[The IgG subclasses in patients with inhibitory form of hemophilia infected and noninfected with viruses].

The sample consisted of 102 patients with hemophilia infected and non-infected with hepatitis viruses. It is established that in case of inhibitory form of hemophilia concentration of IgG increases at the expense of subclass II and in case of non-inhibitory form of hemophilia valuable increase of concentration of IgG occurs at the expense of subclasses I, II and III under concomitant chronic hepatitis. No significant differences between these groups in levels of antibodies to factors VIII and IX is established.

[Effect of cryoconservation of platelets on polyamine level and cathepsin D activity]. / Vliianie kriokonservirovaniia trombotsitov na uroven' poliaminov i aktivnost' katepsina D.

Alterations in activity of cathepsin D as well as in content of polyamines spermine, spermidine, x-fraction (apparently, acetyl spermidine) and putrescine were studied in blood platelets depending on conditions of conservation. The enzyme activity and content of polyamines correlated directly with duration of the cell concentrates storage. Cathepsin D and polyamines appear to be involved in responses to stress adaptation.

[Levels of extracellular DNA and polyamines as criteria of resistance to chemotherapy in patients with acute leukemia]. / Urovni vnekletochnoi DNK i poliaminov kak pokazateli refrakternosti k khimioterapii bol'nykh ostrymi leikozami.

Content of polyamines and extracellular DNA were studied in blood of patients with acute leukoses which did not respond to chemotherapy. Concentration of total polyamines was distinctly lower in blood of patients with acute lymphoblast and myeloblast leukoses as compared with healthy persons. Free and total polyamines were unaltered in blood of these patients after cytostatic therapy. In the patients with acute leukoses content of extracellular DNA was 2-fold higher as compared with controls. The high DNA content was maintained during the therapeutic course. Absence of any alterations in the biochemical patterns studied suggest that the patients with acute leukoses did not respond to the course of treatment used.

[Middle molecules as an indicator of the degree of disruption of blood coagulation in hemophilia]. / Molekuly srednei massy kak pokazatel' stepeni narusheniia gemokoaguliatsii pri gemofilii.

The blood plasma levels of medium-mass molecules (MMM) have been studied by UV spectrophotometry in patients with hemophilia A, B, and AB. The hemocoagulation status has been estimated with the use of the parameters reflecting the general blood coagulation ability and with the correction tests, using the thromboplastin generation test. The MMM levels in all hemophilia forms under study have been found essentially elevated vs. those in health. A clear-cut relationship between the severity of hemostasis disorders, blood plasma MMM levels, and the coagulologic parameters has been demonstrated.

[Significance of polyamines for the early diagnosis of spontaneous hemoblastoses in AKR mice]. / Znachenie poliaminov dlia rannei diagnostiki spontannykh gemoblastozov myshei AKR.

Polyamines levels were studied in various tissue specimens obtained from high-leukemia AKR mice aged 12 months and less. Organ tissues of leukemic animals revealed increased levels of polyamines and changes in their profile. In symptomatic mice, this was matched by relevant morphologic changes. It is inferred that putrescine is a marker of latent leukemia development.