RESUMO
Effects of feeder cells on adhesion of human and rat keratinocytes were studied. The human keratinocytes from skin fragments, obtained after surgery and rat keratinocytes from tail skin of young animals, were isolated by cold trypsinization. To evaluate the rate of adhesion the human keratinocytes were labeled in vitro with 14C-leucine while the rat keratinocytes were labeled in vivo with- 14C-thymidine. The human embryo fibroblasts and stromal cells of fetal bone marrow found to be more effective than Swiss 3T3 cells, adult dermal fibroblasts, and A431 epidermoid carcinoma cells in promoting keratinocyte adhesion. The adsorbed collagen supported cell adhesion better than cultural plastic surface but was inferior to feeder cells. The rate of keratinocyte culture formation was dependent on the feeder layer density: the optimum density was 50 x 10(3) cells/cm2. Dynamics of keratinocyte adhesion to substrata was studied.
