[The adhesive properties and surface characteristics of streptococci colonizing buccal epithelial cells]. / Adgezivnye svoistva i kharakteristika poverkhnosti streptokokkov, koloniziruiushchikh kletki bukkal'nogo épiteliia.

The species spectrum of streptococci subjected to desorption has been found to differ from that of streptococci tightly adhering to epithelial cells, the former streptococci having more hydrophilic surface than the latter ones. No difference in their electrophoretic mobility has been detected. The study of the adhesive properties of the isolated strains has revealed that streptococci with the hydrophilic surface, subjected to desorption in the process of cell washing, less actively adhere to cells of the buccal epithelium.

[Features of the initial stage of surface colonization by Eshcherichia coli cells as a function of their mobility and chemotaxis]. / Osobennosti nachal'noi stadii kolonizatsii poverkhnostei Escherichia coli v zavisimosti ot ikh podvizhnosti i khemotaksisa.

Dependence of adhesion and colonization of hydrophilic and hydrophobic surfaces by Escherichia coli strains with different mobility and chemotaxis was studied using E. coli mot+che+, E. coli mot+che-, E. coli mot-che+. Primary adhesion was shown to correlate with mobility of cells and hydrophilic/hydrophobic character of their surface. Secondary adhesion correlated in addition with chemotactic characteristics of bacteria. E. coli populations were shown to vary in electrophoretic mobility and cells capability for adhesion and chemotaxis.

[The adhesive properties of S and R mutants of Salmonella minnesota during batch cultivation]. / Adgezivnye svoistva S- i R-mutantov Salmonella minnesota v protsesse periodicheskogo kul'tivirovaniia.

The study of the adhesive, surface-electrical and hydrophobic properties of S. minnesota S- and R-mutants in the process batch cultivation has revealed that the maximum values of the above parameters are characteristic of the stationary phase of culture growth and their minimum values, of the end of the lag phase. Electrophoretic mobility and adhesiveness increase from S- to Re-chemotype. The surface of Salmonella S-form is more hydrophilic, than in R-mutants which are indistinguishable in the degree of their hydrophobic properties. The heterogeneity of the populations under study with respect to the electrophoretic mobility and adhesiveness of cells has been detected.

[Heterogeneity of Escherichia coli population during induced autolysis]. / Geterogennost' populiatsii Escherichia coli v protsesse indutsirovannogo avtoliza.

Escherichia coli M-17 autolysis was induced by eliminating nutrition sources from the growth medium and exerting a shock with EDTA. The overall cell number, the optical density of the cell suspension, the number of colony-forming units (CFU), and [3H]uracil incorporation into the cells were analysed in the course of autolysis. The number of CFU was found to drop down faster than the overall cell number in the process of autolysis. The population of E. coli was shown to be heterogeneous in its sensitivity to the induction of autolysis, and some nonlysed cells were still metabolically active. When the rate of autolysis was highest in some cells of the population, the labeled precursor was found to be incorporated into the TCA-soluble and TCA-insoluble fractions of nonlysed cells. The overall cell number, the optical density of the cell suspension, and the number of CFU increased 96 h after the induction of autolysis. The authors discuss what is the role played by the heterogeneity of an E. coli population in its adaptation to EDTA-induced autolysis.

[Heterogeneity of the population of Escherichia coli thr- by assimilation of 3H-threonine]. / Geterogennost' populiatsii Escherichia coli thr- po usvoeniiu 3H-treonina.

The incorporation and spatial localisation of [3H]threonine in the cells of Escherichia coli RP 477 (leu-his-thr-B1-) were studied using the techniques of radiometry and adsorption electron-microscopic autoradiography with additional time-lapse microfilming to follow up the growth of the population. The strain was shown to be heterogeneous in its metabolic, reproductive and morphological (cell diameter) characteristics; a generative cluster was revealed. Indirect data indicated that the so-called "resting" cells were functionally active.

[Heterogeneity of Shigella flexneri during selective synchronization]. / Geterogennost' shigell Fleksnera v protsesse izbiratel'noi sinkhronizatsii.

The study of a number of main growth characteristics of S. flexneri (S) cells during the first and second cycles of their cultivation in a solid culture medium in Peshkov's chamber from seed material, nonsynchronized and synchronized by C. E. Helmstetter's method, has revealed that this method does not permit obtaining completely synchronized cultures. Four physiological cell clusters, differing in the time of generation, growth rate, size at the beginning and the end of the cycle and the time of adaptation, have been detected.

[Structure of a Shigella flexneri population in the process of forming microcolonies]. / Struktura populiatsii Shigella flexneri v protsesse formirovaniia mikrokolonii.

The study of the adaptation period of the S- and R-forms of S. flexneri in a nutritionally sufficient medium has revealed that the population structure characteristic of the S-form and Ra-chemotype comprises two classes of cells: actively growing and nonviable. Besides these two classes, the class of spontaneously lysing bacteria is present in the population of mutants with various degrees of lipopolysaccharide (LPS) lesions. The number of bacteria subject to autolysis increases with the progress of the degradation of the R-nucleus of LPS and depends on age of the inoculate. Morphological analysis has revealed that lysis affects mainly cells 2-3 microns long, which were in the middle of the growth stage at the moment of their inoculation.

[Dissociation and phagolytic characteristics of the Shigella isolated from patients with various clinical forms of dysentery]. / Osobennosti dissotsiatsii i fagolizisa shigell, vydelennykh ot bol'nykh s razlichnymi klinicheskimi formami dizenterii.

The relationship between the severity of the disease and the phagolysis of the isolated cultures of S. flexneri and S. sonnei has been established. In the moderately severe form of the disease the duration of the latent period of the development of the phage has been found to increase in comparison with that in the mild form of the disease.

[Morphological characteristics of phagolysis of S- and R-forms of Shigella flexneri]. / Morfologicheskie osobennosti fagolizisa S- i R-form Shigella flexneri.

The differences in the character and time of the lysis of S. flexneri S- and R-mutant cells have been detected by the method of time-lapse microfilming. The asymmetry of the phagolysis of the S-form of shigellae infected with phage F1 has been revealed.

[Growth dyssymmetry of Shigella flexneri 6(762 S) cells]. / Dissimmetriia rosta kletok Shigella flexneri 6(762 S).

Dyssymmetry in the growth of the initial and secondary shigella cells at early stages of the formation of microcolonies has been revealed by the method of dynamic morphometry in serial photographs obtained by time-lapse microfilming. Electron-microscopic autoradiography has allowed revealing dyssymmetry in the assimilation of exogenous H3-thymidine, H3-threonine, H3-asparaginic acid by different halves of shigella cells in the process of their growth and preparation for mytosis. These results suggest that the shigellae are divided into two unequal cells: the "mother" cell and the "daughter" one.