Expression and degranulation of mast cells in laser photobiomodulated mucositis chemo-induced: pilot study in hamsters.

This study aimed to evaluate the influence of laser photobiomodulation on the expression and degranulation of mast cells in chemo-induced oral mucositis (OM) lesions in hamsters. Twelve adult male Syrian hamsters (Mesocricetus auratus), golden lineage, were submitted to OM induction. They were divided into three groups: control-OM without treatment (C), OM treated with red laser (RL), OM treated with infrared laser (IL) and analyzed in the experimental time of 7 days. Three and 4 days after the intraperitoneal injection of the chemotherapy drug fluorouracil, the OM lesions were induced by making grooves in the right cheek pouch. Immediately after chemoinduction, the hamsters were submitted to photobiomodulation every 48 h for 7 days. The specimens were processed and stained using the hematoxylin-eosin and toluidine blue techniques. There was a predominance of mild chronic inflammation in the experimental groups and a greater persistence of neutrophils in the control group (C), although not statistically significant. The group irradiated with red laser (RL) had the highest mean mast cell expression (38.28 ± 19.05) (p < 0.001). As for the degranulation activity in mast cells, the control group (C) showed a greater number of fields with more than 50% of degranulated cells, presenting statistical significance when comparing it with the RL (p < 0.009) and IL (p = 0.036) group. It can be concluded that photobiomodulation, at both wavelengths, decreased mast cell degranulation, accelerating the inflammatory process. The use of infrared laser provided, in addition to less degranulation, the quantitative reduction of mast cells.

Mass change of a sodium bicarbonate air-polished nanocomposite exposed to cigarette smoke, coffee, and red wine / Perda de massa de nanocompósito jateado com bicarbonato de sódio e exposto à fumaça de cigarro, café e vinho tinto

Introdução: As resinas compostas estão sujeitas à desafios na cavidade oral relacionados aos hábitos dos pacientes, que podem comprometer a integridade e longevidade das restaurações. As consequências da associação do consumo de bebidas e o uso de cigarros à profilaxia profissional precisam ser estudadas. Objetivo: O objetivo desse estudo foi avaliar como o jateamento com bicarbonato de sódio afeta a mudança de massa (sorção e solubilidade) de uma resina composta exposta à fumaça de cigarro, vinho tinto e café. Material e método: Oitenta corpos-de-prova de resina composta (FiltekZ350 XT - 3M/ESPE) foram preparados e distribuídos em 8 grupos (n=10): G1 (jateamento + água destilada), G2 (jateamento + café), G3 (jateamento + vinho tinto), G4 (jateamento + fumaça de cigarro), G5 (água destilada), G6 (café), G7 (vinho tinto) e G8 (fumaça de cigarro). Os corpos-de-prova de resina composta foram pesados em três tempos diferentes para obter M1 (massa inicial), M2 (massa após 30 dias de armazenamento nas soluções testadas e exposição ao agente) e M3 (após dessecação). Os valores de sorção e solubilidade foram calculados e analisados usando ANOVA 2-fatores e teste Tukey (5%). Resultado: Os grupos experimentais tiveram maior sorção e solubilidade comparado ao grupo controle, independentemente do uso do jateamento. Não houve diferença estatística na sorção para o fator jateamento. Porém, a solubilidade foi maior nos grupos jateados. Conclusão: O jateamento com bicarbonato de sódio foi capaz de intensificar a solubilidade da resina testada, embora não tenha aumentado significativamente os valores de sorção.

Introduction: Composite resins are subject to challenges in the oral cavity that are related to patients' habits, which can compromise the restorations' integrity and longevity. Therefore, it is necessary to study how consuming beverages and smoking cigarettes affects professional prophylaxis. Objective: The aim of this study was to evaluate how the sodium bicarbonate air-polishing affects the mass change (sorption and solubility) of a composite resin exposed to cigarette smoke, red wine, or coffee solution. Material and method: Eighty composite resin samples (FiltekZ350 XT - 3M/ESPE) were prepared and distributed into 8 groups (n=10): G1 (air-polishing + distilled water), G2 (air-polishing + coffee), G3 (air-polishing + red wine), G4 (air-polishing + cigarette smoke), G5 (distilled water), G6 (coffee), G7 (red wine), or G8 (cigarette smoke). The composite resin samples were weighed in triplicate to obtain M1 (initial mass), M2 (mass after 30 days of storage in the tested solutions), and M3 (after desiccation) values. The sorption and solubility values were calculated and analyzed using 2-way ANOVA and Tukey's test (5%). Result: The experimental groups had higher sorption and solubility values than the control groups, regardless of whether air-polishing was used. There was no statistically significant difference in the sorption between the air-polished and non-air-polished groups; however, the solubility was higher in the air-polished groups. Conclusion: Air-polishing using sodium bicarbonate powder was able to intensify the solubility process of the tested resins but did not significantly increase the sorption values.

Effect of air-polishing on properties of nanocomposite submitted to coffee, red wine and cigarette smoke

AIM: The aim of this study was to evaluate the surface roughness and the color stability of nanocomposite exposed to the sodium bicarbonate air-polishing (SBAP) followed by red wine, coffee and cigarette smoke exposure. MATERIALS AND METHODS: 64 nanocomposite specimens were prepared and allocated in 8 groups: G1 (SBAP + distilled water), G2 (SBAP + coffee), G3 (SBAP + red wine), G4 (SBAP + cigarette smoke), G5 (distilled water), G6 (coffee), G7 (red wine) and G8 (cigarette smoke). The surface roughness was evaluated in three periods: before and after SBAP and after exposuring to agents tested. The color was evaluated according to CIEL*a*b* parameters using reflection spectrophotometer in two moments: initial and 30 days after the exposure to staining agents. Data were subjected to three-way repeated measures ANOVA and Tukey's test (5%). RESULTS: The results showed a higher surface roughness of the nanocomposite submitted only to the SBAP and those exposed to the SBAP followed by exposure to the coffee or wine solution. The previous application of SBAP followed by cigarette smoke exposure did not increase the roughness of nanocomposite. The SBAP procedure just increased the staining for cigarette smoke group. CONCLUSION: The SBAP increases resin surface roughness, which worsens when there is exposure to coffee and red wine solution. In addition, SBAP may also provide increased staining of nanocomposites exposed to cigarette smoke

Pigmentação de restaurações de resina composta: uma revisão de literatura / Color alteration of composite resins: a literature review

As resinas compostas são materiais restauradores que reproduzem as características dos tecidos dentais. Contudo, frente aos desafios mecânicos e químicos a que são submetidas na cavidade bucal, sofrem efeitos indesejáveis, como pigmentação e manchamento. O objetivo do presente estudo foi revisar a literatura sobre a etiologia da pigmentação da resina composta e expor alternativas clínicas e domiciliares para reduzir os seus efeitos na alteração de cor deste material odontológico estético. Foi realizado um levantamento bibliográfico na base de dados do Pubmed, no período de agosto de 2009 à agosto de 2014 (5 anos). Os termos utilizados foram: “composite resin and color stability”, “composite resin and pigmentation” e “composite resin and color and pigmentation”. Os critérios de inclusão foram estudos clínicos em humanos e estudos in vitro, na língua inglesa, que avaliaram a estabilidade de cor da resina composta direta e indireta. A pigmentação pode ser atribuída a fatores intrínsecos e extrínsecos. Os fatores intrínsecos se referem à descoloração oriunda de componentes do próprio material, enquanto os fatores extrínsecos estão associados aos hábitos e à dieta do individuo, como o consumo de bebidas e alimentos que possuem corante em sua composição. O profissional pode intervir na prevenção ou redução desses manchamentos através de adequada técnica de confecção das restaurações (polimerização, acabamento e polimento adequados) e, na orientação dos pacientes com relação à higiene oral e aos hábitos que interfiram diretamente na estabilidade de cor do material

Composite resins are restorative materials that reproduce dental tissue characteristics. However, this material is constantly exposed to chemical and mechanical challenges in the oral cavity, resulting in pigmentation and staining. The aim of this study was to review the literature regarding the composite resin pigmentation etiology and to propose clinical and home alternatives to prevent this undesirable characteristic. A literature review was conducted in Pubmed database, from August 2009 to August 2014 (5 years). The terms used were: “composite resin and color stability”, “composite resin and pigmentation” and “composite resin and color and pigmentation”. The inclusion criteria were clinical and in vitro studies, in English, that evaluated the color stability of direct and indirect composite resins. The pigmentation can be attributed to intrinsic and extrinsic factors. Intrinsic factors refer to discoloration resultant from components of the material itself, while extrinsic factors are associated to the diet and habits, as the consumption of beverages and foods, which have pigment composition. The professional can intervene in preventing or reducing the pigmentation through proper technique of restorations (proper polymerization, finishing and polishing) and in guiding patients regarding oral hygiene and habits that directly interfere on material color stability

Changes in water sorption and solubility of dental adhesive systems after cigarette smoke.

Aim. To evaluate the effect of cigarette smoke on water sorption and solubility of four adhesive systems. Materials and Methods. Sixteen disks of each adhesive system were prepared (Adper Scotchbond Multipurpose Adhesive (SA); Adper Scotchbond Multipurpose Adhesive System (Adhesive + Primer) (SAP); Adper Single Bond Plus (SB); Adper Easy One (EO)). Specimens were desiccated until a constant mass was obtained and divided into two groups (n = 8). One-half of the specimens were immersed in deionized water, while the other half were also immersed, but with daily exposure to tobacco smoke. After 21 days, disks were measured again and stored in desiccators until constant mass was achieved. Data were calculated according to ISO specifications and statistically analyzed. Results. The tobacco smoke only significantly affected the water sorption and solubility of EO. There were significant differences in both analyses among materials tested. The SB exhibited the highest water sorption, followed by EO, which demonstrated significantly higher solubility values than SB. The SA and SAP showed low water sorption and solubility, and there were no significant differences between the two. Conclusion. Regardless of smoke exposure, both simplified adhesive systems presented an inferior performance that could be related to the complex mixture of components in such versions.