Assuntos
Carbapenêmicos/farmacologia , Infecções por Pseudomonas/microbiologia , Pseudomonas aeruginosa/efeitos dos fármacos , Pseudomonas aeruginosa/enzimologia , Resistência beta-Lactâmica , beta-Lactamases/análise , Antibacterianos/farmacologia , Brasil , Hospitais , Humanos , Pacientes Internados , Pseudomonas aeruginosa/isolamento & purificação , beta-Lactamases/isolamento & purificaçãoRESUMO
Andean potato mottle virus (APMV), an endemic South American comovirus, has a bipartite genome consisting of two plus-strand RNA molecules (M and B RNA). We have cloned the 3' half of the B RNA and identified the complete sequence of the putative APMV RNA polymerase. The RNA polymerase gene is part of a large polyprotein-encoding open reading frame. The putative, mature RNA polymerase, as deduced by comparison with the related cowpea mosaic virus (CPMV), type member of the comovirus group, is 703 amino acids long and shows a large degree of similarity with CPMV and other RNA polymerases.
Assuntos
RNA Polimerases Dirigidas por DNA/genética , Genes Virais/genética , Vírus de Plantas/genética , RNA Viral/genética , Solanum tuberosum/microbiologia , Sequência de Aminoácidos , Sequência de Bases , Clonagem Molecular , Dados de Sequência Molecular , Vírus de Plantas/enzimologia , Homologia de SequênciaRESUMO
The transference of the genetic markers and the presence of DNA plasmidial in 240 cultures of Escherichia coli was investigated. The strains were originated from Waste Treatment Plant (inffluent and effluents) located in Ilha do Governador, Rio de Janeiro. By conjugation analysis, E. coli K 12 allowed the isolation of the transconjugants resistant to antibiotics Su, Sm, Tc, Cm, Ap; heavy metals (Cu, Hg and Zn) and colicinogenic factors (Ia, Ib and V) mainly in coliforms isolated Cm and Ap from the terminals of the treatment plant. The percentual distribution of the plasmids was prevalent in the cultures of E. coli originated from material collected in the effluents and reached a rate higher than 65%.