RESUMO
The changes in the neuroactive amino acid contents, GABA metabolism and TCA reactions have been studied in rat brain regions under experimental morphine withdrawal (MW). MW was developed by means of the cessation of morphine intraperitoneal injections 1 and 36 hours, 3 and 7 days after the course of morphine administration for 7 days. In cortex the significant increase in the contents of glutamate, glutamine, asparagine, and alanine was observed in remote terms of MW. In cerebellum MW led to the decrease in the levels of glutamine and asparagine and increase in glycine level, followed by the GABA-transaminase activation and the succinate dehydrogenase inhibition. In thalamus prolongation of MW caused to the further inhibition of the activities of the GABA-catabolising enzymes. The changes observed in the amino acids levels and the GABA shunt activity are likely to be explained by indirect adaptation of the brain regions differing in the opioid receptors contents to protracted morphine administration.
Assuntos
Aminoácidos/metabolismo , Encéfalo/metabolismo , Morfina/efeitos adversos , Entorpecentes/efeitos adversos , Síndrome de Abstinência a Substâncias/metabolismo , Tálamo/metabolismo , Ácido gama-Aminobutírico/metabolismo , 4-Aminobutirato Transaminase/metabolismo , Animais , Cerebelo/efeitos dos fármacos , Cerebelo/metabolismo , Ativação Enzimática , Masculino , Ratos , Tálamo/efeitos dos fármacosRESUMO
The influence of chronic morphine intoxication (for 7, 14 and 21-days) has been studied on the contents of neuroactive amino acids (GABA, glutamate, glutamine, alanine, aspartate, glycine, taurine) and on the activities of their metabolizing enzymes (GABA-transaminase, succinic semialdehyde dehydrogenase, succinic dehydrogenase, alanine- and aspartate-aminotransferase) in the rat brain regions: cortex, brain stem and cerebellum. We detected the significant decrease of glutamate level and the enhancement of GABA and aspartate levels in cortex after long-term morphine administration. In brain stem the increase in the contents of GABA, glutamine and taurine was noted together with the tendency in attenuation of this effect when intoxication was prolonged. The dose-dependent enhancement in the levels of glutamate, aspartate and glycine was observed after longer courses of morphine administration. In cerebellum the 21-day morphine administration led to attenuation of the morphine-induced increase in the contents of GABA, glutamate, alanine and glycine.
Assuntos
Aminoácidos/metabolismo , Encéfalo/efeitos dos fármacos , Morfina/intoxicação , Entorpecentes/intoxicação , Neurotransmissores/metabolismo , 4-Aminobutirato Transaminase/metabolismo , Alanina Transaminase/metabolismo , Aldeído Oxirredutases/metabolismo , Animais , Aspartato Aminotransferases/metabolismo , Encéfalo/metabolismo , Tronco Encefálico/efeitos dos fármacos , Tronco Encefálico/metabolismo , Cerebelo/efeitos dos fármacos , Cerebelo/metabolismo , Córtex Cerebral/efeitos dos fármacos , Córtex Cerebral/metabolismo , Masculino , Ratos , Succinato Desidrogenase/metabolismo , Succinato-Semialdeído Desidrogenase , Taurina/metabolismo , Ácido gama-Aminobutírico/metabolismoAssuntos
Consumo de Bebidas Alcoólicas/tratamento farmacológico , Fígado/efeitos dos fármacos , Extratos Vegetais/uso terapêutico , Síndrome de Abstinência a Substâncias/tratamento farmacológico , Consumo de Bebidas Alcoólicas/metabolismo , Animais , Metabolismo dos Lipídeos , Fígado/enzimologia , Fígado/metabolismo , Ratos , Síndrome de Abstinência a Substâncias/metabolismoRESUMO
The metabolic disturbances caused by compulsory drug intake into animals and men were characterized. The probable mechanisms of these disturbances are discussed.
