RESUMO
OBJECTIVES: To assess genotoxic and cytotoxic effect of commercially available toothpastes with the different whitening ingredients. MATERIALS AND METHODS: In vivo assessment of cytotoxicity and genotoxicity of whitening toothpastes with different ingredients using a buccal micronucleus cytome assay (BMCyt assay) comprised 199 participants randomly divided into ten groups based on used whitening or control/conventional toothpaste. The exfoliated buccal mucosal cells were collected, stained, and microscopically evaluated at baseline (T0), 30 days (T1), and 60 days (T2) after the beginning of treatment and 30 days after completing treatment (T3). Statistical evaluation was performed by repeated-measures analysis of variance (two-way ANOVA), Tukey's test, and multiple regression analysis. RESULTS: The genotoxic parameters showed no biologically significant changes in any of the observed period for the tested toothpastes, while cytotoxic parameters (number of cells with karyorrhexis and condensed chromatin) showed statistically significant difference (P < 0.05) among evaluation periods for the three peroxide-containing toothpastes. CONCLUSIONS: Peroxide-containing whitening toothpastes exhibit an increase in certain cytotoxic parameters only during the application period, which return to control values after the cessation of application. CLINICAL SIGNIFICANCE: Whitening toothpastes show no genotoxic effect, while peroxide-containing whitening toothpastes may present significant increase of cytotoxicity (measured by the number of karyorrhexis and condensed chromatin) during the application period. However, these changes observed in clinical conditions cannot be considered significant. TRIAL REGISTRATION: ClinicalTrials.gov: NCT04460755.
RESUMO
This cross-sectional study aimed to assess the factors in dentists' opinions related to oral health and the treatment management of the elderly. An online questionnaire-based survey was conducted among the dentist population (n = 463). Respondents were divided depending on whether they attended the geriatric dentistry course during their education, and 15 questions on the Likert scale demonstrated the difference in their attitudes. The majority of respondents (61.9%) agree that dental studies should pay more attention to acquiring sufficient knowledge and skills in the treatment of the elderly, and 56.2% would like to attend a course on that subject. Compared to those who participated in the geriatric dentistry course, those who did not consider providing oral healthcare to older people find it more difficult because of its complexity and practical obstacles (37.3% vs. 54%, p ≤ 0.001). From the results of this study, it can be concluded that there are differences in opinion about the provision of oral healthcare to the elderly between dentists who have and who have not attended a geriatric dentist course during their education. During the dentist's education, geriatric dentistry courses should have a significant role in providing knowledge for working with the elderly population.
