RESUMO
Evolutionary conservative chemosignal 2,5-dimethylpyrazin that is pheromone in female mice has been shown to increase frequency of mitotic aberrations analyzed with aid of metaphasic and ana-telophasic analysis in bone marrow cells. Replacement of one of methyl radicals in the pheromone molecule by the carboxyl radical reveals specificity of action of the used derivative: the frequency of disturbances revealed only by the ana-telophasic analysis increases, whereas by the metaphasic analysis, no induction of disturbance is detected. In the sperm head abnormality test there is shown a rise of the anomalies by both compounds. Possible mechanisms of specific action of the tested substances on stability of genetic apparatus of the bone marrow dividing cells in the house mouse are discussed.
Assuntos
Células da Medula Óssea/metabolismo , Pirazinas/efeitos adversos , Pirazinas/química , Transdução de Sinais/efeitos dos fármacos , Cabeça do Espermatozoide/metabolismo , Testículo/metabolismo , Animais , Células da Medula Óssea/patologia , Feminino , Masculino , Camundongos , Pirazinas/farmacologia , Atrativos Sexuais/efeitos adversos , Atrativos Sexuais/química , Atrativos Sexuais/farmacologia , Cabeça do Espermatozoide/patologia , Telófase/efeitos dos fármacos , Testículo/patologiaRESUMO
There was studied effect of female house mouse pheromone 2,5-dimethylpyrazine and other pyrazine-containing substances on the genetic apparatus stability of dividing bone marrow cells of male mice of the strain CBA. Differences in action of the used chemosignals are revealed. Role of the method of action on induction of analyzed mitotic aberrations is shown. Spectrum of the aberrations is analyzed. Dependence of cytogenetic activity of the used substances on their structural peculiarities and significance of the revealed effects are discussed.
