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1.
Nucleic Acids Res ; 52(8): 4541-4555, 2024 May 08.
Artigo em Inglês | MEDLINE | ID: mdl-38499490

RESUMO

Formation of programmed DNA double-strand breaks is essential for initiating meiotic recombination. Genetic studies on Arabidopsis thaliana and Mus musculus have revealed that assembly of a type IIB topoisomerase VI (Topo VI)-like complex, composed of SPO11 and MTOPVIB, is a prerequisite for generating DNA breaks. However, it remains enigmatic if MTOPVIB resembles its Topo VI subunit B (VIB) ortholog in possessing robust ATPase activity, ability to undergo ATP-dependent dimerization, and activation of SPO11-mediated DNA cleavage. Here, we successfully prepared highly pure A. thaliana MTOPVIB and MTOPVIB-SPO11 complex. Contrary to expectations, our findings highlight that MTOPVIB differs from orthologous Topo VIB by lacking ATP-binding activity and independently forming dimers without ATP. Most significantly, our study reveals that while MTOPVIB lacks the capability to stimulate SPO11-mediated DNA cleavage, it functions as a bona fide DNA-binding protein and plays a substantial role in facilitating the dsDNA binding capacity of the MOTOVIB-SPO11 complex. Thus, we illustrate mechanistic divergence between the MTOPVIB-SPO11 complex and classical type IIB topoisomerases.


Assuntos
Proteínas de Arabidopsis , Arabidopsis , DNA Topoisomerases Tipo II , Trifosfato de Adenosina/metabolismo , Arabidopsis/genética , Arabidopsis/enzimologia , Arabidopsis/metabolismo , Proteínas de Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , Proteínas Arqueais , Quebras de DNA de Cadeia Dupla , DNA Topoisomerases/metabolismo , DNA Topoisomerases/genética , DNA Topoisomerases Tipo II/metabolismo , Proteínas de Ligação a DNA/metabolismo , Proteínas de Ligação a DNA/genética , Endodesoxirribonucleases/metabolismo , Endodesoxirribonucleases/genética , Endodesoxirribonucleases/química , Evolução Molecular , Meiose , Multimerização Proteica
2.
Nat Plants ; 9(12): 2016-2030, 2023 12.
Artigo em Inglês | MEDLINE | ID: mdl-37973938

RESUMO

The synaptonemal complex (SC) is a proteinaceous structure that forms between homologous chromosomes during meiosis prophase. The SC is widely conserved across species, but its structure and roles during meiotic recombination are still debated. While the SC central region is made up of transverse filaments and central element proteins in mammals and fungi, few central element proteins have been identified in other species. Here we report the identification of two coiled-coil proteins, SCEP1 and SCEP2, that form a complex and localize at the centre of the Arabidopsis thaliana SC. In scep1 and scep2 mutants, chromosomes are aligned but not synapsed (the ZYP1 transverse filament protein is not loaded), crossovers are increased compared with the wild type, interference is lost and heterochiasmy is strongly reduced. We thus report the identification of two plant SC central elements, and homologues of these are found in all major angiosperm clades.


Assuntos
Proteínas de Arabidopsis , Arabidopsis , Animais , Complexo Sinaptonêmico/genética , Complexo Sinaptonêmico/metabolismo , Prófase , Arabidopsis/genética , Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Meiose , Mamíferos/genética
3.
Genetics ; 221(4)2022 07 30.
Artigo em Inglês | MEDLINE | ID: mdl-35666201

RESUMO

The principles of heredity state that the two alleles carried by a heterozygote are equally transmitted to the progeny. However, genomic regions that escape this rule have been reported in many organisms. It is notably the case of genetic loci referred to as gamete killers, where one allele enhances its transmission by causing the death of the gametes that do not carry it. Gamete killers are of great interest, particularly to understand mechanisms of evolution and speciation. Although being common in plants, only a few, all in rice, have so far been deciphered to the causal genes. Here, we studied a pollen killer found in hybrids between two accessions of Arabidopsis thaliana. Exploring natural variation, we observed this pollen killer in many crosses within the species. Genetic analyses revealed that three genetically linked elements are necessary for pollen killer activity. Using mutants, we showed that this pollen killer works according to a poison-antidote model, where the poison kills pollen grains not producing the antidote. We identified the gene encoding the antidote, a chimeric protein addressed to mitochondria. De novo genomic sequencing in 12 natural variants with different behaviors regarding the pollen killer revealed a hyper variable locus, with important structural variations particularly in killer genotypes, where the antidote gene recently underwent duplications. Our results strongly suggest that the gene has newly evolved within A. thaliana. Finally, we identified in the protein sequence polymorphisms related to its antidote activity.


Assuntos
Arabidopsis , Venenos , Alelos , Antídotos/metabolismo , Arabidopsis/genética , Arabidopsis/metabolismo , Venenos/metabolismo , Pólen/genética
4.
Nucleic Acids Res ; 49(17): 9821-9835, 2021 09 27.
Artigo em Inglês | MEDLINE | ID: mdl-34458909

RESUMO

In the current meiotic recombination initiation model, the SPO11 catalytic subunits associate with MTOPVIB to form a Topoisomerase VI-like complex that generates DNA double strand breaks (DSBs). Four additional proteins, PRD1/AtMEI1, PRD2/AtMEI4, PRD3/AtMER2 and the plant specific DFO are required for meiotic DSB formation. Here we show that (i) MTOPVIB and PRD1 provide the link between the catalytic sub-complex and the other DSB proteins, (ii) PRD3/AtMER2, while localized to the axis, does not assemble a canonical pre-DSB complex but establishes a direct link between the DSB-forming and resection machineries, (iii) DFO controls MTOPVIB foci formation and is part of a divergent RMM-like complex including PHS1/AtREC114 and PRD2/AtMEI4 but not PRD3/AtMER2, (iv) PHS1/AtREC114 is absolutely unnecessary for DSB formation despite having a conserved position within the DSB protein network and (v) MTOPVIB and PRD2/AtMEI4 interact directly with chromosome axis proteins to anchor the meiotic DSB machinery to the axis.


Assuntos
Proteínas de Arabidopsis/metabolismo , Arabidopsis/genética , Quebras de DNA de Cadeia Dupla , Meiose/genética , Proteínas de Arabidopsis/fisiologia , Proteínas de Ciclo Celular/metabolismo , Cromatina/metabolismo , Reparo do DNA , Proteínas de Ligação a DNA/metabolismo , Proteínas Tirosina Fosfatases/fisiologia , Recombinação Genética
5.
Plant J ; 95(2): 385-396, 2018 07.
Artigo em Inglês | MEDLINE | ID: mdl-29681056

RESUMO

During meiotic prophase I chromosomes undergo dramatic conformational changes that accompany chromosome condensation, pairing and recombination between homologs. These changes include the anchoring of telomeres to the nuclear envelope and their clustering to form a bouquet. In plants, these events have been studied and illustrated in intact meiocytes of species with large genomes. Arabidopsis thaliana is an excellent genetic model in which major molecular pathways that control synapsis and recombination between homologs have been uncovered. Yet the study of chromosome dynamics is hampered by current cytological methods that disrupt the three-dimensional (3D) architecture of the nucleus. Here we set up a protocol to preserve the 3D configuration of A. thaliana meiocytes. We showed that this technique is compatible with the use of a variety of antibodies that label structural and recombination proteins and were able to highlight the presence of clustered synapsis initiation centers at the nuclear periphery. By using fluorescence in situ hybridization we also studied the behavior of chromosomes during pre-meiotic G2 and prophase I, revealing the existence of a telomere bouquet during A. thaliana male meiosis. In addition we showed that the number of telomeres in a bouquet and its volume vary greatly, thus revealing the complexity of telomere behavior during meiotic prophase I. Finally, by using probes that label subtelomeric regions of individual chromosomes, we revealed differential localization behaviors of chromosome ends. Our protocol opens new areas of research for investigating chromosome dynamics in A. thaliana meiocytes.


Assuntos
Arabidopsis/genética , Cromossomos de Plantas/genética , Meiose/genética , Recombinação Genética/genética , Imageamento Tridimensional/métodos , Prófase , Telômero/metabolismo
6.
Semin Cell Dev Biol ; 54: 165-76, 2016 06.
Artigo em Inglês | MEDLINE | ID: mdl-26995551

RESUMO

Meiotic recombination is initiated by the formation of programmed DNA double-strand breaks (DSBs). More than 15 years ago, Spo11 was identified as the protein responsible for meiotic DSB formation, notably because of its striking similarities with the A subunit of topoisomerase VI (TopoVI). TopoVI are enzymes that modify DNA topology by generating transient DSBs and are active as heterotetramers, composed of two A and two B subunits. A2 dimers catalyse the DNA cleavage reaction, whereas the B subunits regulate A2 conformation, DNA capture, cleavage and re-ligation. The recent identification in plants and mammals of a B-like TopoVI subunit that interacts with SPO11 and is required for meiotic DSB formation makes us to reconsider our understanding of the meiotic DSB catalytic complex. We provide here an overview of the knowledge on TopoVI structure and mode of action and we compare them with their meiotic counterparts. This allows us to discuss the nature, structure and functions of the meiotic TopoVI-like complex during meiotic DSB formation.


Assuntos
Biocatálise , Quebras de DNA de Cadeia Dupla , Enzimas/metabolismo , Meiose , Endodesoxirribonucleases/metabolismo , Modelos Biológicos
7.
Science ; 351(6276): 939-43, 2016 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-26917763

RESUMO

The SPO11 protein catalyzes the formation of meiotic DNA double strand breaks (DSBs) and is homologous to the A subunit of an archaeal topoisomerase (topo VI). Topo VI are heterotetrameric enzymes comprising two A and two B subunits; however, no topo VIB involved in meiotic recombination had been identified. We characterized a structural homolog of the archaeal topo VIB subunit [meiotic topoisomerase VIB-like (MTOPVIB)], which is essential for meiotic DSB formation. It forms a complex with the two Arabidopsis thaliana SPO11 orthologs required for meiotic DSB formation (SPO11-1 and SPO11-2) and is absolutely required for the formation of the SPO11-1/SPO11-2 heterodimer. These findings suggest that the catalytic core complex responsible for meiotic DSB formation in eukaryotes adopts a topo VI-like structure.


Assuntos
Proteínas Arqueais/química , DNA Topoisomerases Tipo II/química , Endodesoxirribonucleases/química , Recombinação Homóloga , Meiose/genética , Methanosarcina/enzimologia , Sulfolobus/enzimologia , Sequência de Aminoácidos , Arabidopsis/enzimologia , Proteínas de Arabidopsis/química , Proteínas de Arabidopsis/genética , Proteínas Arqueais/genética , Catálise , Domínio Catalítico , Quebras de DNA de Cadeia Dupla , DNA Topoisomerases/química , DNA Topoisomerases/genética , DNA Topoisomerases Tipo II/genética , Endodesoxirribonucleases/genética , Modelos Moleculares , Dados de Sequência Molecular , Multimerização Proteica , Estrutura Secundária de Proteína , Saccharomyces cerevisiae/enzimologia , Alinhamento de Sequência , Homologia Estrutural de Proteína , Técnicas do Sistema de Duplo-Híbrido
8.
Plant Physiol ; 170(1): 354-66, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26537562

RESUMO

Mitochondrial translation involves a complex interplay of ancient bacteria-like features and host-derived functionalities. Although the basic components of the mitochondrial translation apparatus have been recognized, very few protein factors aiding in recruiting ribosomes on mitochondria-encoded messenger RNA (mRNAs) have been identified in higher plants. In this study, we describe the identification of the Arabidopsis (Arabidopsis thaliana) MITOCHONDRIAL TRANSLATION FACTOR1 (MTL1) protein, a new member of the Pentatricopeptide Repeat family, and show that it is essential for the translation of the mitochondrial NADH dehydrogenase subunit7 (nad7) mRNA. We demonstrate that mtl1 mutant plants fail to accumulate the Nad7 protein, even though the nad7 mature mRNA is produced and bears the same 5' and 3' extremities as in wild-type plants. We next observed that polysome association of nad7 mature mRNA is specifically disrupted in mtl1 mutants, indicating that the absence of Nad7 results from a lack of translation of nad7 mRNA. These findings illustrate that mitochondrial translation requires the intervention of gene-specific nucleus-encoded PPR trans-factors and that their action does not necessarily involve the 5' processing of their target mRNA, as observed previously. Interestingly, a partial decrease in nad7 intron 2 splicing was also detected in mtl1 mutants, suggesting that MTL1 is also involved in group II intron splicing. However, this second function appears to be less essential for nad7 expression than its role in translation. MTL1 will be instrumental to understand the multifunctionality of PPR proteins and the mechanisms governing mRNA translation and intron splicing in plant mitochondria.


Assuntos
Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , NADH Desidrogenase/genética , Splicing de RNA , Arabidopsis/genética , Arabidopsis/crescimento & desenvolvimento , Arabidopsis/metabolismo , Regulação da Expressão Gênica de Plantas , Íntrons , Mitocôndrias/genética , Mitocôndrias/metabolismo , Mutação , NADH Desidrogenase/metabolismo , Plantas Geneticamente Modificadas , Polirribossomos/genética , Polirribossomos/metabolismo , Biossíntese de Proteínas , RNA Mensageiro/genética , RNA Mitocondrial
9.
Nucleic Acids Res ; 42(19): 11965-78, 2014 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-25260587

RESUMO

The eukaryotic RecA homologue Rad51 is a key factor in homologous recombination and recombinational repair. Rad51-like proteins have been identified in yeast (Rad55, Rad57 and Dmc1), plants and vertebrates (RAD51B, RAD51C, RAD51D, XRCC2, XRCC3 and DMC1). RAD51 and DMC1 are the strand-exchange proteins forming a nucleofilament for strand invasion, however, the function of the paralogues in the process of homologous recombination is less clear. In yeast the two Rad51 paralogues, Rad55 and Rad57, have been shown to be involved in somatic and meiotic HR and they are essential to the formation of the Rad51/DNA nucleofilament counterbalancing the anti-recombinase activity of the SRS2 helicase. Here, we examined the role of RAD51B in the model bryophyte Physcomitrella patens. Mutant analysis shows that RAD51B is essential for the maintenance of genome integrity, for resistance to DNA damaging agents and for gene targeting. Furthermore, we set up methods to investigate meiosis in Physcomitrella and we demonstrate that the RAD51B protein is essential for meiotic homologous recombination. Finally, we show that all these functions are independent of the SRS2 anti-recombinase protein, which is in striking contrast to what is found in budding yeast where the RAD51 paralogues are fully dependent on the SRS2 anti-recombinase function.


Assuntos
Bryopsida/genética , Recombinação Homóloga , Meiose/genética , Proteínas de Plantas/fisiologia , Rad51 Recombinase/fisiologia , Bryopsida/anatomia & histologia , Bryopsida/efeitos dos fármacos , Bryopsida/crescimento & desenvolvimento , Dano ao DNA , DNA Helicases/genética , DNA Helicases/fisiologia , Deleção de Genes , Fenótipo , Proteínas de Plantas/genética , Rad51 Recombinase/genética
10.
Nucleic Acids Res ; 41(13): 6650-63, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-23658225

RESUMO

Gene expression in plant mitochondria involves a complex collaboration of transcription initiation and termination, as well as subsequent mRNA processing to produce mature mRNAs. In this study, we describe the function of the Arabidopsis mitochondrial stability factor 1 (MTSF1) gene and show that it encodes a pentatricopeptide repeat protein essential for the 3'-processing of mitochondrial nad4 mRNA and its stability. The nad4 mRNA is highly destabilized in Arabidopsis mtsf1 mutant plants, which consequently accumulates low amounts of a truncated form of respiratory complex I. Biochemical and genetic analyses demonstrated that MTSF1 binds with high affinity to the last 20 nucleotides of nad4 mRNA. Our data support a model for MTSF1 functioning in which its association with the last nucleotides of the nad4 3' untranslated region stabilizes nad4 mRNA. Additionally, strict conservation of the MTSF1-binding sites strongly suggests that the protective function of MTSF1 on nad4 mRNA is conserved in dicots. These results demonstrate that the mRNA stabilization process initially identified in plastids, whereby proteins bound to RNA extremities constitute barriers to exoribonuclease progression occur in plant mitochondria to protect and concomitantly define the 3' end of mature mitochondrial mRNAs. Our study also reveals that short RNA molecules corresponding to pentatricopeptide repeat-binding sites accumulate also in plant mitochondria.


Assuntos
Proteínas de Arabidopsis/metabolismo , Arabidopsis/genética , Complexo I de Transporte de Elétrons/genética , Proteínas Mitocondriais/metabolismo , Processamento de Terminações 3' de RNA , Estabilidade de RNA , RNA Mensageiro/metabolismo , Proteínas de Ligação a RNA/metabolismo , Arabidopsis/crescimento & desenvolvimento , Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , Sítios de Ligação , Respiração Celular , Complexo I de Transporte de Elétrons/metabolismo , Regulação da Expressão Gênica de Plantas , Mitocôndrias/metabolismo , Proteínas Mitocondriais/genética , Mutação , Fotossíntese , Splicing de RNA , Proteínas de Ligação a RNA/genética
11.
PLoS Genet ; 9(1): e1003165, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23300481

RESUMO

Mini-chromosome maintenance (MCM) 2-9 proteins are related helicases. The first six, MCM2-7, are essential for DNA replication in all eukaryotes. In contrast, MCM8 is not always conserved in eukaryotes but is present in Arabidopsis thaliana. MCM8 is required for 95% of meiotic crossovers (COs) in Drosophila and is essential for meiosis completion in mouse, prompting us to study this gene in Arabidopsis meiosis. Three allelic Atmcm8 mutants showed a limited level of chromosome fragmentation at meiosis. This defect was dependent on programmed meiotic double-strand break (DSB) formation, revealing a role for AtMCM8 in meiotic DSB repair. In contrast, CO formation was not affected, as shown both genetically and cytologically. The Atmcm8 DSB repair defect was greatly amplified in the absence of the DMC1 recombinase or in mutants affected in DMC1 dynamics (sds, asy1). The Atmcm8 fragmentation defect was also amplified in plants heterozygous for a mutation in either recombinase, DMC1 or RAD51. Finally, in the context of absence of homologous chromosomes (i.e. haploid), mutation of AtMCM8 also provoked a low level of chromosome fragmentation. This fragmentation was amplified by the absence of DMC1 showing that both MCM8 and DMC1 can promote repair on the sister chromatid in Arabidopsis haploids. Altogether, this establishes a role for AtMCM8 in meiotic DSB repair, in parallel to DMC1. We propose that MCM8 is involved with RAD51 in a backup pathway that repairs meiotic DSB without giving CO when the major pathway, which relies on DMC1, fails.


Assuntos
Proteínas de Arabidopsis/genética , Arabidopsis/genética , Proteínas de Ciclo Celular , DNA Helicases/genética , Meiose/genética , Recombinases Rec A , Recombinação Genética , Proteínas de Arabidopsis/metabolismo , Proteínas de Ciclo Celular/genética , Proteínas de Ciclo Celular/metabolismo , Cromátides/genética , Troca Genética , Quebras de DNA de Cadeia Dupla , Reparo do DNA/genética , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/metabolismo , Mutação , Rad51 Recombinase/genética , Rad51 Recombinase/metabolismo , Recombinases Rec A/genética , Recombinases Rec A/metabolismo , Recombinases/genética , Recombinases/metabolismo
12.
PLoS Genet ; 8(7): e1002799, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22844245

RESUMO

In numerous species, the formation of meiotic crossovers is largely under the control of a group of proteins known as ZMM. Here, we identified a new ZMM protein, HEI10, a RING finger-containing protein that is well conserved among species. We show that HEI10 is structurally and functionally related to the yeast Zip3 ZMM and that it is absolutely required for class I crossover (CO) formation in Arabidopsis thaliana. Furthermore, we show that it is present as numerous foci on the chromosome axes and the synaptonemal complex central element until pachytene. Then, from pachytene to diakinesis, HEI10 is retained at a limited number of sites that correspond to class I COs, where it co-localises with MLH1. Assuming that HEI10 early staining represents an early selection of recombination intermediates to be channelled into the ZMM pathway, HEI10 would therefore draw a continuity between early chosen recombination intermediates and final class I COs.


Assuntos
Arabidopsis/genética , Troca Genética , Miose/genética , Homologia de Sequência de Aminoácidos , Complexo Sinaptonêmico/genética , Adenosina Trifosfatases/genética , Sequência de Aminoácidos , Arabidopsis/citologia , Proteínas de Arabidopsis/genética , Proteínas Cromossômicas não Histona/genética , Cromossomos de Plantas/genética , Fertilidade/genética , Recombinação Homóloga , Dados de Sequência Molecular , Proteína 1 Homóloga a MutL , Mutação , Domínios RING Finger/genética , Proteínas de Saccharomyces cerevisiae/genética , Ubiquitina-Proteína Ligases/genética , Leveduras/genética
13.
Nucleic Acids Res ; 40(8): 3496-510, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22210882

RESUMO

The moss Physcomitrella patens is unique among plant models for the high frequency with which targeted transgene insertion occurs via homologous recombination. Transgene integration is believed to utilize existing machinery for the detection and repair of DNA double-strand breaks (DSBs). We undertook targeted knockout of the Physcomitrella genes encoding components of the principal sensor of DNA DSBs, the MRN complex. Loss of function of PpMRE11 or PpRAD50 strongly and specifically inhibited gene targeting, whilst rates of untargeted transgene integration were relatively unaffected. In contrast, disruption of the PpNBS1 gene retained the wild-type capacity to integrate transforming DNA efficiently at homologous loci. Analysis of the kinetics of DNA-DSB repair in wild-type and mutant plants by single-nucleus agarose gel electrophoresis revealed that bleomycin-induced fragmentation of genomic DNA was repaired at approximately equal rates in each genotype, although both the Ppmre11 and Pprad50 mutants exhibited severely restricted growth and development and enhanced sensitivity to UV-B and bleomycin-induced DNA damage, compared with wild-type and Ppnbs1 plants. This implies that while extensive DNA repair can occur in the absence of a functional MRN complex; this is unsupervised in nature and results in the accumulation of deleterious mutations incompatible with normal growth and development.


Assuntos
Bryopsida/genética , Quebras de DNA de Cadeia Dupla , Reparo do DNA , Proteínas de Ligação a DNA/fisiologia , Marcação de Genes , Proteínas de Plantas/fisiologia , Bryopsida/crescimento & desenvolvimento , Reparo do DNA/genética , Proteínas de Ligação a DNA/genética , Expressão Gênica , Técnicas de Inativação de Genes , Dados de Sequência Molecular , Mutação , Fenótipo , Proteínas de Plantas/genética
14.
Behav Ther ; 41(4): 575-86, 2010 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-21035620

RESUMO

The present study examined the impact of different modes of processing anxious apprehension on subsequent anxiety and performance in a stressful speech task. Participants were informed that they would have to give a speech on a difficult topic while being videotaped and evaluated on their performance. They were then randomly assigned to one of three conditions. In a specific processing condition, they were encouraged to explore in detail all the specific aspects (thoughts, emotions, sensations) they experienced while anticipating giving the speech; in a general processing condition, they had to focus on the generic aspects that they would typically experience during anxious anticipation; and in a control, no-processing condition, participants were distracted. Results revealed that at the end of the speech, participants in the specific processing condition reported less anxiety than those in the two other conditions. They were also evaluated by judges to have performed better than those in the control condition, who in turn did better than those in the general processing condition.


Assuntos
Ansiedade/psicologia , Ansiedade/terapia , Cognição , Desempenho Psicomotor , Fala , Estresse Psicológico/psicologia , Adolescente , Adulto , Feminino , Humanos , Masculino , Psicoterapia/métodos
15.
Plant Mol Biol ; 70(1-2): 123-37, 2009 May.
Artigo em Inglês | MEDLINE | ID: mdl-19199092

RESUMO

The Ogura cytoplasmic male sterility causing protein, ORF138, was found to be part of a complex with an apparent size of over 750 kDa in the inner membrane of mitochondria of sterile plants. ORF138 did not colocalize with any of the oxidative phosphorylation complexes, nor did its presence modify their apparent size or amount, compared to samples from fertile isogenic plants. We attempted to detect potential proteins or nucleic acids that could be involved in the large ORF138 complex by 2D PAGE, immunoprecipitation and nuclease treatments of native extracts. All our results suggest that the ORF138 protein is the main, if not only, component of this large complex. The capacities of complexes I, II, IV, and ATP synthase were identical in samples from sterile and fertile plants. Isolated mitochondria from sterile plants showed a higher oxygen consumption than those from fertile plants. In vivo respiration measurements suggest that the difference in O(2) consumption measured at the organelle level is compensated at the cell/tissue level, completely in leaves, but only partially in male reproductive organs.


Assuntos
Brassica rapa/metabolismo , Mitocôndrias/metabolismo , Proteínas Mitocondriais/metabolismo , Infertilidade das Plantas , Proteínas de Plantas/metabolismo , Brassica rapa/genética , Eletroforese em Gel de Poliacrilamida , Proteínas Mitocondriais/genética , Fosforilação Oxidativa , Consumo de Oxigênio , Proteínas de Plantas/genética
16.
J Behav Ther Exp Psychiatry ; 40(2): 274-82, 2009 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-19168173

RESUMO

The present study investigates the emotional impact of a specific vs. generic mode of information processing during imaginal exposure. Forty-nine socially anxious students mentally relived a personally experienced stressful social situation. Half of the participants were instructed to activate generic elements of the target event (i.e., elements repetitively experienced in similar social situations); the other half had to recall the specific features of the target event. Results showed that participants who focused on specific elements reported less distress than those who focused on generic features. Clinical implications are discussed.


Assuntos
Emoções/fisiologia , Imaginação/fisiologia , Processos Mentais/fisiologia , Transtornos Fóbicos/psicologia , Meio Social , Adolescente , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Escalas de Graduação Psiquiátrica , Autoimagem , Adulto Jovem
17.
J Exp Bot ; 59(11): 3087-98, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-18583349

RESUMO

The organization and dynamics of the plant endomembrane system require both universal and plant-specific molecules and compartments. The latter, despite the growing wealth of information, remains poorly understood. From the study of an Arabidopsis thaliana male gametophytic mutant, it was possible to isolate a gene named POKY POLLEN TUBE (POK) essential for pollen tube tip growth. The similarity between the predicted POK protein sequence and yeast Vps52p, a subunit from the GARP/VFT complex which is involved in the docking of vesicles from the prevacuolar compartment to the Golgi apparatus, suggested that the POK protein plays a role in plant membrane trafficking. Genetic analysis of Arabidopsis mutants affecting AtVPS53 or AtVPS54 genes which encode putative POK partners shows a transmission defect through the male gametophyte for all lines, which is similar to the pok mutant. Using a combination of biochemical approaches and specific antiserum it has been demonstrated that the POK protein is present in phylogenetically divergent plant species, associated with membranes and belongs to a high molecular weight complex. Combination of immunolocalization studies and pharmacological approaches in different plant cells revealed that the POK protein associates with Golgi and post-Golgi compartments. The role of POK in post-Golgi endomembrane trafficking and as a member of a putative plant GARP/VFT complex is discussed.


Assuntos
Proteínas de Arabidopsis/metabolismo , Arabidopsis/metabolismo , Flores/metabolismo , Complexo de Golgi/metabolismo , Membranas Intracelulares/metabolismo , Proteínas de Transporte Vesicular/metabolismo , Arabidopsis/genética , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/imunologia , Imuno-Histoquímica , Microscopia Confocal , Complexos Multiproteicos/metabolismo , Mutação , Proteínas de Saccharomyces cerevisiae/genética , Proteínas de Transporte Vesicular/genética , Proteínas de Transporte Vesicular/imunologia
18.
PLoS Genet ; 3(10): 1894-906, 2007 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-17937504

RESUMO

During meiosis, homologous chromosomes recognize each other, align, and exchange genetic information. This process requires the action of RecA-related proteins Rad51 and Dmc1 to catalyze DNA strand exchanges. The Mnd1-Hop2 complex has been shown to assist in Dmc1-dependent processes. Furthermore, higher eukaryotes possess additional RecA-related proteins, like XRCC3, which are involved in meiotic recombination. However, little is known about the functional interplay between these proteins during meiosis. We investigated the functional relationship between AtMND1, AtDMC1, AtRAD51, and AtXRCC3 during meiosis in Arabidopsis thaliana. We demonstrate the localization of AtMND1 to meiotic chromosomes, even in the absence of recombination, and show that AtMND1 loading depends exclusively on AHP2, the Arabidopsis Hop2 homolog. We provide evidence of genetic interaction between AtMND1, AtDMC1, AtRAD51, and AtXRCC3. In vitro assays suggest that this functional link is due to direct interaction of the AtMND1-AHP2 complex with AtRAD51 and AtDMC1. We show that AtDMC1 foci accumulate in the Atmnd1 mutant, but are reduced in number in Atrad51 and Atxrcc3 mutants. This study provides the first insights into the functional differences of AtRAD51 and AtXRCC3 during meiosis, demonstrating that AtXRCC3 is dispensable for AtDMC1 focus formation in an Atmnd1 mutant background, whereas AtRAD51 is not. These results clarify the functional interactions between key players in the strand exchange processes during meiotic recombination. Furthermore, they highlight a direct interaction between MND1 and RAD51 and show a functional divergence between RAD51 and XRCC3.


Assuntos
Proteínas de Arabidopsis/genética , Arabidopsis/genética , Meiose , Fosfotransferases/genética , Recombinases Rec A/genética , Cromatina/metabolismo , Dimerização , Regulação da Expressão Gênica de Plantas , Genes de Plantas , Vetores Genéticos , Modelos Genéticos , Dados de Sequência Molecular , Mutação , Recombinação Genética , Análise de Sequência de DNA
19.
J Clin Child Adolesc Psychol ; 36(1): 95-105, 2007 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-17206885

RESUMO

Overgeneral autobiographical memory seems to be a stable cognitive marker in depressed adults and may predict persistence of depression. This study investigated whether depressive disorders in children are associated with overgeneral memory. Sixty children (ages 9 to 13 years) participated; 15 were diagnosed with lifetime depressive disorder, 25 had other lifetime psychiatric disorders, and 20 had no history of psychiatric disorder. Depressed children gave fewer specific memories compared to children with no or other psychiatric disorders, even after controlling for depressive mood, potential traumatic life events, verbal IQ, and verbal memory.


Assuntos
Autobiografias como Assunto , Transtorno Depressivo/epidemiologia , Transtornos da Memória/epidemiologia , Adolescente , Criança , Transtorno Depressivo/diagnóstico , Transtorno Depressivo/psicologia , Feminino , Humanos , Acontecimentos que Mudam a Vida , Masculino , Transtornos da Memória/diagnóstico , Índice de Gravidade de Doença , Transtornos de Estresse Pós-Traumáticos/diagnóstico , Transtornos de Estresse Pós-Traumáticos/epidemiologia , Transtornos de Estresse Pós-Traumáticos/psicologia , Comportamento Verbal , Escalas de Wechsler
20.
J Cell Sci ; 118(Pt 20): 4621-32, 2005 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-16176934

RESUMO

The success of the first meiotic division relies (among other factors) on the formation of bivalents between homologous chromosomes, the monopolar orientation of the sister kinetochores at metaphase I and the maintenance of centromeric cohesion until the onset of anaphase II. The meiotic cohesin subunit, Rec8 has been reported to be one of the key players in these processes, but its precise role in kinetochore orientation is still under debate. By contrast, much less is known about the other non-SMC cohesin subunit, Scc3. We report the identification and the characterisation of AtSCC3, the sole Arabidopsis homologue of Scc3. The detection of AtSCC3 in mitotic cells, the embryo lethality of a null allele Atscc3-2, and the mitotic defects of the weak allele Atscc3-1 suggest that AtSCC3 is required for mitosis. AtSCC3 was also detected in meiotic nuclei as early as interphase, and bound to the chromosome axis from early leptotene through to anaphase I. We show here that both AtREC8 and AtSCC3 are necessary not only to maintain centromere cohesion at anaphase I, but also for the monopolar orientation of the kinetochores during the first meiotic division. We also found that AtREC8 is involved in chromosome axis formation in an AtSPO11-1-independent manner. Finally, we provide evidence for a role of AtSPO11-1 in the stability of the cohesin complex.


Assuntos
Proteínas de Arabidopsis/metabolismo , Arabidopsis/citologia , Arabidopsis/metabolismo , Proteínas de Ciclo Celular/metabolismo , Polaridade Celular , Cinetocoros/metabolismo , Meiose , Anáfase , Arabidopsis/genética , Proteínas de Arabidopsis/genética , Proteínas Cromossômicas não Histona , Cromossomos de Plantas/metabolismo , Proteínas de Ligação a DNA/metabolismo , Proteínas Fúngicas/metabolismo , Genoma de Planta , Mutação/genética , Proteínas Nucleares/metabolismo , Fenótipo , Transporte Proteico , Rad51 Recombinase/metabolismo , Recombinação Genética , Coesinas
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