Assuntos
Aterosclerose/diagnóstico , Doenças Cardiovasculares/diagnóstico , Infarto do Miocárdio/diagnóstico , Redes Neurais de Computação , Aterosclerose/patologia , Doenças Cardiovasculares/classificação , Doenças Cardiovasculares/fisiopatologia , Tomada de Decisões , Diagnóstico Diferencial , Humanos , Infarto do Miocárdio/patologiaRESUMO
The content of methicillin resistant S. aureus (MRSA) genes, coding the synthesis of staphylococcal enterotoxins A, B, C (sea, seb, sec) and the toxin of the toxic shock syndrome (tst-H) which was classified with pyrogenic toxins of superantigens (PTSAgs), was studied with the use of PCR amplification. The study revealed the specific features of the content of genes sea and sec, detected in epidemic strains, identified earlier and found to circulate in Russian hospitals. Among the isolates, genetically related to international epidemic strain EMRSA-1, isolates containing no gene sea were detected, while among the isolates genetically related to strain EMRSA-2, isolates containing not only gene sea, but also gene sec were detected, which was indicative of the tendence of this epidemic strain in the direction of further acquisition of pathogenicity genes. As revealed in further studies, among the cultures obtained in bacteriemia, 88% contained gene sea. Two out of three isolates obtained from patients with the symptoms of toxic shock also contained this gene. The differences in the content of genes PTSAgs (sea, seb, sec and tst-H) could serve as a genetic criterium for the differention of isolates circulating in a hospital, as well as for a more complete characterization of the epidemic strains MRSA. The determination of the given genetic markers in genetic strains in circulating strains will make it possible to prognosticate the structure, severity and outcomes of hospital infections. The conditions of PCR amplification for the determination of genes sea, seb, sec and tst-H, as well as multiplex PCR for the determination of genes sea and seb, were developed.
Assuntos
Infecção Hospitalar/epidemiologia , Surtos de Doenças , Infecções Estafilocócicas/epidemiologia , Staphylococcus aureus/genética , Antibacterianos/farmacologia , Bacteriemia/epidemiologia , Toxinas Bacterianas/genética , Farmacorresistência Bacteriana , Enterotoxinas/genética , Marcadores Genéticos/genética , Hospitais , Humanos , Meticilina/farmacologia , Epidemiologia Molecular , Reação em Cadeia da Polimerase , Federação Russa/epidemiologia , Choque Séptico/epidemiologia , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/imunologia , Superantígenos/genéticaRESUMO
A rapid method for measuring phosphatase activity of staphylococci has been developed. Russian 96-well polystyrene plates are used. 0.1 ml of 0.2% sodium phenolphthalein phosphate solution in 1% peptone water, pH 6.8 to 7.0, and 0.1 ml of the tested strain suspension in normal saline are put into wells and incubated for 5 h at 37 degrees C. The results are assessed after adding 0.05 ml of 2.5% ammonia spirit into each well. If the result is positive, the medium is colored raspberry-red, if negative-light-yellow. The method is simple, available, and cheap.
