RESUMO
Campylobacter is regarded as the most common bacterial cause of gastroenteritis throughout the world and most cases of human campylobacteriosis can be traced back to the consumption of poultry meat. In Brazil, few studies evaluated the genetic relatedness among Campylobacter isolates. The aim of this research was to evaluate the genetic diversity of Campylobacter spp. isolated from poultry meat products sold on the retail market in Southern Brazil. The presumptive identification of Campylobacter was performed using traditional microbiological analysis, followed by molecular confirmation by PCR. The genetic diversity of isolates was analyzed by pulsed-field gel electrophoresis (PFGE). Campylobacter spp. was isolated from 91.7% (33/36) of the samples, totaling 48 isolates. Campylobacter jejuni was the most prevalent species isolated (90.8%). PFGE data revealed 26 pulsotypes and 18 PFGE patterns composed of only 1 isolate. Campylobacter isolates exhibited high genetic diversity; however, some clones were recurrent in the poultry meat products sold on the retail market. As the south region of Brazil is an important producer and exporter of chicken meat, our results highlight the need to control this pathogen in the food chain in this area of the world to reduce the risks of exposing consumers to campylobacteriosis.
Assuntos
Campylobacter coli/genética , Campylobacter jejuni/genética , Microbiologia de Alimentos , Variação Genética , Produtos Avícolas/microbiologia , Animais , Brasil , Campylobacter coli/isolamento & purificação , Campylobacter jejuni/isolamento & purificação , Galinhas , Eletroforese em Gel de Campo Pulsado/veterinária , Reação em Cadeia da Polimerase/veterináriaRESUMO
With 44.9 % trauma is the main cause of death in men under 40 years. From September 3(rd) 1997 to June 30(th) 1998 174 patients with severe injuries or polytrauma were treated in our hospital. The mean ISS was 29 (18-75). 15 (8.75 %) patients died within the first 24 hours. The mean age was 34.5 (2-85) years. The mean ISS of these patients was 48 (25-75). In a retrospective study we analyzed the pattern of injury as well as the preclinical and clinical management. The fact that orthopedic surgery still has got a poor position within the patient's satisfaction-scale and that 15 of 174 analyzed patients had to die within the first 24 hours points out the importance of internal and external quality improvement measurements.
Assuntos
Causas de Morte , Mortalidade Hospitalar , Traumatismo Múltiplo/mortalidade , Adolescente , Adulto , Idoso , Berlim , Criança , Pré-Escolar , Serviços Médicos de Emergência , Feminino , Humanos , Escala de Gravidade do Ferimento , Masculino , Pessoa de Meia-Idade , Traumatismo Múltiplo/cirurgia , Garantia da Qualidade dos Cuidados de Saúde , Estudos Retrospectivos , Centros de Traumatologia/estatística & dados numéricosRESUMO
A report is given on three women with ureterointestinal anastomosis after having a bladder exstrophy operation. Two of them carried out their pregnancies and had spontaneous deliveries. The problems of management during pregnancy are discussed and the literature is reviewed.
