RESUMO
A procedure for isolating biologically active substances from Galleria mellonella using a culture of isolated giant neurons of mollusc Lymnaea stagnalis as a test-system is described. Fractions capable of activating neurites and inhibiting aggregation of neuronal cells within a range of concentrations from 1 to 30 micrograms/ml were isolated. The fractions obtained have in their chemical composition about 10.5% N, also contain P and S. They have a carbohydrate component.
Assuntos
Produtos Biológicos/farmacologia , Lepidópteros/análise , Lymnaea/metabolismo , Mariposas/análise , Neurônios/citologia , Animais , Produtos Biológicos/isolamento & purificação , Agregação Celular/efeitos dos fármacos , Células Cultivadas , Larva/análise , Lymnaea/efeitos dos fármacos , Neurônios/efeitos dos fármacosRESUMO
The present study was performed to determine the chemical composition and molecular weight of channel-forming complex by fractioning the cell homogenate supernatant by different means (chromatography, gel-electrophoresis, using dissociating and denaturating agents). Availability of the channel-forming complexes in individual fractions is judged from reconstruction of channels in BLM. The Ca-channels reconstructed in BLM are formed by molecules of a protein nature (presumably, peptides). Their molecular weight is no more than 20000 (presumably, 5000). A high stability of spacial organization of channel forming molecules was observed. The channels--subunits aggregate without any change in properties of the selective filter. The thermodynamically preferred agagregates have conductivity of 200 pmho in 0.1 M KCl and seem to consist of 80 channels--subunits which are switched off and on all together.
