Emergence of coherent wave groups in deep-water random sea.

Extreme surface waves in a deep-water long-crested sea are often interpreted as a manifestation in the real world of the so-called breathing solitons of the focusing nonlinear Schrödinger equation. While the spontaneous emergence of such coherent structures from nonlinear wave dynamics was demonstrated to take place in fiber-optics systems, the same point remains far more controversial in the hydrodynamic case. With the aim to shed further light on this matter, the emergence of breatherlike coherent wave groups in a long-crested random sea is investigated here by means of high-resolution spectral simulations of the fully nonlinear two-dimensional Euler equations. The primary focus of our study is to parametrize the structure of random wave fields with respect to the Benjamin-Feir index, which is a nondimensional measure of the energy localization in Fourier space. This choice is motivated by previous results, showing that extreme-wave activity in a long-crested sea is highly sensitive to such a parameter, which is varied here by changing both the characteristic spectral bandwidth and the average wave steepness. It is found that coherent wave groups, closely matching realizations of Kuznetsov-Ma breathers in Euler dynamics, develop within wave fields characterized by sufficiently narrow-banded spectra. The characteristic spatial and temporal scales of wave group dynamics, and the corresponding occurrence of extreme events, are quantified and discussed by means of space-time autocorrelations of the surface elevation envelope and extreme-event statistics.

An improved procedure for low-temperature embedding of high-pressure frozen and freeze-substituted plant tissues resulting in excellent structural preservation and contrast.

Here we describe refinements in the processing of high-pressure frozen samples of delicate plant tissues for immuno-electron microscopy. These involve: shortened freeze-substitution schedules, lower temperatures during processing and polymerisation, the avoidance of temperature fluctuations and the optimisation of heat transfer from the specimens using small disposable aluminium containers. The application of these modifications leads to very good structural preservation and selective membrane contrast. As a result, the versatility of the method is increased since not only immuno-electron microscopical studies can be performed but often the quality is also quite suitable for structural investigations.

Antagonistic activity of HIV-1 T helper peptides flanked by an unrelated carrier protein.

Antagonism is the ability of a modified antigenic peptide (altered peptide ligand, APL) to prevent CD4 T cell activation by the original peptide. Here we show that antagonistic activity can be conferred to peptides of HIV envelope glycoprotein gp120 and reverse transcriptase p66 by adding flanking polypeptide sequences at the C or at the N terminus by genetic engineering, rather than by introducing substitutions by synthesis. The glutathione S-transferase (GST)-peptide system has been used to produce molecules that display the peptide at the appropriate end of the GST carrier. When the gp120 peptide 191-205 (pep24) was expressed at the C terminus of GST (GST-24), antigenicity of specific human CD4 T cells was maintained. In contrast, when the peptide was expressed at the N terminus of GST (24-GST), antigenicity was abolished and antagonistic activity was introduced. Similar results were obtained with a p66-derived peptide at the C terminus of the GST carrier. Antagonism was (1) specific; proliferation of a CD4 T cell line from the same donor responding to the envelope glycoprotein of another retrovirus, HTLV-1, was not affected; (2) reversible; proliferative response was rescued in T cells exposed to antigen-presenting cells (APC) pulsed with the antagonist; (3) dominant; T cells cultured with APC pulsed with the agonist and with APC pulsed with the antagonist did not proliferate. The carrier could be cleaved by proteolysis while the antagonistic activity was preserved. Thus a minimal sequence that confers antagonistic activity can be engineered or synthesized with peptides to antagonize undesired CD4 responses as an alternative to the use of APL.