(-1) despite the signal reduction due to the decrease of excitation power associated to turbidity losses. The signal starts decreasing abruptly for values of turbidity larger than 6 cm(-1). Multiple light scattering yields a reduction of the temperature gradients, which results in a decrease of the effective signal. However, the signal-to-noise ratio remains above 50 for turbidity values of 9 cm(-1), which corresponds to a reduction of light transmission by more than four orders of magnitude. We report on the detection of the photothermal lens signal through a 2 mm layer of organic tissue with a signal-to-noise ratio of about 500. This technique appears promising for imaging applications in organic samples, which usually exhibit high turbidity for visible and near-infrared light.


Assuntos
Nefelometria e Turbidimetria/métodos , Análise Espectral/métodos , Animais , Galinhas , Lentes , Luz , Músculo Esquelético/química , Nefelometria e Turbidimetria/instrumentação , Espalhamento de Radiação , Razão Sinal-Ruído , Análise Espectral/instrumentação , Termodinâmica
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