RESUMO
Engineered adipose tissues are developed for their use as substitutes for tissue replacement in reconstructive surgery. To ensure a timely perfusion of the grafted substitutes, different strategies can be used such as the incorporation of an endothelial component. In this study, we engineered human adipose tissue substitutes comprising of functional adipocytes as well as a natural extracellular matrix using the self-assembly approach, without the use of exogenous scaffolding elements. Human microvascular endothelial cells (hMVECs) were incorporated during tissue production in vitro and we hypothesized that their presence would favor the early connection with the host vascular network translating into functional enhancement after implantation into nude mice in comparison to the substitutes that were not enriched in hMVECs. In vitro, no significant differences were observed between the substitutes in terms of histological aspects. After implantation, both groups presented numerous adipocytes and an abundant matrix in addition to the presence of host capillaries within the grafts. The substitutes thickness and volume were not significantly different between groups over the short-term time course of 14 days (d). For the microvascularized adipose tissues, human CD31 staining revealed a human capillary network connecting with the host microvasculature as early as 3 d after grafting. The detection of murine red blood cells within human CD31+ structures confirmed the functionality of the human capillary network. By analyzing the extent of the global vascularization achieved, a tendency towards increased total capillary network surface and volume was revealed for prevascularized tissues over 14 d. Therefore, applying this strategy on thicker reconstructed adipose tissues with rate-limiting oxygen diffusion might procure added benefits and prove useful to provide voluminous substitutes for patients suffering from adipose tissue loss or defects.
Assuntos
Tecido Adiposo/metabolismo , Prótese Vascular , Células Endoteliais/citologia , Engenharia Tecidual/métodos , Adipócitos/citologia , Adulto , Animais , Capilares/metabolismo , Meios de Cultivo Condicionados , Eritrócitos/metabolismo , Matriz Extracelular/metabolismo , Feminino , Humanos , Técnicas In Vitro , Masculino , Camundongos , Camundongos Nus , Microcirculação , Neovascularização Fisiológica , Molécula-1 de Adesão Celular Endotelial a Plaquetas/química , Células Estromais/citologiaRESUMO
Salmonella enterica serovar Heidelberg (S. Heidelberg) is one of the top serovars causing human salmonellosis. This serovar ranks second and third among serovars that cause human infections in Québec and Canada, respectively, and has been associated with severe infections. Traditional typing methods such as PFGE do not display adequate discrimination required to resolve outbreak investigations due to the low level of genetic diversity of isolates belonging to this serovar. This study evaluates the ability of four whole genome sequence (WGS)-based typing methods to differentiate among 145â¯S. Heidelberg strains involved in four distinct outbreak events and sporadic cases of salmonellosis that occurred in Québec between 2007 and 2016. Isolates from all outbreaks were indistinguishable by PFGE. The core genome single nucleotide variant (SNV), core genome multilocus sequence typing (MLST) and whole genome MLST approaches were highly discriminatory and separated outbreak strains into four distinct phylogenetic clusters that were concordant with the epidemiological data. The clustered regularly interspaced short palindromic repeats (CRISPR) typing method was less discriminatory. However, CRISPR typing may be used as a secondary method to differentiate isolates of S. Heidelberg that are genetically similar but epidemiologically unrelated to outbreak events. WGS-based typing methods provide a highly discriminatory alternative to PFGE for the laboratory investigation of foodborne outbreaks.
Assuntos
Tipagem de Sequências Multilocus/métodos , Intoxicação Alimentar por Salmonella/microbiologia , Infecções por Salmonella/microbiologia , Salmonella enterica/isolamento & purificação , Sequenciamento Completo do Genoma/métodos , Técnicas de Tipagem Bacteriana/métodos , Genoma Bacteriano , Humanos , Filogenia , Quebeque/epidemiologia , Intoxicação Alimentar por Salmonella/epidemiologia , Infecções por Salmonella/epidemiologia , Salmonella enterica/classificação , Salmonella enterica/genéticaRESUMO
Fecal microbiota transplantation (FMT) may be a novel approach to eliminate multidrug-resistant bacteria from the gut and to prevent future infections. Using whole metagenome sequencing data from 8 FMT donor-recipient pairs, we identified 37 and 95 antimicrobial resistance genes that were acquired by or removed from FMT recipients, respectively.
Assuntos
Infecções por Clostridium/terapia , Farmacorresistência Bacteriana Múltipla/genética , Transplante de Microbiota Fecal/efeitos adversos , Microbioma Gastrointestinal , Genes MDR , Antibacterianos/farmacologia , Fezes/microbiologia , Genes Bacterianos , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , RecidivaRESUMO
Ultrasound (US) guided needle positioning is safer than anatomical landmark techniques for central venous access. Hand-eye coordination and execution time depend on the professional's ability, previous training and personal skills. Needle guidance positioning systems (GPS) may theoretically reduce execution time and facilitate needle positioning in specific targets, thus improving patient comfort and safety. Three groups of healthcare professionals (41 anaesthesiologists and intensivists, 41 residents in anaesthesiology and intensive care, 39 nurse anaesthetists) were included and required to perform 3 tasks (positioning the tip of a needle in three different targets in a silicon phantom) by using successively a conventional US-guided needle positioning and a needle GPS. We measured execution times to perform the tasks, hand-eye coordination and the number of repositioning occurrences or errors in handling the needle or the probe. Without the GPS system, we observed a significant inter-individual difference for execution time (P<0.05), hand-eye coordination and the number of errors/needle repositioning between physicians, residents and nurse anaesthetists. US training and video gaming were found to be independent factors associated with a shorter execution time. Use of GPS attenuated the inter-individual and group variability. We observed a reduced execution time and improved hand-eye coordination in all groups as compared to US without GPS. Neither US training, video gaming nor demographic personal or professional factors were found to be significantly associated with reduced execution time when GPS was used. US associated with GPS systems may improve safety and decrease execution time by reducing inter-individual variability between professionals for needle-handling procedures.
Assuntos
Anestesiologistas , Agulhas , Enfermeiros Anestesistas , Adulto , Competência Clínica , Cuidados Críticos , Feminino , Humanos , Internato e Residência , Masculino , Pessoa de Meia-Idade , Conforto do Paciente , Segurança do Paciente , Imagens de Fantasmas , Médicos , Estudos Prospectivos , Desempenho Psicomotor , Jogos de VídeoRESUMO
BACKGROUND: Clostridium difficile infection (CDI) is the leading infectious cause of nosocomial diarrhea. Hospitalized patients are at increased risk of developing CDI because they are exposed to C. difficile spores through contact with the hospital environment and often receive antibiotics and other medications that can disrupt the integrity of the indigenous intestinal microbiota and impair colonization resistance. Using whole metagenome shotgun sequencing, we examined the diversity and composition of the fecal microbiota in a prospective cohort study of 98 hospitalized patients. RESULTS: Four patients had asymptomatic C. difficile colonization, and four patients developed CDI. We observed dramatic shifts in the structure of the gut microbiota during hospitalization. In contrast to CDI cases, asymptomatic patients exhibited elevated relative abundance of potentially protective bacterial taxa in their gut at the onset of C. difficile colonization. Use of laxatives was associated with significant reductions in the relative abundance of Clostridium and Eubacterium; species within these genera have previously been shown to enhance resistance to CDI via the production of secondary bile acids. Cephalosporin and fluoroquinolone exposure decreased the frequency of Clostridiales Family XI Incertae Sedis, a bacterial family that has been previously associated with decreased CDI risk. CONCLUSIONS: This study underscores the detrimental impact of antibiotics as well as other medications, particularly laxatives, on the intestinal microbiota and suggests that co-colonization with key bacterial taxa may prevent C. difficile overgrowth or the transition from asymptomatic C. difficile colonization to CDI.
Assuntos
Antibacterianos/efeitos adversos , Clostridioides difficile/patogenicidade , Infecção Hospitalar/microbiologia , Diarreia/microbiologia , Enterocolite Pseudomembranosa/microbiologia , Microbioma Gastrointestinal/genética , Metagenoma , Idoso , Ácidos e Sais Biliares/biossíntese , Cefalosporinas/efeitos adversos , Clostridioides difficile/efeitos dos fármacos , Clostridioides difficile/crescimento & desenvolvimento , Infecção Hospitalar/patologia , Diarreia/etiologia , Diarreia/patologia , Enterocolite Pseudomembranosa/etiologia , Enterocolite Pseudomembranosa/patologia , Eubacterium/efeitos dos fármacos , Eubacterium/crescimento & desenvolvimento , Eubacterium/patogenicidade , Feminino , Fluoroquinolonas/efeitos adversos , Humanos , Laxantes/efeitos adversos , Masculino , Pessoa de Meia-Idade , Estudos ProspectivosRESUMO
Clostridium difficile infection (CDI) is intricately linked to the health of the gastrointestinal tract and its indigenous microbiota. In this study, we assessed whether fecal excretion of host DNA is associated with CDI development. Assuming that shedding of epithelial cell increases in the inflamed intestine, we used human DNA excretion as a marker of intestinal insult. Whole-genome shotgun sequencing was employed to quantify host DNA excretion and evaluate bacterial content in fecal samples collected from patients with incipient CDI, hospitalized controls, and healthy subjects. Human DNA excretion was significantly increased in patients admitted to the hospital for a gastrointestinal ailment, as well as prior to an episode of CDI. In multivariable analyses, human read abundance was independently associated with CDI development. Host DNA proportions were negatively correlated with intestinal microbiota diversity. Enterococcus and Escherichia were enriched in patients excreting high quantities of human DNA, while Ruminococcus and Odoribacter were depleted. These findings suggest that intestinal inflammation can occur prior to CDI development and may influence patient susceptibility to CDI. The quantification of human DNA in feces could serve as a simple and noninvasive approach to assess bowel inflammation and identify patients at risk of CDI.
Assuntos
Infecções por Clostridium/genética , Infecções por Clostridium/microbiologia , DNA/genética , Fezes/química , Fezes/microbiologia , Estudos de Casos e Controles , Clostridioides difficile/patogenicidade , Células Epiteliais/microbiologia , Feminino , Humanos , Intestinos/microbiologia , Masculino , Microbiota/fisiologiaRESUMO
The development of tissue-engineered substitutes of substantial volume is closely associated with the need to ensure rapid vascularization upon grafting. Strategies promoting angiogenesis include the in vitro formation of capillary-like networks within engineered substitutes. We generated both connective and adipose tissues based on a cell sheet technology using human adipose-derived stromal cells. This study evaluates the morphology and extent of the capillary networks that developed upon seeding of human microvascular endothelial cells during tissue production. We posited that adipocyte presence/secretory products could modulate the resulting capillary network when compared to connective substitutes. Analyses including confocal imaging of CD31-labeled capillary-like networks indicated slight differences in their morphological appearance. However, the total volume occupied by the networks as well as the frequency distribution of the structure's volumes were similar between connective and adipose tissues. The average diameter of the capillary structures tended to be 20% higher in reconstructed adipose tissues. Quantification of pro-angiogenic molecules in conditioned media showed greater amounts of leptin (15×), angiopoietin-1 (3.4×) and HGF (1.7×) secreted from adipose than connective tissues at the time of endothelial cell seeding. However, this difference was attenuated during the following coculture period in endothelial cell-containing media, correlating with the minor differences noted between the networks. Taken together, we developed a protocol allowing reconstruction of both connective and adipose tissues featuring well-developed capillary networks in vitro. We performed a detailed characterization of the network architecture within engineered tissues that is relevant for graft assessment before implantation as well as for in vitro screening of angiogenic modulators using three-dimensional models.
Assuntos
Tecido Adiposo/irrigação sanguínea , Capilares/citologia , Capilares/crescimento & desenvolvimento , Tecido Conjuntivo/irrigação sanguínea , Células Endoteliais/fisiologia , Neovascularização Fisiológica/fisiologia , Engenharia Tecidual/métodos , Adipócitos , Tecido Adiposo/citologia , Tecido Adiposo/fisiologia , Proteínas Angiogênicas/metabolismo , Células Cultivadas , Técnicas de Cocultura , Tecido Conjuntivo/anatomia & histologia , Tecido Conjuntivo/fisiologia , Células Endoteliais/citologia , Humanos , Via SecretóriaRESUMO
Clostridium difficile infection (CDI) is the most important cause of nosocomial diarrhea. Broad-spectrum antimicrobials have profound detrimental effects on the structure and diversity of the indigenous intestinal microbiota. These alterations often impair colonization resistance, allowing the establishment and proliferation of C. difficile in the gut. Studies involving animal models have begun to decipher the precise mechanisms by which the intestinal microbiota mediates colonization resistance against C. difficile and numerous investigations have described gut microbiota alterations associated with C. difficile colonization or infection in human subjects. Fecal microbiota transplantation (FMT) is a highly effective approach for the treatment of recurrent CDI that allows the restoration of a healthy intestinal ecosystem via infusion of fecal material from a healthy donor. The recovery of the intestinal microbiota after FMT has been examined in a few reports and work is being done to develop custom bacterial community preparations that could be used as a replacement for fecal material.
RESUMO
BACKGROUND: Antimicrobial use is thought to suppress the intestinal microbiota, thereby impairing colonization resistance and allowing Clostridium difficile to infect the gut. Additional risk factors such as proton-pump inhibitors may also alter the intestinal microbiota and predispose patients to Clostridium difficile infection (CDI). This comparative metagenomic study investigates the relationship between epidemiologic exposures, intestinal bacterial populations and subsequent development of CDI in hospitalized patients. We performed a nested case-control study including 25 CDI cases and 25 matched controls. Fecal specimens collected prior to disease onset were evaluated by 16S rRNA gene amplification and pyrosequencing to determine the composition of the intestinal microbiota during the at-risk period. RESULTS: The diversity of the intestinal microbiota was significantly reduced prior to an episode of CDI. Sequences corresponding to the phylum Bacteroidetes and to the families Bacteroidaceae and Clostridiales Incertae Sedis XI were depleted in CDI patients compared to controls, whereas sequences corresponding to the family Enterococcaceae were enriched. In multivariable analyses, cephalosporin and fluoroquinolone use, as well as a decrease in the abundance of Clostridiales Incertae Sedis XI were significantly and independently associated with CDI development. CONCLUSIONS: This study shows that a reduction in the abundance of a specific bacterial family - Clostridiales Incertae Sedis XI - is associated with risk of nosocomial CDI and may represent a target for novel strategies to prevent this life-threatening infection.
RESUMO
Closely related strains of Escherichia coli have been shown to cause extraintestinal infections in unrelated persons. This study tests whether a food reservoir may exist for these E. coli. Isolates from 3 sources over the same time period (2005-2007) and geographic area were compared. The sources comprised prospectively collected E. coli isolates from women with urinary tract infection (UTI) (n = 353); retail meat (n = 417); and restaurant/ready-to-eat foods (n = 74). E. coli were evaluated for antimicrobial drug susceptibility and O:H serotype and compared by using 4 different genotyping methods. We identified 17 clonal groups that contained E. coli isolates (n = 72) from >1 source. E. coli from retail chicken (O25:H4-ST131 and O114:H4-ST117) and honeydew melon (O2:H7-ST95) were indistinguishable from or closely related to E. coli from human UTIs. This study provides strong support for the role of food reservoirs or foodborne transmission in the dissemination of E. coli causing common community-acquired UTIs.
Assuntos
Reservatórios de Doenças , Infecções por Escherichia coli/etiologia , Microbiologia de Alimentos , Infecções Urinárias/etiologia , Escherichia coli Uropatogênica , Adolescente , Adulto , Animais , Galinhas/microbiologia , Cucurbitaceae/microbiologia , Impressões Digitais de DNA , Escherichia coli/classificação , Infecções por Escherichia coli/classificação , Infecções por Escherichia coli/epidemiologia , Feminino , Genótipo , Humanos , Carne/microbiologia , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Ontário/epidemiologia , Quebeque/epidemiologia , Restaurantes , Sorotipagem , Infecções Urinárias/microbiologia , Escherichia coli Uropatogênica/genética , Escherichia coli Uropatogênica/isolamento & purificação , Adulto JovemRESUMO
Discriminatory genotyping methods for the analysis of Escherichia coli other than O157:H7 are necessary for public health-related activities. A new multi-locus variable number tandem repeat analysis protocol is presented; this method achieves an index of discrimination of 99.5% and is reproducible and valid when tested on a collection of 836 diverse E. coli.
Assuntos
Técnicas de Tipagem Bacteriana/métodos , Impressões Digitais de DNA/métodos , DNA Bacteriano/genética , Infecções por Escherichia coli/microbiologia , Escherichia coli/classificação , Carne/microbiologia , Repetições Minissatélites , Animais , Análise por Conglomerados , Escherichia coli/genética , Escherichia coli/isolamento & purificação , Genótipo , Humanos , Epidemiologia Molecular/métodos , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
Differences and similarities between Escherichia coli O157:H7 isolates from beef cattle and those from sporadic human outbreaks are not fully elucidated. Here, we compared 44 O157:H7 isolates of bovine and human origins (22 isolates of each) to better understand their cytotoxic potential. The Shiga toxin genes stx1, stx2, or both were detected in the 44 isolates, and all elicited Vero cell cytotoxicity. The greatest cytotoxicity was caused by bovine isolates having only stx2 and which represented the majority of such isolates (81.8%). However, no correlation was found between the level of stx gene transcription and cytotoxicity. All human and bovine isolates possessed variant type stx2 and stx2c, respectively, as determined by PCR-restriction fragment length polymorphism. Isolates harboring both stx1 and stx2 genes were much more frequent in human isolates (86.4%). The combination stx1-stx2c found in only four bovine isolates was less cytotoxic. It is clear that cytotoxicity alone cannot account for the apparent inability of O157:H7 bovine isolates to cause diseases in humans. We have found that stx1-stx2-containing or stx1-stx2c-containing isolates were less cytotoxic than several bovine isolates having only stx2c, suggesting that the stx gene combination or other virulence genes in specific genetic lineages may affect the disease outcome.
Assuntos
Bovinos/microbiologia , Morte Celular/efeitos dos fármacos , Escherichia coli O157/isolamento & purificação , Escherichia coli O157/patogenicidade , Toxina Shiga I/toxicidade , Toxina Shiga II/toxicidade , Animais , Chlorocebus aethiops , Colite/microbiologia , DNA Bacteriano/análise , Infecções por Escherichia coli/microbiologia , Escherichia coli O157/genética , Fezes/microbiologia , Expressão Gênica , Humanos , Carne/microbiologia , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , Toxina Shiga I/genética , Toxina Shiga II/genética , Células Vero/efeitos dos fármacosRESUMO
Women with urinary tract infections (UTIs) in California, USA (1999-2001), were infected with closely related or indistinguishable strains of Escherichia coli (clonal groups), which suggests point source dissemination. We compared strains of UTI-causing E. coli in California with strains causing such infections in Montréal, Québec, Canada. Urine specimens from women with community-acquired UTIs in Montréal (2006) were cultured for E. coli. Isolates that caused 256 consecutive episodes of UTI were characterized by antimicrobial drug susceptibility profile, enterobacterial repetitive intergenic consensus 2 PCR, serotyping, XbaI and NotI pulsed-field gel electrophoresis, multilocus sequence typing, and phylogenetic typing. We confirmed the presence of drug-resistant, genetically related, and temporally clustered E. coli clonal groups that caused community-acquired UTIs in unrelated women in 2 locations and 2 different times. Two clonal groups were identified in both locations. Epidemic transmission followed by endemic transmission of UTI-causing clonal groups may explain these clusters of UTI cases.
Assuntos
Surtos de Doenças , Infecções por Escherichia coli/epidemiologia , Infecções por Escherichia coli/microbiologia , Escherichia coli/classificação , Infecções Urinárias/epidemiologia , Infecções Urinárias/microbiologia , Adolescente , Adulto , Técnicas de Tipagem Bacteriana/métodos , California/epidemiologia , Análise por Conglomerados , Infecções Comunitárias Adquiridas/epidemiologia , Infecções Comunitárias Adquiridas/microbiologia , Estudos Transversais , Impressões Digitais de DNA , Farmacorresistência Bacteriana Múltipla , Escherichia coli/efeitos dos fármacos , Escherichia coli/genética , Escherichia coli/isolamento & purificação , Feminino , Humanos , Pessoa de Meia-Idade , Filogenia , Quebeque/epidemiologia , SorotipagemRESUMO
The ability to harvest and culture stem cell populations from various human postnatal tissues is central to regenerative medicine applications, including tissue engineering. The discovery of multipotent mesenchymal stem cells within the stromal fraction of adipose tissue prompted their use for the healing and reconstruction of many tissues. Here, we examined the influence of adipose-derived stem/stromal cells (ASCs) on skin's regenerative processes, from a tissue engineering perspective. Using a self-assembly approach, human skin substitutes were produced. They featured a stromal compartment containing human extracellular matrix endogenously produced from either dermal fibroblasts or adipose-derived stem/stromal cells differentiated or not toward the adipogenic lineage. Human keratinocytes were seeded on each stroma and cultured at the air-liquid interface to reconstruct a bilayered skin substitute. These new skin substitutes, containing an epidermis and a distinctive stroma devoid of synthetic biomaterial, displayed characteristics similar to human skin. The influence of the type of stromal compartment on epidermal morphogenesis was assessed by the evaluation of tissue histology, the expression of key protein markers of the epidermal differentiation program (keratin [K] 14, K10, transglutaminase), the expression of dermo-epidermal junction components (laminins, collagen VII), and the presence of basement membrane and hemidesmosomes. Our findings suggest that adipose-derived stem/stromal cells could usefully substitute dermal fibroblasts for skin reconstruction using the self-assembly method. Finally, by exploiting the adipogenic potential of ASCs, we also produced a more complete trilayered skin substitute consisting of the epidermis, the dermis, and the adipocyte-containing hypodermis, the skin's deepest layer. Disclosure of potential conflicts of interest is found at the end of this article.
Assuntos
Tecido Adiposo/citologia , Pele Artificial , Células-Tronco/citologia , Células Estromais/citologia , Engenharia Tecidual , Animais , Membrana Basal/citologia , Compartimento Celular , Diferenciação Celular , Separação Celular , Células Cultivadas , Derme/citologia , Células Epidérmicas , Epiderme/transplante , Fibroblastos/citologia , Humanos , Camundongos , Camundongos Nus , Células Estromais/transplante , Tela Subcutânea/metabolismoRESUMO
SV40 large T antigen (T-ag) is a multifunctional protein that successively binds to 5'-GAGGC-3' sequences in the viral origin of replication, melts the origin, unwinds DNA ahead of the replication fork, and interacts with host DNA replication factors to promote replication of the simian virus 40 genome. The transition of T-ag from a sequence-specific binding protein to a nonspecific helicase involves its assembly into a double hexamer whose formation is likely dictated by the propensity of T-ag to oligomerize and its relative affinities for the origin as well as for nonspecific double- and single-stranded DNA. In this study, we used a sensitive assay based on fluorescence anisotropy to measure the affinities of wild-type and mutant forms of the T-ag origin-binding domain (OBD), and of a larger fragment containing the N-terminal domain (N260), for different DNA substrates. We report that the N-terminal domain does not contribute to binding affinity but reduces the propensity of the OBD to self-associate. We found that the OBD binds with different affinities to its four sites in the origin and determined a consensus binding site by systematic mutagenesis of the 5'-GAGGC-3' sequence and of the residue downstream of it, which also contributes to affinity. Interestingly, the OBD also binds to single-stranded DNA with an approximately 10-fold higher affinity than to nonspecific duplex DNA and in a mutually exclusive manner. Finally, we provide evidence that the sequence specificity of full-length T-ag is lower than that of the OBD. These results provide a quantitative basis onto which to anchor our understanding of the interaction of T-ag with the origin and its assembly into a double hexamer.