[Study on the kinetics of isolation of trypsin immobilized on dialdehyde cellulose during hydrolytic destruction]. / Issledovanie kinetiki vydeleniia tripsina, immobilizovannogo na dial'degidtselliuloze, pri gidroliticheskoi destruktsii.

The kinetics of isolation of trypsin immobilized on a dialdehyde cellulose carrier in buffer solutions with pH 7.0 and 8.0 was studied. The number of the aldehyde groups amounted to 15-60 per cent of the initial one. The kinetics of hydrolytic destruction of the collected samples with the immobilized trypsin and the carrier in buffer solutions with pH 7.0 and 8.0 was also studied. The constants of the rates of trypsin isolation from the materials and the constants of the rates of liberation into the buffer solutions of the compounds containing CO-groups were determined. The groups were used for estimating hydrolytic destruction of the materials and the time of complete destruction of the materials in the solutions.

[Transformation of free radicals of gamma-irradiated enzymes during long-term storage]. / Prevrashchenie svobodnykh radikalov v gamma-obluchennykh fermentakh pri dlitel'nom khranenii.

The ESR method was used to study free radical processes occurring in gamma-irradiated enzymes upon their long-term storage (up to 4 years) both at room temperature and at 4 degrees C. Simultaneously, studied was the biological activity of gamma-irradiated enzymes subjected to a long-term storage. The immobilization of enzymes on dialdehyde cellulose or polycaproamine was shown to preserve their biological activity during the postirradiation storage.