[Phyto pharmacological correction of functional state, hormonal status and memory productivity in patients of different ages with organic changes of brain vessels.]

The course intake of the nutritional supplement «Kardisten¼ normalizes increased levels of cortisol in the blood, optimizes the activity of the vessels of the brain, improving the functional state and leading to an increase in the productivity of short-term and long-term memory, more pronounced in the group of persons up to 50. At initial organic changes in the brain vessels, «Kardisten¼ significantly reduces the level of cortisol, increases to a greater extent the productivity of memory than at atherosclerosis. «Kardisten¼ activates the natural mechanisms of increasing the functional capabilities of an organism, exerting a beneficial effect on the body of adults and the elderly people, and can be recommended for the correction of physical, mental health and preservation of active longevity.

[Biochemical parallels of cellular adaptive reactions at chronic low-intensity irradiation and action of phitoecdysteroid preparation serpisten].

Comparison of action of chronic γ-irradiation at a dose of 22.6 cGy and the serpisten substance containing phitoecdysteroids at small doses of 5 and 50 mg/kg on biochemical indicators in erythrocytes and tissues of white not purebred mice is given. It is established that in both cases there is an increase of minor fractions of cardiolipin and phosphatidic acid, lysophosphatidilcholin and a share of phospholipids as part of common lipids. Course administration of serpisten to rats at the total doses of 12 and 30 mg/kg leads to an increase in tissues of thermal shock proteins of family 70 (Hsp70 and Hsc70). Similarity of action of ecdysteroid preparations and the influence of stress factors of physical nature of low intensity (gamma radiation at a small dose) have been detected in mice, which manifest themselves in some chain links of lipid peroxidation processes as well as an increase in biosynthesis of thermal shock proteins of family 70 (Hsp70 and Hsc70) in rats at administration of serpisten.

[Effect of intragastrically injection of phytoecdysteroids on some indicators of hormonal status in rats].

The experiment in vivo in growing male Wistar rats was carried out. The animals of the experimental 2-4 groups were daily intragastrically injected water solutions of the dried extract from the leaves of Seratulla coronata L. in volume of 1.0 ml, containing 2, 20 and 50 mg of phytoecdysteroids per kg of animal weight, accordingly. Animals of control group were daily injected 1.0 ml of water. The content of phytoecdysteroids in the dry extract was analyzed by high performance liquid chromatography (HPLC). The concentration of the sum of phytoecdysteroids in dry extract was 6.15%, 66% of which was 20-hydroxyecdysone and 23% was 25S-inokosteron. On the 15th day animals were taken out of the experiment by the decapitation. The content of corticosterone, prostaglandin E2 and beta-endorphin in rat blood plasma were determined by ELISA test. The pathological--anatomical analysis and weighing of the liver did not reveal any adverse changes of this organ in the animals of all groups. The average concentration of blood plasma corticosterone reduced with increasing of the dose of the extract injected to the animals, reaching significant differences relative to the control group (60.9 +/- 9.4 ng/ml) for 3 and 4 groups (22.7 +/- 6.6 and 17.6 +/- 7.3 ng/ml, accordingly). Beta-endorphin and prostaglandin E2 levels did not differ. The ratio of the mediator of stress (corticosterone) and inhibitors of stress (beta-endorphin and prostaglandin E2) has been calculated. A monotonic decrease of corticosterone/beta-endorphin and corticosterone/prostaglandin E2 ratio has been found with extract dose increasing. Taken together the results of determination of biochemical parameters of the general adaptation syndrome the dose-dependent stress-protective effect of Seratulla coronata L extract has been demonstrated.